SEC63 (NM_007214) Human Tagged ORF Clone

CAT#: RC208436

SEC63 (Myc-DDK-tagged)-Human SEC63 homolog (S. cerevisiae) (SEC63)

Product Images

Circular map for RC208436

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  Western blot validation of overexpression lysate (Cat# LY402111) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208436 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SEC63 protein (Cat# TP308436). The protein was produced from HEK293T cells transfected with SEC63 cDNA clone (Cat# RC208436) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC63
• Synonyms: DNAJC23; ERdj2; PCLD2; PRO2507; SEC63L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCAGCAGTTCCAGTACGATGACAGTGGGAACACCTTCTTCTACTTCCTCACCTCCTTCGTGG
  GGCTCATCGTGATCCCGGCGACATACTACCTCTGGCCCCGAGATCAGAATGCCGAGCAAATTCGATTAAA
  GAATATCAGAAAAGTATATGGAAGGTGTATGTGGTATCGTTTACGGTTATTAAAACCCCAGCCAAATATT
  ATTCCTACAGTAAAGAAAATAGTTCTGCTTGCAGGATGGGCATTGTTCTTATTCCTTGCATATAAAGTTT
  CCAAAACAGACCGAGAATACCAAGAATACAATCCTTATGAAGTATTAAATTTGGATCCTGGAGCCACAGT
  AGCAGAAATTAAAAAACAATATCGTTTGCTGTCACTTAAATATCATCCAGATAAAGGAGGTGATGAGGTT
  ATGTTCATGAGGATAGCAAAAGCTTATGCTGCTTTAACGGATGAAGAGTCCCGGAAAAATTGGGAAGAAT
  TTGGAAATCCAGATGGGCCTCAAGCCACAAGCTTTGGAATTGCCCTGCCAGCTTGGATAGTTGACCAGAA
  AAACTCAATTCTGGTTTTACTTGTATATGGATTGGCATTTATGGTTATCCTTCCAGTTGTTGTGGGCTCT
  TGGTGGTATCGCTCAATACGCTATAGTGGAGACCAGATTCTAATACGCACAACACAGATTTATACATACT
  TTGTTTATAAAACCCGAAATATGGATATGAAACGTCTTATCATGGTTTTGGCTGGAGCTTCTGAATTTGA
  TCCTCAGTATAATAAAGATGCCACAAGCAGACCAACGGATAATATTCTAATACCACAGCTAATCAGAGAA
  ATTGGCAGCATTAATTTAAAGAAGAATGAGCCTCCACTTACCTGCCCATATAGCCTGAAGGCCAGAGTTC
  TTTTACTGTCTCATCTTGCTAGAATGAAAATTCCTGAGACCCTTGAAGAAGATCAGCAATTCATGCTAAA
  AAAGTGTCCTGCCCTACTTCAAGAAATGGTTAATGTAATCTGCCAACTAATAGTAATGGCCCGGAACCGT
  GAAGAAAGGGAGTTTCGTGCTCCAACTTTGGCATCCCTAGAAAACTGCATGAAGCTTTCTCAGATGGCCG
  TTCAGGGACTTCAGCAATTTAAGTCTCCCCTTCTGCAGCTCCCTCATATTGAAGAGGACAATCTTAGACG
  GGTTTCTAATCATAAGAAGTATAAAATTAAAACTATCCAGGATTTGGTGAGTTTAAAAGAGTCAGATCGT
  CACACTCTACTGCACTTCCTTGAAGATGAAAAATATGAAGAGGTTATGGCTGTCCTTGGGAGTTTTCCAT
  ATGTGACCATGGATATAAAATCACAGGTGTTAGATGATGAAGATAGCAACAACATCACAGTAGGATCCTT
  AGTTACAGTGTTGGTTAAGTTGACAAGGCAAACAATGGCTGAAGTATTTGAAAAGGAGCAGTCCATCTGT
  GCTGCAGAGGAACAGCCAGCAGAAGATGGGCAGGGTGAAACTAACAAGAACAGGACAAAAGGAGGATGGC
  AACAGAAGAGTAAAGGACCCAAGAAAACTGCTAAATCAAAAAAAAAGAAACCTTTAAAAAAAAAACCTAC
  ACCTGTGCTATTACCACAGTCAAAGCAACAGAAACAAAAGCAGGCAAATGGAGTCGTTGGGAATGAAGCT
  GCAGTAAAGGAAGATGAAGAAGAAGTTTCAGATAAGGGCAGTGATTCTGAAGAAGAAGAAACCAATAGAG
  ATTCCCAAAGTGAGAAAGATGATGGTAGTGACAGAGACTCTGATAGAGAGCAAGATGAAAAACAAAACAA
  AGATGATGAAGCAGAGTGGCAAGAATTACAACAAAGCATACAGCGAAAAGAGAGAGCTCTATTGGAAACC
  AAATCAAAAATAACACATCCTGTGTATAGCCTTTACTTTCCTGAGGAAAAACAAGAATGGTGGTGGCTTT
  ACATTGCAGATAGGAAGGAGCAGACATTAATATCCATGCCATATCATGTGTGTACGCTGAAAGATACAGA
  GGAGGTAGAGCTGAAGTTTCCTGCACCAGGCAAGCCTGGAAATTATCAGTATACTGTGTTTCTGAGATCA
  GACTCCTATATGGGTTTGGATCAGATTAAACCATTGAAGTTGGAAGTTCATGAGGCTAAGCCTGTGCCAG
  AAAATCACCCACAGTGGGATACAGCAATAGAGGGGGATGAAGACCAGGAGGACAGTGAGGGCTTTGAAGA
  TAGCTTTGAGGAAGAAGAGGAGGAAGAAGAAGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGQQFQYDDSGNTFFYFLTSFVGLIVIPATYYLWPRDQNAEQIRLKNIRKVYGRCMWYRLRLLKPQPNI
  IPTVKKIVLLAGWALFLFLAYKVSKTDREYQEYNPYEVLNLDPGATVAEIKKQYRLLSLKYHPDKGGDEV
  MFMRIAKAYAALTDEESRKNWEEFGNPDGPQATSFGIALPAWIVDQKNSILVLLVYGLAFMVILPVVVGS
  WWYRSIRYSGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIRE
  IGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNR
  EEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDR
  HTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSIC
  AAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEA
  AVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLET
  KSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRS
  DSYMGLDQIKPLKLEVHEAKPVPENHPQWDTAIEGDEDQEDSEGFEDSFEEEEEEEEDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007214
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007214.5
• RefSeq Size: 6500 bp
• RefSeq ORF: 2283 bp
• Locus ID: 11231
• UniProt ID: Q9UGP8
• Cytogenetics: 6q21
• Domains: DnaJ, Sec63
• Protein Families: Transmembrane
• MW: 88 kDa
• Gene Summary: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]