Hemogen (HEMGN) (NM_018437) Human Tagged ORF Clone

CAT#: RC208367

HEMGN (Myc-DDK-tagged)-Human hemogen (HEMGN), transcript variant 1

Product Images

Circular map for RC208367

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HEMGN (Cat# RC208367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HEMGN(Cat# TA800885). Positive lysates LY402697 (100ug) and LC402697 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402697) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208367 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HEMGN protein (Cat# TP308367). The protein was produced from HEK293T cells transfected with HEMGN cDNA clone (Cat# RC208367) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hemogen
• Synonyms: CT155; EDAG; EDAG-1; NDR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208367 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGGGAAAGGACCAATCTCATTTGAAGCACCATCAGACACCTGACCCTCATCAAGAAGAGAACC
  ATTCTCCAGAAGTCATTGGAACCTGGAGTTTGAGAAACAGAGAACTACTTAGAAAAAGAAAAGCTGAAGT
  GCATGAAAAGGAAACATCACAATGGCTATTTGGAGAACAGAAAAAACGCAAGCAGCAGAGAACAGGAAAA
  GGAAATCGAAGAGGCAGAAAGAGACAACAAAACACAGAATTGAAGGTGGAGCCTCAGCCACAGATAGAAA
  AGGAAATGGAGAAAGCACTGGCACCTATAGAGAAAAAAACTGAGCCACCTGGGAGCATAACCAAAGTATT
  TCCTTCAGTAGCCTCCCCGCAAAAAGTTGTGCCTGAGGAACACTTTTCTGAAATATGTCAAGAAAGTAAC
  ATATATCAGGAGAATTTTTCTGAGTACCAAGAAATAGCAGTACAAAACCATTCTTCTGAAACATGCCAAC
  ATGTGTCTGAACCTGAAGACCTCTCTCCTAAAATGTACCAAGAAATATCTGTACTTCAAGACAATTCTTC
  CAAAATATGCCAAGACATGAAGGAACCTGAAGACAACTCTCCTAACACATGCCAAGTAATATCTGTAATT
  CAAGACCATCCTTTCAAAATGTACCAAGATATGGCTAAACGAGAAGATCTGGCTCCTAAAATGTGCCAAG
  AAGCTGCTGTACCCAAAATCCTTCCTTGTCCAACATCTGAAGACACAGCTGATCTGGCAGGATGCTCTCT
  TCAAGCATATCCAAAACCAGATGTGCCTAAAGGCTATATTCTTGACACAGACCAAAATCCAGCAGAACCA
  GAGGAATACAATGAAACAGATCAAGGAATAGCTGAGACAGAAGGCCTTTTTCCTAAAATACAAGAAATAG
  CTGAGCCTAAAGACCTTTCTACAAAAACACACCAAGAATCAGCTGAACCTAAATACCTTCCTCATAAAAC
  ATGTAACGAAATTATTGTGCCTAAAGCCCCCTCTCATAAAACAATCCAAGAAACACCTCATTCTGAAGAC
  TATTCAATTGAAATAAACCAAGAAACTCCTGGGTCTGAAAAATATTCACCTGAAACGTATCAAGAAATAC
  CTGGGCTTGAAGAATATTCACCTGAAATATACCAAGAAACATCCCAGCTTGAAGAATATTCACCTGAAAT
  ATACCAAGAAACACCGGGGCCTGAAGACCTCTCTACTGAGACATATAAAAATAAGGATGTGCCTAAAGAA
  TGCTTTCCAGAACCACACCAAGAAACAGGTGGGCCCCAAGGCCAGGATCCTAAAGCACACCAGGAAGATG
  CTAAAGATGCTTATACTTTTCCTCAAGAAATGAAAGAAAAACCCAAAGAAGAGCCAGGAATACCAGCAAT
  TCTGAATGAGAGTCATCCAGAAAATGATGTCTATAGTTATGTTTTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208367 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGK
  GNRRGRKRQQNTELKVEPQPQIEKEMEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESN
  IYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVI
  QDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEP
  EEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSED
  YSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKE
  CFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018437
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018437.5
• RefSeq Size: 2252 bp
• RefSeq ORF: 1455 bp
• Locus ID: 55363
• UniProt ID: Q9BXL5
• Cytogenetics: 9q22.33
• MW: 55.3 kDa
• Gene Summary: Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).[UniProtKB/Swiss-Prot Function]