CD3E (NM_000733) Human Tagged ORF Clone
CD3E (Myc-DDK-tagged)-Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E)
|Product Name||CD3E (NM_000733) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||IMD18; T3E; TCRE|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC208276 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208276 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||621 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000733.1, NM_000733.2, NM_000733.3, NP_000724.1|
|RefSeq Size||1534 bp|
|RefSeq ORF||624 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway|
|Gene Summary||The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
A novel method to generate T-cell receptor-deficient chimeric antigen receptor T cells
,Kamiya, T;Wong, D;Png, YT;Campana, D;,
Blood Adv 2018
,PubMed ID 29507075
|cDNA Clone Resources|
|SC128125||CD3E (untagged)-Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E)||
|RG208276||CD3E (GFP-tagged) - Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E)||
|RC208276L1||Lenti ORF clone of Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E), Myc-DDK-tagged||
|RC208276L2||Lenti ORF clone of Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E), mGFP tagged||
|RC208276L3||Lenti ORF clone of Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E), Myc-DDK-tagged||
|RC208276L4||Lenti ORF clone of Human CD3e molecule, epsilon (CD3-TCR complex) (CD3E), mGFP tagged||