DRAK1 (STK17A) (NM_004760) Human Tagged ORF Clone

CAT#: RC208265

STK17A (Myc-DDK-tagged)-Human serine/threonine kinase 17a (STK17A)

Product Images

Circular map for RC208265

• 

  Western blot validation of overexpression lysate (Cat# LY417773) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208265 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DRAK1
• Synonyms: DRAK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208265 representing NM_004760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCGGGCCGGGCAG
  GCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGCGGGCTGCTGACAGAGATACG
  CGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGCCTGTGCCCGGGCCGGGAGCTGGGCAGGGGG
  AAATTTGCAGTGGTGAGAAAATGTATAAAGAAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAA
  AAAGAAGAAAAGGCCAAGATTGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGA
  CAATCCTTGGGTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
  GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAAAAAGATGTTC
  AAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGTGATGTAGTTCATCTTGATTT
  GAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTGGGTGACATTAAGATTGTTGATTTTGGCCTT
  TCAAGAATATTGAAGAACAGTGAAGAGCTCCGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAA
  TTCTTAGTTATGATCCTATAAGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCT
  TACAGGAATATCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
  AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACACTTTTAGTTA
  AGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGGTTGACACAGAGCAGTATTCA
  AGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCAAATGCCCTCCAAGAAGGTCATTCTGTGCCT
  GAAATTAATTCGGATACCGACAAATCAGAAACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTA
  CTTCATATACTCTAGGACAATGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACG
  ATTTAAATTTGAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208265 representing NM_004760
  Red=Cloning site Green=Tags(s)
  
  MIPLEKPGSGGSSPGATSGSGRAGRGLSGPCRPPPPPQARGLLTEIRAVVRTEPFQDGYSLCPGRELGRG
  KFAVVRKCIKKDSGKEFAAKFMRKRRKGQDCRMEIIHEIAVLELAQDNPWVINLHEVYETASEMILVLEY
  AAGGEIFDQCVADREEAFKEKDVQRLMRQILEGVHFLHTRDVVHLDLKPQNILLTSESPLGDIKIVDFGL
  SRILKNSEELREIMGTPEYVAPEILSYDPISMATDMWSIGVLTYVMLTGISPFLGNDKQETFLNISQMNL
  SYSEEEFDVLSESAVDFIRTLLVKKPEDRATAEECLKHPWLTQSSIQEPSFRMEKALEEANALQEGHSVP
  EINSDTDKSETEESIVTEELIVVTSYTLGQCRQSEKEKMEQKAISKRFKFEEPLLQEIPGEFIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004760
• ORF Size: 1242 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004760.1, NP_004751.1
• RefSeq Size: 2641 bp
• RefSeq ORF: 1245 bp
• Locus ID: 9263
• UniProt ID: Q9UEE5
• Cytogenetics: 7p13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 46.4 kDa
• Gene Summary: This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq, Jul 2008]