DRAK1 (STK17A) (NM_004760) Human Untagged Clone

CAT#: SC323411

STK17A (untagged)-Kinase deficient mutant (K90M) of Human serine/threonine kinase 17a (STK17A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DRAK1
• Synonyms: DRAK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323411 sequence for NM_004760 edited (data generated by NextGen Sequencing)
  ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
  GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
  GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
  CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
  AAAGATTCTGGGAAAGAATTTGCTGCAATGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
  TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
  GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
  GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
  AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
  GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
  GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
  CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
  AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
  TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
  AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
  CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
  TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
  AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
  ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
  TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
  GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTGA
  
  Clone variation with respect to NM_004760.2
  269 a=>t;1084 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004760 unedited
  ACCGCCGTTGTAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCTGGGGAGAG
  CGGGTGTTTGAAGGCTCCGCGGACCGGCACTAGGAGCCGGGGGCGGGTCCGTGACCCTCCGGCTGCTCGG
  AGCGAACAGGCGGCCAGGAAAGAAGCGGGCCTGAACACCATGATCCCTTTGGAGAAGCCAGGCAGCGGCG
  GCTCCTCCCCAGGCGCCACCTCAGGCTCGGGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCC
  GCCGCCGCCCCAGGCCCGCGGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGAC
  GGCTACCAGCCTGGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGGTGGAGAAAAGGTTAA
  AGAAGGATCTGGGAAAGAATTTGCTGCAATGTTCATGAGAAAAGAAGAAAAGGCAAGATGGTCGGATGGA
  AATAATCATAAAATGCTTTACTTGAACTAGACAGGACATCCTTGGGTCCTTAATTTACCTGAAGTTTTTA
  GACTGACTACAGAATGTTCTTAGTCTGGATAGCTGTCTGGCGTGAATCTTGACCATGGTGTCAACCAGAG
  AAAGCCTTAGTAAGAGTTCAAGACTTTGCCAGATTGAAGGTCACTTACACCCGATGATTACTAGTGAGCC
  GGAATCCCGTGACTGATCCTTGGACTTAATGAATAGCTCGAGAATGACTTAAAAATCTAGGATGGAACTG
  GACTAGCTGAAACTCCGATGAGACCAACACTCGCCACCAG
  
  Kinase domain sequence

  >SC323411 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCTGGGGAGAGCGGGTG
  TTTGAAGGCTCCGCGGACCGGCACTAGGAGCCGGGGGCGGGTCCGTGACCCTCCGGCTGCTCGGAGCGAA
  CAGGCGGCCAGGAAAGAAGCGGGCCTGAACACCATGATCCCTTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_004760
• Insert Size: 3180 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004760.1, NP_004751.1
• RefSeq Size: 2641 bp
• RefSeq ORF: 1245 bp
• Locus ID: 9263
• UniProt ID: Q9UEE5
• Cytogenetics: 7p13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq, Jul 2008]