GFM1 (NM_024996) Human Tagged ORF Clone

CAT#: RC208239

GFM1 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC208239

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  Western blot validation of overexpression lysate (Cat# LY410910) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208239 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GFM1 protein (Cat# TP308239). The protein was produced from HEK293T cells transfected with GFM1 cDNA clone (Cat# RC208239) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GFM1
• Synonyms: COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1; mtEF-G1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208239 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTCCTGGGAGCTGCAGCCGTCGCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCCCTAGGCTGGC
  AGAGGAAGCAGGTTAATTGGAAGGCCTGCCGATGGTCTTCATCAGGGGTGATTCCTAATGAAAAAATACG
  AAATATTGGAATCTCAGCTCACATTGATTCTGGGAAAACTACATTAACAGAACGAGTCCTTTACTACACT
  GGCAGAATTGCAAAGATGCATGAGGTGAAAGGTAAAGATGGAGTTGGTGCTGTCATGGATTCCATGGAAC
  TAGAGAGACAAAGAGGAATCACTATTCAGTCAGCAGCCACTTACACCATGTGGAAAGATGTCAATATTAA
  CATTATAGATACTCCTGGGCATGTGGACTTCACAATAGAAGTGGAAAGGGCCCTGAGAGTGTTGGATGGT
  GCAGTCCTTGTTCTCTGTGCTGTTGGAGGGGTACAGTGCCAGACCATGACTGTCAATCGTCAGATGAAGC
  GCTACAACGTTCCGTTTCTAACTTTTATTAACAAATTGGACCGAATGGGCTCCAACCCAGCCAGGGCCCT
  GCAGCAAATGAGGTCTAAACTAAATCATAATGCAGCGTTTATGCAGATACCCATGGGTTTGGAGGGTAAT
  TTTAAAGGTATTGTAGATCTTATTGAGGAACGAGCCATCTATTTTGATGGAGACTTTGGTCAGATTGTTC
  GATATGGTGAGATTCCAGCTGAATTAAGGGCGGCGGCCACTGACCACCGGCAGGAGCTAATTGAATGTGT
  TGCCAATTCAGATGAACAGCTTGGTGAGATGTTTCTGGAAGAAAAAATCCCCTCGATTTCTGATTTAAAG
  CTAGCAATTCGAAGAGCTACTCTGAAAAGATCATTTACTCCTGTATTTTTGGGAAGCGCCTTGAAGAACA
  AAGGAGTTCAGCCTCTTTTAGATGCTGTTTTAGAATACCTCCCAAATCCATCTGAAGTCCAGAACTATGC
  TATTCTCAATAAAGAGGATGACTCAAAAGAGAAAACCAAAATCCTAATGAACTCCAGTAGAGACAATTCC
  CACCCATTTGTAGGCCTGGCTTTTAAACTGGAGGTAGGTCGATTTGGACAATTAACTTATGTTCGCAGTT
  ATCAGGGAGAGCTAAAGAAGGGTGACACCATCTATAACACAAGGACAAGAAAGAAAGTACGGTTGCAACG
  GCTGGCTCGCATGCATGCCGACATGATGGAGGATGTTGAGGAAGTATATGCCGGAGACATCTGTGCATTG
  TTTGGCATTGACTGTGCTAGTGGAGACACATTCACAGACAAAGCCAACAGCGGCCTTTCTATGGAGTCAA
  TTCATGTTCCTGATCCTGTCATTTCAATAGCAATGAAGCCTTCTAACAAGAACGATCTGGAAAAATTTTC
  AAAAGGTATTGGCAGGTTTACAAGAGAAGATCCCACATTTAAAGTATACTTTGACACTGAGAACAAAGAG
  ACAGTTATATCTGGAATGGGAGAATTACACCTGGAAATCTATGCTCAGAGGCTGGAAAGAGAGTATGGCT
  GTCCTTGTATCACAGGAAAGCCAAAAGTTGCCTTTCGAGAGACCATTACTGCCCCTGTCCCGTTTGACTT
  TACACATAAAAAACAATCAGGTGGTGCAGGCCAGTATGGAAAAGTAATAGGTGTCCTGGAGCCTCTGGAC
  CCAGAGGACTACACTAAATTGGAATTTTCAGATGAAACATTCGGATCAAATATTCCAAAGCAGTTTGTGC
  CTGCTGTAGAAAAGGGGTTTTTAGATGCCTGCGAGAAGGGCCCTCTTTCTGGTCACAAGCTCTCTGGGCT
  CCGGTTTGTCCTGCAAGATGGAGCACACCACATGGTTGATTCTAATGAAATCTCTTTCATCCGAGCAGGA
  GAAGGTGCTCTTAAACAAGCCTTGGCAAATGCAACATTATGTATTCTTGAACCTATTATGGCTGTGGAAG
  TTGTAGCTCCAAATGAATTTCAGGGACAAGTAATTGCAGGAATTAACCGACGCCATGGGGTAATCACTGG
  GCAAGATGGAGTTGAGGACTATTTTACACTGTATGCAGATGTCCCTCTAAATGATATGTTTGGTTATTCC
  ACTGAACTTAGGTCATGCACAGAGGGAAAGGGAGAATACACAATGGAGTATAGCAGGTATCAGCCATGTT
  TACCATCCACACAAGAAGACGTCATTAATAAGTATTTGGAAGCTACAGGTCAACTTCCTGTTAAAAAAGG
  AAAAGCCAAGAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208239 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLLGAAAVAALGRGRAPASLGWQRKQVNWKACRWSSSGVIPNEKIRNIGISAHIDSGKTTLTERVLYYT
  GRIAKMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTMWKDVNINIIDTPGHVDFTIEVERALRVLDG
  AVLVLCAVGGVQCQTMTVNRQMKRYNVPFLTFINKLDRMGSNPARALQQMRSKLNHNAAFMQIPMGLEGN
  FKGIVDLIEERAIYFDGDFGQIVRYGEIPAELRAAATDHRQELIECVANSDEQLGEMFLEEKIPSISDLK
  LAIRRATLKRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNKEDDSKEKTKILMNSSRDNS
  HPFVGLAFKLEVGRFGQLTYVRSYQGELKKGDTIYNTRTRKKVRLQRLARMHADMMEDVEEVYAGDICAL
  FGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKE
  TVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLD
  PEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAG
  EGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYS
  TELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024996
• ORF Size: 2253 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024996.7
• RefSeq Size: 3468 bp
• RefSeq ORF: 2256 bp
• Locus ID: 85476
• UniProt ID: Q96RP9
• Cytogenetics: 3q25.32
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
• MW: 83.5 kDa
• Gene Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008]