GALNT7 (NM_017423) Human Tagged ORF Clone

CAT#: RC208199

GALNT7 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) (GALNT7)

Product Images

Circular map for RC208199

• 

  Western blot validation of overexpression lysate (Cat# LY413781) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208199 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT7
• Synonyms: GALNAC-T7; GalNAcT7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208199 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGAAGATTGGATTCATCTTACGCAGTTTGCTGGTGGTGGGAAGCTTCCTGGGGCTAGTGGTCC
  TCTGGTCTTCCCTGACCCCGCGGCCGGACGACCCAAGCCCGCTGAGCAGGATGAGGGAAGACAGAGATGT
  CAATGACCCCATGCCCAACCGAGGCGGCAATGGACTAGCTCCTGGGGAGGACAGATTCAAACCTGTGGTA
  CCATGGCCTCATGTTGAAGGAGTAGAAGTGGACTTAGAGTCTATTAGAAGAATAAACAAGGCCAAAAATG
  AACAAGAGCACCATGCTGGAGGAGATTCCCAGAAAGATATCATGCAGAGGCAGTATCTCACATTTAAGCC
  TCAGACATTCACCTACCATGATCCTGTGCTTCGCCCAGGGATCCTCGGTAACTTTGAACCCAAAGAACCT
  GAGCCTCCTGGAGTGGTTGGTGGCCCTGGAGAGAAAGCCAAGCCATTGGTTTTGGGACCAGAATTCAAAC
  AAGCAATTCAAGCCAGCATTAAAGAGTTTGGATTTAACATGGTGGCAAGTGACATGATCTCACTGGACCG
  CAGCGTCAATGACTTACGCCAAGAAGAATGCAAGTATTGGCATTATGATGAAAACTTGCTCACTTCGAGC
  GTTGTCATTGTCTTCCATAATGAAGGATGGTCAACCCTCATGAGAACAGTCCACAGTGTAATTAAAAGGA
  CTCCAAGGAAATATTTAGCAGAAATTGTGTTAATTGACGATTTCAGTAATAAAGAACACTTAAAAGAAAA
  ACTGGATGAATATATTAAGCTGTGGAATGGCCTAGTGAAGGTATTTCGAAATGAAAGAAGGGAAGGTTTA
  ATTCAAGCACGAAGTATTGGTGCTCAGAAGGCTAAACTTGGACAGGTTTTGATATACCTTGATGCCCACT
  GTGAGGTGGCAGTTAACTGGTATGCACCACTTGTAGCTCCCATATCTAAGGACAGAACCATTTGCACTGT
  GCCGCTTATAGATGTCATAAATGGCAACACATATGAAATTATACCCCAAGGGGGTGGTGATGAAGATGGG
  TATGCCCGAGGAGCATGGGATTGGAGTATGCTCTGGAAACGGGTGCCTCTGACCCCTCAAGAGAAGAGAC
  TGAGAAAGACAAAAACTGAACCGTATCGGTCCCCAGCCATGGCTGGGGGATTATTTGCCATTGAACGAGA
  GTTCTTCTTTGAATTGGGTCTCTATGATCCAGGTCTCCAGATTTGGGGTGGTGAAAACTTTGAGATCTCA
  TACAAGATATGGCAGTGTGGTGGCAAATTATTATTTGTTCCTTGTTCTCGTGTTGGACATATCTACCGTC
  TTGAGGGCTGGCAAGGAAATCCTCCGCCCATTTATGTTGGGTCTTCTCCAACTCTGAAGAATTATGTTAG
  AGTTGTGGAGGTTTGGTGGGATGAATATAAAGACTACTTCTATGCTAGTCGTCCTGAATCGCAGGCATTA
  CCATATGGGGATATATCGGAGCTGAAAAAATTTCGAGAAGATCACAACTGCAAAAGTTTTAAGTGGTTCA
  TGGAAGAAATAGCTTATGATATCACCTCACACTACCCTTTGCCACCCAAAAATGTTGACTGGGGAGAAAT
  CAGAGGCTTCGAAACTGCTTACTGCATTGATAGCATGGGAAAAACAAATGGAGGCTTTGTTGAACTAGGA
  CCCTGCCACAGGATGGGAGGGAATCAGCTTTTCAGAATCAATGAAGCAAATCAACTCATGCAGTATGACC
  AGTGTTTGACAAAGGGAGCTGATGGATCAAAAGTTATGATTACACACTGTAATCTAAATGAATTTAAGGA
  ATGGCAGTACTTCAAGAACCTGCACAGATTTACTCATATTCCTTCAGGAAAGTGTTTAGATCGCTCAGAG
  GTCCTGCATCAAGTATTCATCTCCAATTGTGACTCCAGTAAAACGACTCAAAAATGGGAAATGAATAACA
  TCCATAGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208199 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLKIGFILRSLLVVGSFLGLVVLWSSLTPRPDDPSPLSRMREDRDVNDPMPNRGGNGLAPGEDRFKPVV
  PWPHVEGVEVDLESIRRINKAKNEQEHHAGGDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEP
  EPPGVVGGPGEKAKPLVLGPEFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSS
  VVIVFHNEGWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNERREGL
  IQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDG
  YARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEIS
  YKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQAL
  PYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELG
  PCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSE
  VLHQVFISNCDSSKTTQKWEMNNIHSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017423
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017423.3
• RefSeq Size: 4339 bp
• RefSeq ORF: 1974 bp
• Locus ID: 51809
• UniProt ID: Q86SF2
• Cytogenetics: 4q34.1
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 75.4 kDa
• Gene Summary: This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq, Jul 2008]