SYT9 (NM_175733) Human Tagged ORF Clone

CAT#: RC208183

SYT9 (Myc-DDK-tagged)-Human synaptotagmin IX (SYT9)

Product Images

Circular map for RC208183

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  Western blot validation of overexpression lysate (Cat# LY406245) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208183 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SYT9 protein (Cat# TP308183). The protein was produced from HEK293T cells transfected with SYT9 cDNA clone (Cat# RC208183) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYT9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208183 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGGGCCAGGGACGCGCTCTGTCACCAGGCGCTGCAGCTGCTGGCCGAGCTCTGTGCCCGTGGGG
  CCCTGGAGCACGACAGCTGCCAGGATTTCATTTACCACCTGCGGGACCGTGCCAGACCCCGGCTCCGCGA
  CCCAGATATCTCAGTGAGCCTGCTGACCCTTGTGGTCACTGCCTGTGGTCTCGCTCTCTTTGGCGTGTCT
  CTCTTCGTATCTTGGAAACTCTGCTGGGTTCCGTGGCGAGAACGAGGCCTGCCCTCTGGTAGCAAAGACA
  ACAACCAGGAGCCCCTTAACTACATGGACACAGAGACCAATGAGCAGGAGAACAGTGAGGACTTCCTAGA
  CCCTCCCACGCCCTGCCCTGACTCCTCCATGAAGATCAGCCACACCTCCCCTGACATTCCCCTCTCCACC
  CAGACGGGGATCCAGGAGAACTGTGCCCATGGCGTCCGCGTGCAGCGCCAAGTCACAGAGCCAACCTCGT
  CGGCCCGGCATAATTCAATCCGAAGACAACTCAACTTGTCAAACCCGGACTTCAATATCCAGCAGCTTCA
  AAAACAGGAACAGTTGACTGGAATTGGTAGAATTAAACCAGAGTTATATAAGCAGAGGTCATTGGATAAT
  GATGACGGGAGACGGAGTAACAGCAAGGCTTGTGGGAAACTGAACTTCATTTTAAAATATGACTGTGACT
  TAGAGCAGCTCATAGTGAAGATTCACAAAGCTGTCAATTTGCCCGCCAAGGACTTTTCTGGGACTTCAGA
  TCCTTATGTCAAGATCTATTTGCTTCCTGATCGGAAAACAAAACACCAGACTAAAGTTCACAGAAAGACC
  CTGAACCCTGTGTTTGATGAAGTGTTTTTATTTCCGGTTCCCTACAATGACCTTGAAGCACGGAAGCTTC
  ACTTCTCTGTGTACGACTTTGACAGGTTCTCTCGTCATGACTTAATCGGCCAAGTGGTGGTGGATCACTT
  CCTAGACTTGGCTGATTTCCCCAGGGAGTGCATCCTTTGGAAGGATATCGAATATGTCACCAATGACAAC
  GTGGATCTGGGAGAGCTGATGTTTTCCCTGTGCTATCTTCCAACGGCTGGCAGGCTGACCATTACCATTA
  TAAAAGCAAGGAATTTAAAGGCAATGGACATAACAGGAGCATCAGATCCCTATGTGAAAGTCTCGCTGAT
  GTGTGATGGCAGACGACTGAAGAAGAGGAAAACATCCACCAAGAGGAACACCTTGAATCCTGTTTACAAC
  GAAGCCATAGTCTTTGATGTCCCTCCCGAGAACATTGACCAAATCCACTTGTCCATAGCAGTCATGGACT
  ATGACCGTGTAGGTCACAATGAGATCATCGGCGTGTGTCAAGTAGGCAACGAGGCTGAGAGGCTGGGCAG
  AGACCACTGGAGTGAAATGTTGTCATATCCTCGGAAGCCCATTGCACACTGGCATTCCCTGGTGGAGAAA
  CGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208183 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGARDALCHQALQLLAELCARGALEHDSCQDFIYHLRDRARPRLRDPDISVSLLTLVVTACGLALFGVS
  LFVSWKLCWVPWRERGLPSGSKDNNQEPLNYMDTETNEQENSEDFLDPPTPCPDSSMKISHTSPDIPLST
  QTGIQENCAHGVRVQRQVTEPTSSARHNSIRRQLNLSNPDFNIQQLQKQEQLTGIGRIKPELYKQRSLDN
  DDGRRSNSKACGKLNFILKYDCDLEQLIVKIHKAVNLPAKDFSGTSDPYVKIYLLPDRKTKHQTKVHRKT
  LNPVFDEVFLFPVPYNDLEARKLHFSVYDFDRFSRHDLIGQVVVDHFLDLADFPRECILWKDIEYVTNDN
  VDLGELMFSLCYLPTAGRLTITIIKARNLKAMDITGASDPYVKVSLMCDGRRLKKRKTSTKRNTLNPVYN
  EAIVFDVPPENIDQIHLSIAVMDYDRVGHNEIIGVCQVGNEAERLGRDHWSEMLSYPRKPIAHWHSLVEK
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175733
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175733.3
• RefSeq Size: 3958 bp
• RefSeq ORF: 1476 bp
• Locus ID: 143425
• UniProt ID: Q86SS6
• Cytogenetics: 11p15.4
• Protein Families: Secreted Protein, Transmembrane
• MW: 56.2 kDa
• Gene Summary: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.[UniProtKB/Swiss-Prot Function]