GAS2L3 (NM_174942) Human Tagged ORF Clone

CAT#: RC208141

GAS2L3 (Myc-DDK-tagged)-Human growth arrest-specific 2 like 3 (GAS2L3)

Product Images

Circular map for RC208141

• 

  Western blot validation of overexpression lysate (Cat# LY406343) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208141 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS2L3
• Synonyms: G2L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208141 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCTGCAATTCAAGTATGGTTTGGAGAAGATCTGCCTCTAAGTCCTCGGAGTCCTCTGACTCCCA
  GACACGGACCAGGATTGGCTAATGTTTGTCAGTACGATGAGTGGATAGCTGTGAGGCATGAAGCCACTTT
  GTTGCCCATGCAAGAAGATCTGTCAATCTGGTTATCTGGTTTATTAGGTATTAAAGTTAAGGCAGAAAAA
  TTATTGGAAGAACTTGATAATGGAGTACTATTATGTCAACTGATTGATGTTCTTCAAAACATGGTGAAAA
  CATGCAACTCTGAAGAATCAGGGAATTTTCCAATGAGAAAAGTGCCCTGTAAGAAAGATGCTGCATCAGG
  TTCATTCTTTGCTCGGGACAATACCGCAAACTTCCTTCACTGGTGTAGGGACATTGGGGTTGATGAAACT
  TACCTCTTTGAATCTGAAGGTTTAGTTTTGCACAAAGATCCAAGACAGGTGTATCTTTGTCTTCTTGAAA
  TTGGTCGAATTGTGTCAAGATACGGGGTTGAGCCACCAGTGTTAGTAAAACTTGAGAAAGAAATTGAGTT
  AGAAGAGACTTTGCTTAATACTTCTGGGCCTGAAGATTCCATCAGCATTCCAAAATCATGCTGTCGGCAT
  GAAGAGCTACATGAAGCTGTTAAACATATTGCTGAGGACCCTCCTTGTAGTTGTTCTCATCGATTTTCTA
  TTGAGTATTTATCTGAAGGACGGTACCGACTAGGGGATAAAATACTCTTTATAAGAATGCTTCATGGAAA
  ACATGTCATGGTTCGCGTTGGTGGAGGCTGGGATACTCTTCAAGGATTTTTGCTTAAATATGACCCCTGT
  CGAATATTACAGTTTGCCACACTAGAACAAAAAATTTTAGCATTTCAAAAAGGAGTTTCTAATGAAAGTG
  TACCTGATTCGCCTGCCAGAACACCTCAGCCTCCTGAAATGAATCCTTTGTCAGCAGTTAACATGTTTCA
  GAAACAAAATTCAAAACCCAGCGTGCCAGTTAGTATTCCAAAAAGCAAAGAAAAACAGGGACGTCCACCA
  GGTGCATTGGTGCCAGCATCTTCACTGAAAGGAGGTAATCTGGGCTCTATGTCAGTCCGTTCTAAATTGC
  CAAATTCTCCAGCAGCATCTTCTCATCCCAAGCTCAAGTCTTCAAAAGGCATAACGAAGAAACCGCAGGC
  TCCTTCAAACAATGCATCATCTTCACTTGCTTCATTAAATCCAGTAGGTAAAAACACTTCTTCACCAGCT
  TTACCAAGAACTGCACCTTGTATATCTGAGTCACCGAGAAAATGTATTTCATCCCCCAATACCCCCAAGG
  CCAAGGTTATTCCAGCCCAGAATTCAGCAGATCTGCCCGAGTCCACACTTTTGCCAAATAAGTGTTCAGG
  AAAAACTCAACCTAAGTATTTGAAACATAATCATATTTCTTCCAGAGATAATGCAGTATCTCACTTAGCT
  GCACATTCAAATTCATCCTCAAAATGTCCCAAGCTGCCTAAAGCAAATATACCTGTAAGACCTAAACCTT
  CTTTCCAGTCCTCTGCAAAAATGACAAAAACCAGTTCCAAAACCATAGCCACGGGTCTAGGAACACAGTC
  TCAACCATCCGATGGAGCCCCACAAGCAAAGCCAGTCCCAGCACAGAAACTTAAATCGGCCTTGAATTTA
  AATCAGCCAGTTTCTGTGTCCTCAGTTTCTCCTGTAAAAGCCACACAGAAATCAAAAGATAAGAATATAG
  TTTCAGCTACCAAAAAGCAGCCTCAGAATAAAAGTGCATTTCAGAAGACAGGACCCAGCTCCTTGAAGTC
  TCCTGGCCGTACCCCACTGTCCATCGTGAGCCTACCCCAGTCTTCTACCAAAACACAAACTGCACCGAAG
  TCAGCACAGACTGTCGCTAAGAGCCAGCATTCAACTAAAGGGCCTCCCAGAAGTGGCAAAACCCCAGCTT
  CAATCAGGAAACCACCCTCATCTGTTAAGGATGCAGATAGTGGAGATAAAAAACCTACTGCAAAGAAAAA
  GGAAGATGATGACCATTATTTTGTCATGACTGGAAGTAAGAAACCTAGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208141 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQPAIQVWFGEDLPLSPRSPLTPRHGPGLANVCQYDEWIAVRHEATLLPMQEDLSIWLSGLLGIKVKAEK
  LLEELDNGVLLCQLIDVLQNMVKTCNSEESGNFPMRKVPCKKDAASGSFFARDNTANFLHWCRDIGVDET
  YLFESEGLVLHKDPRQVYLCLLEIGRIVSRYGVEPPVLVKLEKEIELEETLLNTSGPEDSISIPKSCCRH
  EELHEAVKHIAEDPPCSCSHRFSIEYLSEGRYRLGDKILFIRMLHGKHVMVRVGGGWDTLQGFLLKYDPC
  RILQFATLEQKILAFQKGVSNESVPDSPARTPQPPEMNPLSAVNMFQKQNSKPSVPVSIPKSKEKQGRPP
  GALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPA
  LPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLA
  AHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNL
  NQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPK
  SAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174942
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174942.1, NP_777602.1
• RefSeq Size: 2341 bp
• RefSeq ORF: 2085 bp
• Locus ID: 283431
• UniProt ID: Q86XJ1
• Cytogenetics: 12q23.1
• MW: 75.2 kDa
• Gene Summary: Cytoskeletal linker protein. May promote and stabilize the formation of the actin and microtubule network.[UniProtKB/Swiss-Prot Function]