DNAJC3 (NM_006260) Human Tagged ORF Clone

CAT#: RC208126

DNAJC3 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 3 (DNAJC3)

Product Images

Circular map for RC208126

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  Western blot validation of overexpression lysate (Cat# LY416762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208126 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAJC3 protein (Cat# TP308126). The protein was produced from HEK293T cells transfected with DNAJC3 cDNA clone (Cat# RC208126) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJC3
• Synonyms: ACPHD; ERdj6; HP58; P58; p58(IPK); P58IPK; PRKRI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208126 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCCCCGGCTCCGTGACCAGCCGGCTGGGCTCGGTATTCCCCTTCCTGCTAGTCCTGGTGGATC
  TGCAGTACGAAGGTGCTGAATGTGGAGTAAATGCAGATGTTGAGAAACATCTTGAATTGGGCAAGAAATT
  ACTTGCAGCTGGACAGCTAGCTGATGCTTTATCTCAGTTTCATGCTGCCGTAGATGGTGACCCTGATAAC
  TATATTGCTTATTATCGGAGGGCTACTGTCTTTTTAGCTATGGGCAAATCAAAAGCTGCACTTCCTGATT
  TAACTAAAGTGATTCAATTGAAGATGGACTTCACTGCAGCAAGATTACAGAGAGGTCACTTATTACTCAA
  ACAAGGAAAACTTGATGAAGCAGAAGATGATTTTAAAAAAGTGCTCAAATCTAATCCAAGTGAAAATGAA
  GAAAAGGAAGCACAGTCTCAACTTATAAAATCTGATGAAATGCAGCGTTTGCGTTCACAAGCACTTAACG
  CTTTTGGAAGTGGAGATTATACTGCTGCTATAGCCTTCCTTGATAAGATTTTAGAGGTTTGTGTTTGGGA
  TGCAGAACTACGGGAACTTCGAGCTGAATGTTTTATAAAAGAAGGAGAACCTAGGAAAGCTATAAGTGAC
  TTAAAAGCTGCGTCAAAGTTGAAGAATGATAATACTGAAGCGTTTTATAAAATAAGCACACTGTACTACC
  AACTAGGAGACCACGAACTGTCCCTCAGTGAAGTTCGGGAATGTCTTAAACTTGACCAGGATCATAAAAG
  GTGTTTTGCACACTATAAACAAGTAAAGAAACTTAATAAGCTGATTGAGTCAGCTGAAGAGCTCATCAGA
  GATGGCAGATACACAGATGCTACCAGCAAATATGAATCTGTCATGAAAACAGAGCCAAGCATTGCTGAAT
  ATACAGTTCGTTCAAAGGAGAGGATTTGCCACTGCTTTTCTAAGGACGAGAAGCCTGTTGAAGCTATTAG
  GGTTTGTTCTGAAGTTTTACAGATGGAACCTGACAATGTGAATGCCCTGAAAGATCGAGCAGAGGCCTAT
  TTGATAGAGGAAATGTATGATGAAGCTATTCAGGATTATGAAACTGCTCAGGAACACAATGAAAATGATC
  AGCAGATTCGAGAAGGTCTAGAGAAAGCACAAAGATTATTGAAACAGTCGCAGAAACGAGATTATTATAA
  AATCTTGGGAGTAAAAAGAAATGCCAAAAAGCAAGAAATTATTAAAGCATACCGAAAATTAGCACTGCAG
  TGGCACCCAGATAACTTCCAGAATGAAGAAGAAAAGAAAAAAGCTGAGAAAAAGTTCATTGATATAGCAG
  CTGCTAAAGAAGTCCTCTCTGATCCAGAAATGAGAAAGAAGTTTGACGACGGAGAAGATCCTTTGGATGC
  AGAGAGCCAGCAAGGAGGCGGCGGCAACCCTTTCCACAGAAGCTGGAACTCATGGCAAGGGTTCAATCCC
  TTCAGCTCAGGCGGACCATTTAGATTTAAATTCCACTTCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208126 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVAPGSVTSRLGSVFPFLLVLVDLQYEGAECGVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDN
  YIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENE
  EKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISD
  LKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIR
  DGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAY
  LIEEMYDEAIQDYETAQEHNENDQQIREGLEKAQRLLKQSQKRDYYKILGVKRNAKKQEIIKAYRKLALQ
  WHPDNFQNEEEKKKAEKKFIDIAAAKEVLSDPEMRKKFDDGEDPLDAESQQGGGGNPFHRSWNSWQGFNP
  FSSGGPFRFKFHFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006260
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006260.5
• RefSeq Size: 5598 bp
• RefSeq ORF: 1515 bp
• Locus ID: 5611
• UniProt ID: Q13217
• Cytogenetics: 13q32.1
• Domains: TPR, DnaJ
• MW: 57.6 kDa
• Gene Summary: This gene encodes a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR). [provided by RefSeq, Jul 2010]