TCP11L1 (NM_018393) Human Tagged ORF Clone

CAT#: RC208041

TCP11L1 (Myc-DDK-tagged)-Human t-complex 11 (mouse)-like 1 (TCP11L1), transcript variant 1

Product Images

Circular map for RC208041

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  Western blot validation of overexpression lysate (Cat# LY428932) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228003 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TCP11L1 protein (Cat# TP308041). The protein was produced from HEK293T cells transfected with TCP11L1 cDNA clone (Cat# RC208041) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCP11L1
• Synonyms: dJ85M6.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208041 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAAAACCTTGACAAGTCCAATGTAAATGAAGCAGGAAAATCAAAATCCAATGATTCTGAGGAAG
  GCCTCGAAGATGCTGTGGAAGGTGCTGATGAAGCCTTACAAAAAGCAATAAAGTCAGACTCCTCCAGCCC
  CCAAAGAGTGCAGAGACCTCACTCTAGTCCTCCTCGCTTTGTGACAGTAGAAGAACTTCTAGAGACAGCG
  AGAGGCGTCACCAACATGGCTCTAGCCCATGAAATTGTAGTAAATGGAGACTTTCAGATTAAACCAGTTG
  AATTACCAGAAAACAGCTTGAAGAAGAGAGTAAAGGAGATTGTACATAAAGCGTTTTGGGATTGCTTGAG
  TGTGCAGCTAAGTGAAGATCCCCCAGCATATGACCATGCTATCAAACTTGTAGGAGAAATCAAAGAGACT
  CTCTTATCTTTCTTGCTGCCTGGTCATACTAGACTGAGAAACCAGATAACAGAAGTCTTGGATCTGGATC
  TGATAAAGCAGGAAGCAGAGAATGGGGCGCTAGACATTTCCAGGCTGGCAGAATTCATTATTGGCATGAT
  GGGGACACTGTGTGCACCTGCTCGAGATGAGGAAGTTAAGAAACTAAAGGACATTAAGGAAATAGTGCCC
  CTTTTCAGAGAAATTTTTTCTGTGTTGGACCTAATGAAAGTGGACATGGCCAACTTTGCTATCAGTAGCA
  TCAGGCCTCATCTCATGCAGCAGTCAGTTGAATACGAAAGGAAGAAGTTTCAAGAGATTTTGGAGAGGCA
  ACCAAATTCCCTGGACTTTGTCACCCAGTGGCTGGAAGAAGCCTCAGAGGACCTTATGACTCAGAAGTAT
  AAACACGCCCTGCCAGTGGGGGGAATGGCTGCTGGCTCTGGGGACATGCCCAGGCTGAGCCCTGTTGCTG
  TCCAGAATTACGCTTACCTGAAGCTTCTGAAGTGGGACCACCTCCAGAGGCCGTTCCCCGAAACAGTTTT
  AATGGACCAGTCTCGCTTCCACGAGCTCCAGTTGCAGCTGGAACAACTGACCATCCTGGGGGCTGTGTTG
  CTGGTCACCTTCAGCATGGCAGCGCCAGGAATTTCCAGCCAGGCCGACTTTGCTGAGAAACTCAAGATGA
  TTGTGAAGATTTTGCTAACAGATATGCACCTGCCCTCCTTCCATCTGAAGGACGTCCTCACTACCATCGG
  GGAGAAGGTGTGCCTGGAGGTGAGCAGCTGCCTCTCCCTGTGTGGGTCCTCTCCCTTCACCACGGACAAG
  GAGACCGTGCTCAAGGGCCAGATCCAGGCCGTGGCCAGTCCCGATGACCCCATTCGCAGGATCATGGAAT
  CTCGAATCCTGACCTTCTTAGAAACCTACCTTGCCTCGGGTCATCAGAAGCCATTGCCCACAGTCCCTGG
  GGGACTCAGTCCAGTTCAGAGAGAGCTGGAGGAAGTTGCTATTAAATTTGCTCGCCTGGTCAACTATAAC
  AAGATGGTCTTCTGTCCCTACTACGATGCAATCCTGAGTAAGATCCTCGTCCGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208041 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSENLDKSNVNEAGKSKSNDSEEGLEDAVEGADEALQKAIKSDSSSPQRVQRPHSSPPRFVTVEELLETA
  RGVTNMALAHEIVVNGDFQIKPVELPENSLKKRVKEIVHKAFWDCLSVQLSEDPPAYDHAIKLVGEIKET
  LLSFLLPGHTRLRNQITEVLDLDLIKQEAENGALDISRLAEFIIGMMGTLCAPARDEEVKKLKDIKEIVP
  LFREIFSVLDLMKVDMANFAISSIRPHLMQQSVEYERKKFQEILERQPNSLDFVTQWLEEASEDLMTQKY
  KHALPVGGMAAGSGDMPRLSPVAVQNYAYLKLLKWDHLQRPFPETVLMDQSRFHELQLQLEQLTILGAVL
  LVTFSMAAPGISSQADFAEKLKMIVKILLTDMHLPSFHLKDVLTTIGEKVCLEVSSCLSLCGSSPFTTDK
  ETVLKGQIQAVASPDDPIRRIMESRILTFLETYLASGHQKPLPTVPGGLSPVQRELEEVAIKFARLVNYN
  KMVFCPYYDAILSKILVRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018393
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018393.2, NP_060863.2
• RefSeq Size: 2817 bp
• RefSeq ORF: 1530 bp
• Locus ID: 55346
• UniProt ID: Q9NUJ3
• Cytogenetics: 11p13
• MW: 57.1 kDa