ZDHHC17 (NM_015336) Human Tagged ORF Clone

CAT#: RC207982

ZDHHC17 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 17 (ZDHHC17)

Product Images

Circular map for RC207982

• 

  Western blot validation of overexpression lysate (Cat# LY402425) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207982 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZDHHC17
• Synonyms: DHHC-17; HIP3; HIP14; HSPC294; HYPH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207982 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGAGGAGGGATTTAACACCAAGATGGCGGACGGCCCGGATGAGTACGATACCGAAGCGGGCT
  GTGTGCCCCTTCTCCACCCAGAGGAAATCAAACCCCAAAGCCATTATAACCATGGATATGGTGAACCTCT
  TGGACGGAAAACTCATATTGATGATTACAGCACATGGGACATAGTCAAGGCTACACAATATGGAATATAT
  GAACGCTGTCGAGAATTGGTGGAAGCAGGTTATGATGTACGGCAACCGGACAAAGAAAATGTTACCCTCC
  TCCATTGGGCTGCCATCAATAACAGAATAGATTTAGTCAAATACTATATTTCGAAAGGTGCTATTGTGGA
  TCAACTTGGAGGGGACCTGAATTCAACTCCATTGCACTGGGCCACAAGACAAGGCCATCTATCCATGGTT
  GTGCAACTAATGAAATATGGTGCAGATCCTTCATTAATTGATGGAGAAGGATGTAGCTGTATTCATCTGG
  CTGCTCAGTTCGGACATACCTCAATTGTTGCTTATCTCATAGCAAAAGGACAGGATGTAGATATGATGGA
  TCAGAATGGAATGACGCCTTTAATGTGGGCAGCATATAGAACACATAGTGTGGATCCAACTAGATTGCTT
  TTAACATTCAATGTTTCAGTTAACCTTGGTGACAAGTATCACAAAAACACTGCTCTGCATTGGGCAGTGC
  TAGCAGGGAATACCACAGTCATTAGCCTTCTTCTGGAAGCTGGAGCTAATGTTGATGCCCAGAATATCAA
  GGGCGAATCAGCGCTTGATTTGGCAAAACAGAGAAAAAATGTGTGGATGATCAACCACTTACAAGAGGCA
  AGGCAAGCAAAAGGATATGACAATCCGTCCTTCCTTAGAAAGCTGAAAGCTGATAAGGAATTTCGGCAGA
  AAGTAATGTTAGGAACTCCTTTCCTAGTTATTTGGCTGGTTGGGTTTATAGCAGACCTAAATATTGATTC
  TTGGCTCATTAAAGGGCTAATGTATGGTGGTGTTTGGGCTACAGTACAGTTTCTTTCAAAATCCTTTTTC
  GATCATTCAATGCATAGTGCATTGCCCCTTGGGATATATTTGGCAACCAAATTCTGGATGTATGTGACGT
  GGTTCTTCTGGTTTTGGAATGATCTCAACTTTTTATTTATCCATCTTCCATTCCTTGCCAATAGTGTTGC
  ACTTTTCTACAATTTTGGAAAATCTTGGAAATCAGATCCAGGGATTATTAAAGCAACAGAAGAGCAAAAG
  AAAAAGACAATAGTTGAACTTGCAGAGACAGGAAGTCTGGACCTCAGTATATTCTGCAGTACCTGTTTGA
  TACGAAAACCGGTGAGGTCCAAACATTGTGGTGTGTGCAACCGCTGTATAGCAAAATTTGATCATCATTG
  CCCATGGGTGGGTAACTGTGTAGGTGCAGGCAACCATAGATATTTTATGGGCTACCTATTCTTCTTGCTT
  TTTATGATCTGCTGGATGATTTATGGTTGTATATCTTACTGGGGACTCCACTGTGAGACCACTTACACCA
  AGGATGGATTTTGGACATACATTACTCAGATTGCCACGTGTTCACCTTGGATGTTTTGGATGTTCCTGAA
  CAGTGTTTTCCACTTCATGTGGGTGGCTGTATTACTCATGTGTCAGATGTACCAGATATCATGTTTAGGT
  ATTACTACAAATGAAAGAATGAATGCCAGGAGATACAAGCACTTTAAAGTCACAACAACGTCTATTGAAA
  GCCCATTCAACCATGGATGTGTAAGAAATATTATAGACTTCTTTGAATTTCGATGCTGTGGCCTCTTTCG
  TCCTGTTATCGTGGACTGGACCAGGCAGTATACAATAGAATATGACCAAATATCAGGATCTGGGTACCAG
  CTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207982 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQREEGFNTKMADGPDEYDTEAGCVPLLHPEEIKPQSHYNHGYGEPLGRKTHIDDYSTWDIVKATQYGIY
  ERCRELVEAGYDVRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMV
  VQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHSVDPTRLL
  LTFNVSVNLGDKYHKNTALHWAVLAGNTTVISLLLEAGANVDAQNIKGESALDLAKQRKNVWMINHLQEA
  RQAKGYDNPSFLRKLKADKEFRQKVMLGTPFLVIWLVGFIADLNIDSWLIKGLMYGGVWATVQFLSKSFF
  DHSMHSALPLGIYLATKFWMYVTWFFWFWNDLNFLFIHLPFLANSVALFYNFGKSWKSDPGIIKATEEQK
  KKTIVELAETGSLDLSIFCSTCLIRKPVRSKHCGVCNRCIAKFDHHCPWVGNCVGAGNHRYFMGYLFFLL
  FMICWMIYGCISYWGLHCETTYTKDGFWTYITQIATCSPWMFWMFLNSVFHFMWVAVLLMCQMYQISCLG
  ITTNERMNARRYKHFKVTTTSIESPFNHGCVRNIIDFFEFRCCGLFRPVIVDWTRQYTIEYDQISGSGYQ
  LV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015336
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015336.3
• RefSeq Size: 4771 bp
• RefSeq ORF: 1899 bp
• Locus ID: 23390
• UniProt ID: Q8IUH5
• Cytogenetics: 12q21.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 72.6 kDa
• Gene Summary: Palmitoyltransferase specific for a subset of neuronal proteins, including SNAP25, DLG4/PSD95, GAD2, SYT1 and HTT (PubMed:15603740, PubMed:15489887, PubMed:19139280, PubMed:28757145). May be involved in the sorting or targeting of critical proteins involved in the initiating events of endocytosis at the plasma membrane (PubMed:12393793). May play a role in Mg(2+) transport (PubMed:18794299).[UniProtKB/Swiss-Prot Function]