KIAA1586 (NM_020931) Human Tagged ORF Clone

CAT#: RC207935

KIAA1586 (Myc-DDK-tagged)-Human KIAA1586 (KIAA1586)

Product Images

Circular map for RC207935

• 

  Western blot validation of overexpression lysate (Cat# LY412227) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207935 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIAA1586
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207935 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACCCGGGGTCGGAAATAATAGAATCTGTCCCTCCAGCTGGCCCTGAGGCATCTGAGTCAACAA
  CGGATGAAAATGAAGACGACATTCAGTTTGTCAGTGAAGGACCATCGAGACCTGTTCTTGAATACATCGA
  TCTGGTCTGTGGTGATGATGAAAACCCTAGCGCCTATTATAGTGATATTCTGTTTCCTAAAATGCCAAAA
  CGACAGGGTGATTTTTTGCATTTTTTAAATGTGAAGAAGGTGAAAACAGACACAGAAAATAATGAAGTGA
  GCAAAAATCACTGCAGATTGTCTAAGGCAAAGGAACCACATTTCGAGTATATTGAACAACCAATCATTGA
  AGAAAAGCCATCACTTTCATCAAAGAAAGAAATAGATAATCTTGTGCTTCCAGATTGTTGGAATGAAAAA
  CAAGCATTTATGTTTACAGAACAATACAAATGGCTTGAAATAAAAGAAGGTAAATTAGGATGTAAGGATT
  GTTCAGCAGTTCGGCATTTGGGATCGAAAGCAGAAAAGCATGTCCATGTGTCCAAGGAATGGATTGCATA
  TTTAGTAACCCCTAATGGCAGTAATAAAACTACTAGGCAAGCTTCTCTACGAAAAAAAATTAGGGAACAT
  GATGTTTCTAAAGCCCATGGTAAAATTCAGGATTTGTTAAAGGAATCAACTAATGATTCAATTTGTAATT
  TAGTGCATAAACAAAATAATAAAAATATTGATGCTACTGTAAAAGTTTTCAATACTGTTTACAGTTTAGT
  AAAACATAACAGACCTTTATCTGATATTGAGGGGGCAAGAGAATTACAGGAAAAAAATGGAGAGGTAAAT
  TGTTTAAATACACGTTACAGTGCAACAAGAATAGCAGAACATATTGCAAAAGAAATGAAGATGAAGATAT
  TTAAGAATATTATAGAAGAGAATGCCAAAATCTGTATCATAATTGATGAGGCATCTACAGTTTCAAAGAA
  AACCACCCTAGTGATTTATCTCCAGTGCACAATTCAGTCAGCTCCTGCACCTGTTATGTTATTTGTGGCT
  TTAAAAGAATTGGTGTCAACTATAGCAGAGTGTATTGTCAATACATTATTGACTACTTTAAATGATTGTG
  GTTTTACAAATGAATATTTGAAAGCAAATTTAATTGCATTTTGTTCTGATGGTGCTAATACAATCCTGGG
  AAGAAAGTCTGGAGTAGCTACAAAATTGTTAGAAAATTTTCCTGAAATCATCATTTGGAACTGTTTAAAT
  CATCGATTACAATTGTCACTTGATGATTCTATATCCGAAATAAAACAAATTAATCATTTAAAAATATTTA
  TTGATAAAATTTATTCTATTTATCATCAACCTAATAAAAATCAAACCAAGCTTCTAGGAACTGTAGCTAA
  AGAACTTGAAACTGAAATTATTAAAATTGGTCGAGTAATGGGACCAAGATGGGCGGCATGTAGTTTACAA
  GCTGCTACTGCTGTATGGCATGCATATCCTATATTATATATGCATTTTTCTCATTCTTACTCTGGTTTGG
  CGAAGAGATTAGCTAACATTAATTTCTTACAAGACCTTGCTTTAATGATTGACATTCTTGAAGAATTTTC
  AGTACTTTCAACTGCATTACAGTCAAGATCAACTAATATTAAGAAAGCACAAAAATTGATCAAACGTACC
  ATAAGAGCTTTGGAAAATTTAAAAATTGGTACTGGAAAGTATGAATCTCAAATTGAAGATTTGATCAAGT
  CAGATAAGTTTAAAGATATTCCATTTAATAAAAACAATAAATTTAATGCTCTTCCTAGGAGTATATTACT
  AGACAATATAATTCAGCACATGAACCTACGCCTTTTATCTGACAGAAACCATGAAGATATTTTTAATTAC
  TTTGATTTGCTGGAACCTTCCACATGGCCTTATGAAGAAATAACTTCACCATGGATAGCTGGTGAAAAAA
  CATTATTTCATTTGTGTAAAATTTTAAAATATGAAGTTGATTTGAATGATTTTCGGGAATTTGTAAATAA
  TAATATAAAATCAAACAATGTTTCAATTCCTACAACTATATACAAAGCTAAAAAGATAGTTAGCACCATT
  GCAATCAATAGTGCTGAAGCTGAAAGGGGTTTCAATTTAATGAACATAATTTGTACAAGGGTGAGAAATA
  GTTTAACAATAGATCATGTATCAGATTTAATGACAATAAATTTACTGGGGAAAGAATTAGCAGATTGGGA
  TGCAACACCGTTTGTAAAATCTTGGTCAAATTGCAACCACAGGTTGGCTACAGATACAAGAGTTCGGCAA
  AAGTCAACAAAAGTCTTCCATGAGAATCAATTGGCTATATGGAACTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207935 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDPGSEIIESVPPAGPEASESTTDENEDDIQFVSEGPSRPVLEYIDLVCGDDENPSAYYSDILFPKMPK
  RQGDFLHFLNVKKVKTDTENNEVSKNHCRLSKAKEPHFEYIEQPIIEEKPSLSSKKEIDNLVLPDCWNEK
  QAFMFTEQYKWLEIKEGKLGCKDCSAVRHLGSKAEKHVHVSKEWIAYLVTPNGSNKTTRQASLRKKIREH
  DVSKAHGKIQDLLKESTNDSICNLVHKQNNKNIDATVKVFNTVYSLVKHNRPLSDIEGARELQEKNGEVN
  CLNTRYSATRIAEHIAKEMKMKIFKNIIEENAKICIIIDEASTVSKKTTLVIYLQCTIQSAPAPVMLFVA
  LKELVSTIAECIVNTLLTTLNDCGFTNEYLKANLIAFCSDGANTILGRKSGVATKLLENFPEIIIWNCLN
  HRLQLSLDDSISEIKQINHLKIFIDKIYSIYHQPNKNQTKLLGTVAKELETEIIKIGRVMGPRWAACSLQ
  AATAVWHAYPILYMHFSHSYSGLAKRLANINFLQDLALMIDILEEFSVLSTALQSRSTNIKKAQKLIKRT
  IRALENLKIGTGKYESQIEDLIKSDKFKDIPFNKNNKFNALPRSILLDNIIQHMNLRLLSDRNHEDIFNY
  FDLLEPSTWPYEEITSPWIAGEKTLFHLCKILKYEVDLNDFREFVNNNIKSNNVSIPTTIYKAKKIVSTI
  AINSAEAERGFNLMNIICTRVRNSLTIDHVSDLMTINLLGKELADWDATPFVKSWSNCNHRLATDTRVRQ
  KSTKVFHENQLAIWNLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020931
• ORF Size: 2361 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020931.4
• RefSeq Size: 2964 bp
• RefSeq ORF: 2364 bp
• Locus ID: 57691
• UniProt ID: Q9HCI6
• Cytogenetics: 6p12.1
• MW: 89.7 kDa
• Gene Summary: E3 SUMO-protein ligase; facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PubMed:26524493).[UniProtKB/Swiss-Prot Function]