GTPBP4 (NM_012341) Human Tagged ORF Clone

CAT#: RC207903

GTPBP4 (Myc-DDK-tagged)-Human GTP binding protein 4 (GTPBP4)

Product Images

Circular map for RC207903

• 

  Western blot validation of overexpression lysate (Cat# LY415798) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207903 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTPBP4
• Synonyms: CRFG; NGB; NOG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207903 representing NM_012341
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACATTACAACTTCAAGAAAATTACGGTGGTGCCGTCCGCCAAGGACTTCATAGACCTCACGTTGT
  CGAAGACTCAACGAAAGACTCCAACCGTTATTCATAAACATTACCAAATACATCGCATTAGACATTTTTA
  CATGAGAAAAGTCAAATTTACTCAACAGAATTACCATGATAGACTTTCACAAATTCTAACAGATTTCCCC
  AAATTGGATGATATTCATCCGTTCTATGCTGATTTGATGAATATTCTCTACGACAAGGATCATTACAAGT
  TGGCTCTGGGGCAAATAAATATTGCCAAAAATTTAGTGGACAATGTTGCTAAAGATTATGTGCGACTGAT
  GAAGTATGGCGACTCTCTCTACCGCTGCAAACAGCTGAAGCGTGCGGCCCTGGGACGGATGTGCACAGTG
  ATCAAGAGGCAGAAGCAGAGTTTGGAGTATTTGGAGCAAGTGCGTCAGCATTTATCCCGTTTGCCAACCA
  TTGATCCGAATACCAGGACCCTGCTTTTGTGTGGGTACCCAAATGTTGGGAAGTCCAGCTTCATCAACAA
  GGTGACGAGAGCAGACGTGGATGTCCAGCCCTATGCGTTCACAACCAAGTCTCTGTTTGTTGGGCACATG
  GATTATAAGTATCTACGTTGGCAGGTTGTAGACACTCCTGGGATCCTGGACCACCCTCTGGAGGATAGGA
  ACACCATCGAGATGCAGGCCATCACTGCCCTGGCCCACCTCCGTGCTGCAGTCCTGTATGTGATGGATTT
  GTCTGAGCAGTGTGGGCATGGGCTGAGGGAGCAGCTAGAACTCTTCCAGAACATCAGACCTCTCTTCATC
  AACAAGCCTCTCATAGTTGTAGCCAACAAATGTGATGTGAAGAGAATAGCTGAACTTTCTGAAGATGATC
  AGAAAATATTTACAGATTTGCAGTCTGAAGGATTCCCTGTAATAGAGACCAGCACCCTGACTGAGGAAGG
  TGTTATTAAAGTTAAAACAGAGGCTTGCGATAGGCTTTTGGCTCATCGAGTGGAAACCAAAATGAAGGGA
  AATAAAGTGAATGAGGTGCTGAATAGACTGCACCTGGCTATCCCAACCAGGAGGGACGATAAGGAGAGGC
  CCCCTTTCATCCCTGAAGGAGTGGTGGCTCGCAGGAAGAGGATGGAAACTGAGGAGTCCAGGAAGAAGAG
  GGAACGAGATCTTGAGCTGGAAATGGGAGATGATTATATTTTGGATCTTCAGAAGTACTGGGATTTAATG
  AATTTGTCTGAAAAACATGATAAGATACCAGAAATCTGGGAAGGCCATAATATAGCTGATTATATTGATC
  CAGCCATCATGAAGAAATTGGAAGAATTAGAAAAAGAAGAAGAGCTGAGAACAGCTGCTGGAGAGTATGA
  CAGTGTATCTGAGAGTGAAGACGAAGAGATGCTGGAAATCCGACAGCTGGCAAAGCAAATTCGAGAGAAA
  AAGAAGTTGAAAATTCTGGAGTCCAAAGAAAAGAATACACAGGGACCCAGGATGCCGCGAACTGCTAAGA
  AGGTTCAGAGGACAGTTTTGGAGAAGGAGATGCGTAGTCTTGGTGTTGACATGGACGATAAAGACGATGC
  CCATTACGCAGTCCAGGCAAGAAGATCCCGGAGCATCACTAGGAAAAGAAAGCGGGAAGACTCTGCTCCC
  CCGTCCTCTGTGGCCCGGAGTGGGAGTTGCTCTCGAACTCCACGTGACGTTTCTGGTCTTAGGGATGTCA
  AGATGGTGAAGAAAGCCAAGACTATGATGAAGAATGCTCAGAAGAAGATGAATCGGTTGGGGAAGAAAGG
  GGAGGCGGATAGACACGTGTTTGATATGAAGCCCAAGCACTTGCTGTCTGGGAAGAGGAAAGCTGGTAAA
  AAGGACAGGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207903 representing NM_012341
  Red=Cloning site Green=Tags(s)
  
  MAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYQIHRIRHFYMRKVKFTQQNYHDRLSQILTDFP
  KLDDIHPFYADLMNILYDKDHYKLALGQINIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTV
  IKRQKQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHM
  DYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLREQLELFQNIRPLFI
  NKPLIVVANKCDVKRIAELSEDDQKIFTDLQSEGFPVIETSTLTEEGVIKVKTEACDRLLAHRVETKMKG
  NKVNEVLNRLHLAIPTRRDDKERPPFIPEGVVARRKRMETEESRKKRERDLELEMGDDYILDLQKYWDLM
  NLSEKHDKIPEIWEGHNIADYIDPAIMKKLEELEKEEELRTAAGEYDSVSESEDEEMLEIRQLAKQIREK
  KKLKILESKEKNTQGPRMPRTAKKVQRTVLEKEMRSLGVDMDDKDDAHYAVQARRSRSITRKRKREDSAP
  PSSVARSGSCSRTPRDVSGLRDVKMVKKAKTMMKNAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGK
  KDRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012341
• ORF Size: 1902 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012341.3
• RefSeq Size: 2537 bp
• RefSeq ORF: 1905 bp
• Locus ID: 23560
• UniProt ID: Q9BZE4
• Cytogenetics: 10p15.3
• Protein Families: Druggable Genome
• MW: 74 kDa
• Gene Summary: GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq, Jul 2008]