ZNF96 (ZSCAN12) (NM_001039643) Human Tagged ORF Clone

CAT#: RC207900

ZSCAN12 (Myc-DDK-tagged)-Human zinc finger and SCAN domain containing 12 (ZSCAN12)

Product Images

Circular map for RC207900

• 

  Western blot validation of overexpression lysate (Cat# LY422096) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207900 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF96
• Synonyms: dJ29K1.2; KIAA0426; ZFP96; ZFP96, ZNF305, ZNF29K1, ZSCAN12, KIAA0426, dJ29K1.2; zinc finger and SCAN domain containing 12; zinc finger protein 96; zinc finger protein 305; ZNF29K1; ZNF96; ZNF305
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207900 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTACTTGGGCTATCCAGGCCCACATGGACCAGGATGAACCTTTGGAAGTAAAGATAGAGGAAG
  AGAAATATACCACCAGACAGGATTGGGACCTGCGTAAAAACAACACCCATAGCAGAGAGGTCTTCCGTCA
  GTACTTCAGACAGTTCTGCTACCAGGAGACATCTGGTCCCCGTGAGGCTTTGAGCCGACTCCGAGAACTT
  TGCCATCAGTGGCTGAGGCCAGAGACCCACACCAAAGAACAGATTCTGGAGCTGCTGGTGCTGGAGCAGT
  TCCTGACCATCCTACCTGAGGAGCTCCAGGCCTGGGTGCAGGAGCAGCATCCAGAGAGTGGGGAGGAGGT
  GGTGACTGTGCTGGAGGATTTAGAGAGAGAACTGGATGAACCAGGAGAGCAGGTCTCAGTCCACACTGGG
  GAACAGGAAATGTTCTTGCAGGAGACGGTACGTCTACGAAAAGAAGGAGAACCCAGTATGTCCCTCCAGT
  CCATGAAAGCCCAGCCAAAGTATGAATCTCCAGAACTTGAATCCCAACAGGAGCAAGTTTTAGATGTTGA
  GACTGGAAATGAGTATGGGAATTTAAAGCAAGAAGTTTCTGAAGAAATGGAACCACATGGGAAGACATCC
  AGTAAATTTGAAAATGATATGTCCAAGTCTGCTAGGTGTGGAGAAACTCGTGAACCTGAAGAAATAACAG
  AAGAGCCCTCTGCTTGCTCCAGAGAAGATAAACAACCTACCTGTGATGAAAATGGAGTAAGCCTGACTGA
  GAACTCTGACCATACTGAACATCAGAGAATCTGTCCAGGAGAAGAATCTTACGGATGTGATGACTGTGGA
  AAAGCTTTTAGTCAGCACTCACACCTCATAGAACATCAGAGGATCCATACTGGAGATAGACCCTACAAAT
  GCGAAGAATGTGGAAAAGCTTTCCGTGGGAGAACTGTGCTTATTCGACACAAAATAATACACACTGGAGA
  GAAACCGTATAAGTGTAATGAATGTGGCAAAGCCTTTGGCCGGTGGTCAGCTCTTAACCAACATCAGAGA
  CTTCACACAGGAGAAAAACACTATCACTGTAATGACTGTGGCAAAGCCTTTAGTCAGAAAGCAGGCCTCT
  TTCACCATATCAAGATCCACACAAGAGACAAACCTTATCAGTGCACTCAGTGTAATAAAAGTTTTAGTCG
  GCGTTCCATACTTACTCAGCATCAAGGAGTTCATACCGGCGCCAAGCCGTATGAGTGCAACGAGTGTGGA
  AAAGCCTTTGTTTATAACTCATCCCTTGTTTCCCATCAGGAAATCCACCACAAAGAAAAATGCTATCAGT
  GTAAGGAATGTGGGAAATCCTTCAGTCAGAGTGGCCTTATTCAGCATCAGAGAATTCACACTGGGGAAAA
  ACCCTACAAATGTGACGTATGTGAAAAAGCCTTTATTCAAAGGACAAGTCTTACAGAACATCAGCGAATT
  CACACTGGAGAAAGACCCTATAAATGTGATAAGTGTGGGAAGGCGTTTACTCAAAGATCAGTCCTCACGG
  AACATCAGAGAATCCACACTGGAGAGAGGCCCTACAAGTGTGATGAGTGTGGGAATGCCTTCCGAGGAAT
  CACCAGCCTCATTCAGCATCAGAGAATCCACACTGGGGAGAAGCCCTACCAATGTGATGAGTGTGGAAAA
  GCCTTCAGACAGAGGAAGAAGACCAGCTACAAGGAAATACTTCTGAAAAATCACTCTGAACCCCAAGCTG
  GAGTGAATCTTCTCCTGAGCTCTCTCATTCCAGAATGGCAATCTTGTTTCAGGAGAGATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207900 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASTWAIQAHMDQDEPLEVKIEEEKYTTRQDWDLRKNNTHSREVFRQYFRQFCYQETSGPREALSRLREL
  CHQWLRPETHTKEQILELLVLEQFLTILPEELQAWVQEQHPESGEEVVTVLEDLERELDEPGEQVSVHTG
  EQEMFLQETVRLRKEGEPSMSLQSMKAQPKYESPELESQQEQVLDVETGNEYGNLKQEVSEEMEPHGKTS
  SKFENDMSKSARCGETREPEEITEEPSACSREDKQPTCDENGVSLTENSDHTEHQRICPGEESYGCDDCG
  KAFSQHSHLIEHQRIHTGDRPYKCEECGKAFRGRTVLIRHKIIHTGEKPYKCNECGKAFGRWSALNQHQR
  LHTGEKHYHCNDCGKAFSQKAGLFHHIKIHTRDKPYQCTQCNKSFSRRSILTQHQGVHTGAKPYECNECG
  KAFVYNSSLVSHQEIHHKEKCYQCKECGKSFSQSGLIQHQRIHTGEKPYKCDVCEKAFIQRTSLTEHQRI
  HTGERPYKCDKCGKAFTQRSVLTEHQRIHTGERPYKCDECGNAFRGITSLIQHQRIHTGEKPYQCDECGK
  AFRQRKKTSYKEILLKNHSEPQAGVNLLLSSLIPEWQSCFRRDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039643
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039643.1, NP_001034732.1
• RefSeq Size: 3931 bp
• RefSeq ORF: 1814 bp
• Locus ID: 9753
• Cytogenetics: 6p22.1
• Protein Families: Transcription Factors
• MW: 70.2 kDa