PDSS2 (NM_020381) Human Tagged ORF Clone

CAT#: RC207892

PDSS2 (Myc-DDK-tagged)-Human prenyl (decaprenyl) diphosphate synthase, subunit 2 (PDSS2)

Product Images

Circular map for RC207892

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDSS2 (Cat# RC207892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDSS2(Cat# TA503951). Positive lysates LY412512 (100ug) and LC412512 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412512) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207892 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDSS2 protein (Cat# TP307892). The protein was produced from HEK293T cells transfected with PDSS2 cDNA clone (Cat# RC207892) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDSS2
• Synonyms: bA59I9.3; C6orf210; COQ1B; COQ10D3; DLP1; hDLP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207892 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTCGGCAGCTGCTGTTGCACTTGCCACGTTATCTTGGAGCCTCGGGTTCCCCGCGTCGCCTGT
  GGTGGTCCCCGTCCCTCGACACCATCTCCTCGGTGGGCTCTTGGCGTGGTCGGTCCTCCAAGTCCCCGGC
  CCACTGGAATCAGGTAGTGTCAGAGGCGGAGAAGATCGTGGGGTACCCCACGTCCTTCATGAGCCTTCGC
  TGCCTGCTGAGCGACGAGCTCAGCAACATCGCTATGCAGGTGCGGAAGCTGGTGGGCACTCAGCACCCTC
  TGCTTACCACAGCCAGGGGGCTTGTACATGACAGCTGGAATAGCCTCCAGTTGAGGGGCTTGGTGGTGCT
  CCTTATCTCTAAAGCAGCTGGGCCCAGCAGCGTGAACACTTCATGTCAGAACTATGACATGGTCAGTGGG
  ATCTACTCATGTCAAAGAAGTTTGGCAGAGATCACGGAGCTAATTCATATTGCTCTCCTTGTACATCGTG
  GGATAGTAAATTTAAATGAGTTGCAATCATCTGATGGTCCACTGAAAGACATGCAATTTGGAAATAAAAT
  TGCTATCCTGAGTGGAGACTTTCTTCTAGCAAATGCCTGCAATGGACTAGCTCTGCTACAGAACACCAAG
  GTTGTGGAACTTTTAGCAAGTGCTCTTATGGACTTGGTACAAGGAGTATATCATGAAAATTCTACTTCAA
  AGGAAAGTTATATCACAGATGATATTGGAATATCGACTTGGAAGGAGCAGACTTTTCTCTCCCATGGTGC
  CTTACTAGCAAAGAGCTGCCAAGCTGCAATGGAATTAGCAAAGCATGATGCTGAGGTTCAGAATATGGCA
  TTTCAGTATGGGAAGCACATGGCCATGAGTCATAAGATAAATTCTGATGTCCAGCCTTTTATTAAAGAAA
  AGACCAGTGACTCCATGACTTTTAATCTAAACTCAGCTCCTGTAGTCTTACATCAGGAATTTCTTGGAAG
  AGATTTGTGGATTAAACAGATCAGAGAGGCTCAAGAAAAAGGAAGATTGGACTATGCTAAGTTGCGAGAA
  AGAATCAAAGCTGGCAAAGGTGTGACTTCAGCTATTGACCTGTGTCGTTACCATGGAAACAAGGCACTGG
  AGGCCCTGGAGAGCTTTCCTCCCTCGGAGGCCAGATCTGCTTTAGAAAACATTGTGTTTGCTGTGACCAG
  ATTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207892 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFRQLLLHLPRYLGASGSPRRLWWSPSLDTISSVGSWRGRSSKSPAHWNQVVSEAEKIVGYPTSFMSLR
  CLLSDELSNIAMQVRKLVGTQHPLLTTARGLVHDSWNSLQLRGLVVLLISKAAGPSSVNTSCQNYDMVSG
  IYSCQRSLAEITELIHIALLVHRGIVNLNELQSSDGPLKDMQFGNKIAILSGDFLLANACNGLALLQNTK
  VVELLASALMDLVQGVYHENSTSKESYITDDIGISTWKEQTFLSHGALLAKSCQAAMELAKHDAEVQNMA
  FQYGKHMAMSHKINSDVQPFIKEKTSDSMTFNLNSAPVVLHQEFLGRDLWIKQIREAQEKGRLDYAKLRE
  RIKAGKGVTSAIDLCRYHGNKALEALESFPPSEARSALENIVFAVTRFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020381
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020381.2, NP_065114.2
• RefSeq Size: 3568 bp
• RefSeq ORF: 1200 bp
• Locus ID: 57107
• UniProt ID: Q86YH6
• Cytogenetics: 6q21
• Protein Pathways: Terpenoid backbone biosynthesis
• MW: 44.2 kDa
• Gene Summary: The protein encoded by this gene is an enzyme that synthesizes the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. Defects in this gene are a cause of coenzyme Q10 deficiency.[provided by RefSeq, Oct 2009]