FRMD8 (NM_031904) Human Tagged ORF Clone

CAT#: RC207879

FRMD8 (Myc-DDK-tagged)-Human FERM domain containing 8 (FRMD8)

Product Images

Circular map for RC207879

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  Western blot validation of overexpression lysate (Cat# LY410451) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207879 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FRMD8 protein (Cat# TP307879). The protein was produced from HEK293T cells transfected with FRMD8 cDNA clone (Cat# RC207879) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FRMD8
• Synonyms: FKSG44; iTAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207879 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGAAGCAGCGTGTCCT
  CCGTGGGAGCCCGAGCGGCTGACGTGCTGGTATACCTAGCGGATGACACGGTGGTGCCCCTGGCTGTGGA
  GAACCTGCCCTCGCTCAGTGCCCATGAGCTGCACCGCGCTGTCCGCGAGGTCCTGCAGCTTCCAGACATC
  GCCCTGGATGTCTTCGCGCTCTGGCTGGTCTCCCCTCTGCTGGAGGTGCAGCTGAAACCCAAGCACCAGC
  CCTACAAGCTGGGACGCCAGTGGCCGGAGCTGCTGCTGCGCTTCACCAGTGCCCCAGATGATGACGTGGC
  CATGGATGAGCCTTTCCTGCAGTTCCGAAGGAACGTGTTCTTCCCAAAGCGGCGGGAGCTCCAGATCCAT
  GACGAGGAGGTCCTGCGGCTGCTCTATGAGGAGGCCAAGGGCAACGTGCTGGCTGCACGGTACCCGTGCG
  ACGTGGAGGACTGCGAGGCTCTGGGCGCCCTGGTGTGCCGCGTGCAGCTTGGGCCCTACCAGCCCGGCCG
  GCCGGCAGCCTGCGACCTGAGGGAGAAGCTGGACTCCTTCCTCCCTGCCCACCTCTGTAAGCGGGGCCAG
  AGTCTCTTTGCTGCCCTCCGGGGCCGTGGGGCCAGGGCCGGGCCGGGCGAGCAGGGCCTGCTGAACGCCT
  ACCGCCAGGTGCAGGAGGTCAGCAGCGACGGCGGGTGCGAGGCCGCCCTGGGCACCCACTACCGCGCCTA
  TCTCCTCAAGTGCCACGAGCTGCCGTTTTATGGGTGTGCCTTCTTCCACGGTGAGGTTGACAAGCCGGCC
  CAAGGCTTTTTGCACCGGGGTGGGCGCAAGCCAGTTTCTGTGGCCATCAGTCTGGAAGGCGTGCACGTCA
  TCGATAGCAGAGAGAAGCATGTCCTGCTGGGCCTGCGCTTCCAGGAGCTGTCGTGGGACCACACCTCCCC
  CGAGGAGGAGGAGCCCATCTTGTGGCTGGAGTTCGACGGGGACAGCGAGGGCACACCTGTCAACAAGCTC
  CTCAAGATCTACTCCAAGCAGGCCGAACTGATGAGCAGTCTCATTGAGTACTGCATCGAACTGAGCCAGG
  CGGCGGAGCCCGCAGGCCCCCAGGACAGTGCGACTGGCTCGCCCTCGGACCCCAGCTCCTCACTGGCTCC
  TGTTCAGCGCCCCAAGCTGCGGAGGCAGGGCAGTGTGGTGTCCAGCCGGATCCAGCATCTCTCCACCATC
  GACTACGTGGAGGACGGCAAGGGGATCAGGCGAGTGAAGCCGAAGCGCACCACATCCTTCTTCAGCCGGC
  AGCTGTCCTTGGGCCAGGGGAGCTACACCGTGGTGCAGCCCGGCGACAGCCTGGAGCAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207879 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDGTEGSAGQPGPAERSHRSSVSSVGARAADVLVYLADDTVVPLAVENLPSLSAHELHRAVREVLQLPDI
  ALDVFALWLVSPLLEVQLKPKHQPYKLGRQWPELLLRFTSAPDDDVAMDEPFLQFRRNVFFPKRRELQIH
  DEEVLRLLYEEAKGNVLAARYPCDVEDCEALGALVCRVQLGPYQPGRPAACDLREKLDSFLPAHLCKRGQ
  SLFAALRGRGARAGPGEQGLLNAYRQVQEVSSDGGCEAALGTHYRAYLLKCHELPFYGCAFFHGEVDKPA
  QGFLHRGGRKPVSVAISLEGVHVIDSREKHVLLGLRFQELSWDHTSPEEEEPILWLEFDGDSEGTPVNKL
  LKIYSKQAELMSSLIEYCIELSQAAEPAGPQDSATGSPSDPSSSLAPVQRPKLRRQGSVVSSRIQHLSTI
  DYVEDGKGIRRVKPKRTTSFFSRQLSLGQGSYTVVQPGDSLEQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031904
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031904.2, NP_114110.1
• RefSeq Size: 3774 bp
• RefSeq ORF: 1395 bp
• Locus ID: 83786
• UniProt ID: Q9BZ67
• Cytogenetics: 11q13.1
• Domains: B41
• MW: 51.2 kDa
• Gene Summary: Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway. By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (PubMed:29897333, PubMed:29897336). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (PubMed:19572019).[UniProtKB/Swiss-Prot Function]