FASTK (NM_033015) Human Tagged ORF Clone

CAT#: RC207877

FASTK (Myc-DDK-tagged)-Human Fas-activated serine/threonine kinase (FASTK), transcript variant 4

Product Images

Circular map for RC207877

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  Western blot validation of overexpression lysate (Cat# LY409778) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207877 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FASTK protein (Cat# TP307877). The protein was produced from HEK293T cells transfected with FASTK cDNA clone (Cat# RC207877) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FASTK
• Synonyms: FAST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207877 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGC
  CCGGGGAGTCATGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCT
  CCCTCCGAAGCCACCTCCCCCTTTGCAGCCCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGC
  TGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAGGCCAGAGGAAC
  TGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCT
  GGAAGCCATTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTC
  CTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGG
  CTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCT
  GCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCT
  CTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTC
  CCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCAA
  GTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGAC
  CTGACTGTGCCTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCG
  TGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAGCGC
  CACTACTCGAGACCCTGCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGC
  CGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGC
  TACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCA
  GGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207877 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRPRGEPGPRAPRPTEGATCAGPGESCFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAALS
  CPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLV
  LPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHA
  LIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQD
  LTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDG
  RVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033015
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033015.3
• RefSeq Size: 1448 bp
• RefSeq ORF: 1227 bp
• Locus ID: 10922
• UniProt ID: Q14296
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 45.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]