CLCA2 (NM_006536) Human Tagged ORF Clone

CAT#: RC207731

CLCA2 (Myc-DDK-tagged)-Human chloride channel accessory 2 (CLCA2)

Product Images

Circular map for RC207731

• 

  Western blot validation of overexpression lysate (Cat# LY416578) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207731 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CLCA2 protein (Cat# TP307731). The protein was produced from HEK293T cells transfected with CLCA2 cDNA clone (Cat# RC207731) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLCA2
• Synonyms: CACC; CaCC-3; CACC3; CLCRG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207731 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAAAGGAGCATTGCAGGTCCTATTTGCAACCTGAAGTTTGTGACTCTCCTGGTTGCCTTAAGTT
  CAGAACTCCCATTCCTGGGAGCTGGAGTACAGCTTCAAGACAATGGGTATAATGGATTGCTCATTGCAAT
  TAATCCTCAGGTACCTGAGAATCAGAACCTCATCTCAAACATTAAGGAAATGATAACTGAAGCTTCATTT
  TACCTATTTAATGCTACCAAGAGAAGAGTATTTTTCAGAAATATAAAGATTTTAATACCTGCCACATGGA
  AAGCTAATAATAACAGCAAAATAAAACAAGAATCATATGAAAAGGCAAATGTCATAGTGACTGACTGGTA
  TGGGGCACATGGAGATGATCCATACACCCTACAATACAGAGGGTGTGGAAAAGAGGGAAAATACATTCAT
  TTCACACCTAATTTCCTACTGAATGATAACTTAACAGCTGGCTACGGATCACGAGGCCGAGTGTTTGTCC
  ATGAATGGGCCCACCTCCGTTGGGGTGTGTTCGATGAGTATAACAATGACAAACCTTTCTACATAAATGG
  GCAAAATCAAATTAAAGTGACAAGGTGTTCATCTGACATCACAGGCATTTTTGTGTGTGAAAAAGGTCCT
  TGCCCCCAAGAAAACTGTATTATTAGTAAGCTTTTTAAAGAAGGATGCACCTTTATCTACAATAGCACCC
  AAAATGCAACTGCATCAATAATGTTCATGCAAAGTTTATCTTCTGTGGTTGAATTTTGTAATGCAAGTAC
  CCACAACCAAGAAGCACCAAACCTACAGAACCAGATGTGCAGCCTCAGAAGTGCATGGGATGTAATCACA
  GACTCTGCTGACTTTCACCACAGCTTTCCCATGAATGGGACTGAGCTTCCACCTCCTCCCACATTCTCGC
  TTGTACAGGCTGGTGACAAAGTGGTCTGTTTAGTGCTGGATGTGTCCAGCAAGATGGCAGAGGCTGACAG
  ACTCCTTCAACTACAACAAGCCGCAGAATTTTATTTGATGCAGATTGTTGAAATTCATACCTTCGTGGGC
  ATTGCCAGTTTCGACAGCAAAGGAGAGATCAGAGCCCAGCTACACCAAATTAACAGCAATGATGATCGAA
  AGTTGCTGGTTTCATATCTGCCCACCACTGTATCAGCTAAAACAGACATCAGCATTTGTTCAGGGCTTAA
  GAAAGGATTTGAGGTGGTTGAAAAACTGAATGGAAAAGCTTATGGCTCTGTGATGATATTAGTGACCAGC
  GGAGATGATAAGCTTCTTGGCAATTGCTTACCCACTGTGCTCAGCAGTGGTTCAACAATTCACTCCATTG
  CCCTGGGTTCATCTGCAGCCCCAAATCTGGAGGAATTATCACGTCTTACAGGAGGTTTAAAGTTCTTTGT
  TCCAGATATATCAAACTCCAATAGCATGATTGATGCTTTCAGTAGAATTTCCTCTGGAACTGGAGACATT
  TTCCAGCAACATATTCAGCTTGAAAGTACAGGTGAAAATGTCAAACCTCACCATCAATTGAAAAACACAG
  TGACTGTGGATAATACTGTGGGCAACGACACTATGTTTCTAGTTACGTGGCAGGCCAGTGGTCCTCCTGA
  GATTATATTATTTGATCCTGATGGACGAAAATACTACACAAATAATTTTATCACCAATCTAACTTTTCGG
  ACAGCTAGTCTTTGGATTCCAGGAACAGCTAAGCCTGGGCACTGGACTTACACCCTGAACAATACCCATC
  ATTCTCTGCAAGCCCTGAAAGTGACAGTGACCTCTCGCGCCTCCAACTCAGCTGTGCCCCCAGCCACTGT
  GGAAGCCTTTGTGGAAAGAGACAGCCTCCATTTTCCTCATCCTGTGATGATTTATGCCAATGTGAAACAG
  GGATTTTATCCCATTCTTAATGCCACTGTCACTGCCACAGTTGAGCCAGAGACTGGAGATCCTGTTACGC
  TGAGACTCCTTGATGATGGAGCAGGTGCTGATGTTATAAAAAATGATGGAATTTACTCGAGGTATTTTTT
  CTCCTTTGCTGCAAATGGTAGATATAGCTTGAAAGTGCATGTCAATCACTCTCCCAGCATAAGCACCCCA
  GCCCACTCTATTCCAGGGAGTCATGCTATGTATGTACCAGGTTACACAGCAAACGGTAATATTCAGATGA
  ATGCTCCAAGGAAATCAGTAGGCAGAAATGAGGAGGAGCGAAAGTGGGGCTTTAGCCGAGTCAGCTCAGG
  AGGCTCCTTTTCAGTGCTGGGAGTTCCAGCTGGCCCCCACCCTGATGTGTTTCCACCATGCAAAATTATT
  GACCTGGAAGCTGTAAAAGTAGAAGAGGAATTGACCCTATCTTGGACAGCACCTGGAGAAGACTTTGATC
  AGGGCCAGGCTACAAGCTATGAAATAAGAATGAGTAAAAGTCTACAGAATATCCAAGATGACTTTAACAA
  TGCTATTTTAGTAAATACATCAAAGCGAAATCCTCAGCAAGCTGGCATCAGGGAGATATTTACGTTCTCA
  CCCCAAATTTCCACGAATGGACCTGAACATCAGCCAAATGGAGAAACACATGAAAGCCACAGAATTTATG
  TTGCAATACGAGCAATGGATAGGAACTCCTTACAGTCTGCTGTATCTAACATTGCCCAGGCGCCTCTGTT
  TATTCCCCCCAATTCTGATCCTGTACCTGCCAGAGATTATCTTATATTGAAAGGAGTTTTAACAGCAATG
  GGTTTGATAGGAATCATTTGCCTTATTATAGTTGTGACACATCATACTTTAAGCAGGAAAAAGAGAGCAG
  ACAAGAAAGAGAATGGAACAAAATTATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207731 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTQRSIAGPICNLKFVTLLVALSSELPFLGAGVQLQDNGYNGLLIAINPQVPENQNLISNIKEMITEASF
  YLFNATKRRVFFRNIKILIPATWKANNNSKIKQESYEKANVIVTDWYGAHGDDPYTLQYRGCGKEGKYIH
  FTPNFLLNDNLTAGYGSRGRVFVHEWAHLRWGVFDEYNNDKPFYINGQNQIKVTRCSSDITGIFVCEKGP
  CPQENCIISKLFKEGCTFIYNSTQNATASIMFMQSLSSVVEFCNASTHNQEAPNLQNQMCSLRSAWDVIT
  DSADFHHSFPMNGTELPPPPTFSLVQAGDKVVCLVLDVSSKMAEADRLLQLQQAAEFYLMQIVEIHTFVG
  IASFDSKGEIRAQLHQINSNDDRKLLVSYLPTTVSAKTDISICSGLKKGFEVVEKLNGKAYGSVMILVTS
  GDDKLLGNCLPTVLSSGSTIHSIALGSSAAPNLEELSRLTGGLKFFVPDISNSNSMIDAFSRISSGTGDI
  FQQHIQLESTGENVKPHHQLKNTVTVDNTVGNDTMFLVTWQASGPPEIILFDPDGRKYYTNNFITNLTFR
  TASLWIPGTAKPGHWTYTLNNTHHSLQALKVTVTSRASNSAVPPATVEAFVERDSLHFPHPVMIYANVKQ
  GFYPILNATVTATVEPETGDPVTLRLLDDGAGADVIKNDGIYSRYFFSFAANGRYSLKVHVNHSPSISTP
  AHSIPGSHAMYVPGYTANGNIQMNAPRKSVGRNEEERKWGFSRVSSGGSFSVLGVPAGPHPDVFPPCKII
  DLEAVKVEEELTLSWTAPGEDFDQGQATSYEIRMSKSLQNIQDDFNNAILVNTSKRNPQQAGIREIFTFS
  PQISTNGPEHQPNGETHESHRIYVAIRAMDRNSLQSAVSNIAQAPLFIPPNSDPVPARDYLILKGVLTAM
  GLIGIICLIIVVTHHTLSRKKRADKKENGTKLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006536
• ORF Size: 2829 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006536.7
• RefSeq Size: 4043 bp
• RefSeq ORF: 2832 bp
• Locus ID: 9635
• UniProt ID: Q9UQC9
• Cytogenetics: 1p22.3
• Domains: VWA
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 103.9 kDa
• Gene Summary: This gene encodes a member of the calcium-activated chloride channel regulator (CLCR) family of proteins. Members of this family regulate the transport of chloride across the plasma membrane. The encoded protein is autoproteolytically processed to generate N- and C- terminal fragments. Expression of this gene is upregulated by the tumor suppressor protein p53 in response to DNA damage. In breast cancer, expression of this gene is downregulated and the encoded protein may inhibit migration and invasion while promoting mesenchymal-to-epithelial transition in cancer cell lines. [provided by RefSeq, Sep 2016]