NUDT12 (NM_031438) Human Tagged ORF Clone

CAT#: RC207724

NUDT12 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 12 (NUDT12)

Product Images

Circular map for RC207724

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUDT12 (Cat# RC207724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUDT12(Cat# TA805571). Positive lysates LY410513 (100ug) and LC410513 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410513) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207724 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NUDT12 protein (Cat# TP307724). The protein was produced from HEK293T cells transfected with NUDT12 cDNA clone (Cat# RC207724) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUDT12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207724 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCTGTAAAAAGAAGTCTGAAGCAAGAAATAGTTACTCAGTTTCACTGTTCAGCTGCTGAAGGAG
  ATATTGCCAAGTTAACAGGAATACTCAGTCATTCTCCATCTCTTCTCAATGAAACTTCTGAAAATGGCTG
  GACTGCTTTAATGTATGCGGCAAGGAATGGGCACCCAGAGATAGTCCAATTTCTGCTTGAGAAAGGGTGT
  GACAGATCAATTGTCAATAAATCAAGGCAGACTGCACTGGATATTGCTGTATTTTGGGGTTATAAGCATA
  TAGCTAATTTACTAGCTACTGCTAAAGGTGGGAAGAAGCCTTGGTTCCTAACGAATGAAGTGGAAGAATG
  TGAAAATTATTTTAGCAAAACACTACTGGACCGGAAAAGTGAAAAGAGGAATAATTCTGACTGGCTGCTA
  GCTAAAGAAAGCCATCCAGCCACAGTTTTTATTCTTTTCTCAGATTTAAATCCCTTGGTTACTCTAGGTG
  GCAATAAAGAAAGTTTCCAACAGCCAGAAGTTAGGCTTTGTCAGCTGAACTACACAGATATAAAGGATTA
  TTTGGCCCAGCCTGAGAAGATCACCTTGATTTTTCTTGGAGTAGAACTTGAAATAAAAGACAAACTACTT
  AATTATGCTGGTGAAGTCCCGAGAGAGGAGGAAGATGGATTGGTTGCCTGGTTTGCTCTAGGTATAGATC
  CTATTGCTGCTGAAGAATTCAAGCAAAGACATGAAAATTGTTACTTTCTTCATCCTCCTATGCCAGCCCT
  TCTGCAATTGAAAGAAAAAGAAGCTGGGGTTGTAGCTCAAGCAAGATCTGTTCTTGCCTGGCACAGTCGA
  TACAAGTTTTGCCCAACCTGTGGAAATGCAACTAAAATTGAAGAAGGTGGCTATAAGAGACTATGTTTAA
  AAGAAGACTGTCCTAGTCTCAATGGCGTCCATAATACCTCATACCCAAGAGTTGATCCAGTAGTAATCAT
  GCAAGTTATTCATCCAGATGGGACCAAATGCCTTTTAGGCAGGCAGAAAAGATTTCCCCCAGGCATGTTT
  ACTTGCCTTGCTGGATTTATTGAGCCTGGAGAGACAATAGAAGATGCTGTTAGGAGAGAAGTAGAAGAGG
  AAAGTGGAGTCAAAGTTGGCCATGTTCAGTATGTTGCTTGTCAACCATGGCCAATGCCTTCCTCCTTAAT
  GATTGGTTGCTTAGCTCTAGCAGTGTCTACAGAAATTAAAGTTGACAAGAATGAAATAGAGGATGCCCGC
  TGGTTCACTAGAGAACAGGTCCTGGATGTTCTGACCAAAGGGAAGCAGCAGGCATTCTTTGTGCCACCAA
  GCCGAGCTATTGCACATCAATTAATCAAACACTGGATTAGAATAAATCCTAATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207724 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSVKRSLKQEIVTQFHCSAAEGDIAKLTGILSHSPSLLNETSENGWTALMYAARNGHPEIVQFLLEKGC
  DRSIVNKSRQTALDIAVFWGYKHIANLLATAKGGKKPWFLTNEVEECENYFSKTLLDRKSEKRNNSDWLL
  AKESHPATVFILFSDLNPLVTLGGNKESFQQPEVRLCQLNYTDIKDYLAQPEKITLIFLGVELEIKDKLL
  NYAGEVPREEEDGLVAWFALGIDPIAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSR
  YKFCPTCGNATKIEEGGYKRLCLKEDCPSLNGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMF
  TCLAGFIEPGETIEDAVRREVEEESGVKVGHVQYVACQPWPMPSSLMIGCLALAVSTEIKVDKNEIEDAR
  WFTREQVLDVLTKGKQQAFFVPPSRAIAHQLIKHWIRINPNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031438
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031438.1
• RefSeq Size: 3514 bp
• RefSeq ORF: 1389 bp
• Locus ID: 83594
• UniProt ID: Q9BQG2
• Cytogenetics: 5q21.2
• Domains: NUDIX, ANK
• Protein Pathways: Nicotinate and nicotinamide metabolism
• MW: 52.1 kDa
• Gene Summary: Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12, regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al., 2003 [PubMed 12790796]).[supplied by OMIM, Mar 2008]