PCDH1 (NM_002587) Human Tagged ORF Clone

CAT#: RC207717

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PCDH1 (Myc-DDK-tagged)-Human protocadherin 1 (PCDH1), transcript variant 1



  "NM_002587" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,258.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PCDH1
Synonyms PC42; PCDH42
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207717 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAGCGGGGCGGGCGGCCGGCGCTGCCCGGAGGCGGCCCTCCTGATTCTGGGGCCTCCCAGGATGG
AGCACCTGAGGCACAGCCCAGGCCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCT
GCTCCTGCTGGCTCCATCCCCAGGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCC
AACACCCTCATTGGGAGCCTCGCAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGG
TGGGTGCCCCGTACCTTCGCGTGGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCG
TGAGGGGCTCCGTGAATGCCAGAACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATC
ACAGACCTCGTGCAGAATGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACA
ACACACCCAACTTCGCCTCACCAGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTT
CCCCATCCCGCTGGCTTCAGACCGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGG
CCTGAGGCCCAGGAGCTATTTGGGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTG
TGATGGGCAACCTGGACCGTGAGCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAG
CCCCCCACGCGCCAGCAGTGCCCTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTT
GAGCGGCCCTCCTATGAGGCCGAACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGG
CCAATGACTCAGACCAAGGTGCCAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAG
GCGTCTTCTTCGACTGGACAGGAACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTA
AGCACCCTGCGCTTCTCAGTGCTTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGG
TTGTGACCGTGAAGGACATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCA
TCAAGATGGGATGGCTAACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCT
GACCGAGATGAGGGAGAGAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCC
AGGCCAGTGAGACAGGCAGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGA
GAAGGTCAAAGACTACACCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAAC
TCCCTCAAGGTGCAGGTGGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCG
CCTTCCCGGAAAACAACAAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTC
TAATGCTGAGCTGGTTTACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAG
ACTGGAGAGATCCAGGTGAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGG
CAGCTGACCGGGGCAGTCCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGA
CAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCA
GTGGGCATGGTGACTGTCATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGG
ACAACGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCA
ACAAAGCACCTACACCTTCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGT
GTCACCATCAATGTGCTGGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACA
AGCTGCTGACCCCCCAGACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGG
TGTCAATGCCGAGCTGATCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACAT
TCAGGTGCCATCACCCTGGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGG
TCAGTGACCGCGGCAAGCCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGC
CAACCGCACGCTGCTGGAGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGG
GATCCAGAATATGAGCGCTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCG
TGGCCTTGCTCATCGCCCTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCA
GGCTGGTAAGAAGGAGACCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAA
AGCAAAGGCAAGAAGAGCAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGC
AGAAGTCCCTCAAGTTCAACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAA
CTACCCACCAGGCAGCCCTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTG
CAGCCCCAGTCACCCTCAGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCG
TGGGCACCGGGGACACCACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCC
CAAATACCCCAGCAAGCAGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTAC
CACGGAGCCATCTGGACCGAGGTGTGGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207717 protein sequence
Red=Cloning site Green=Tags(s)

MDSGAGGRRCPEAALLILGPPRMEHLRHSPGPGGQRLLLPSMLLALLLLLAPSPGHATRVVYKVPEEQPP
NTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSI
TDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASDRDAGPNGVASYELQAG
PEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKF
ERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDL
STLRFSVLAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVS
DRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTN
SLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGSNAELVYSLEPEPAAKGLFTISPE
TGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSP
VGMVTVIDGDKGENAQVQLSVEQDNGDFVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVG
VTINVLDENDNAPYITAPSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSH
SGAITLEKEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDIDIAG
DPEYERSKQRGNILFGVVAGVVAVALLIALAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKASKGNK
SKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQL
QPQSPSASKKHQVVQDLPPANTFVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQVGQPFQLSTPQPLPHPY
HGAIWTEVWE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002587
ORF Size 3180 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002587.5
RefSeq Size 3845 bp
RefSeq ORF 3183 bp
Locus ID 5097
UniProt ID Q08174
Cytogenetics 5q31.3
Domains CA
Protein Families Transcription Factors, Transmembrane
MW 114.7 kDa
Gene Summary This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008]

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