PCDH1 (NM_002587) Human Untagged Clone

CAT#: SC109553

PCDH1 (untagged)-Human protocadherin 1 (PCDH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDH1
• Synonyms: PC42; PCDH42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC109553 representing NM_002587
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCGGGGCGGGCGGCCGGCGCTGCCCGGAGGCGGCCCTCCTGATTCTGGGGCCTCCCAGGATGG
  AGCACCTGAGGCACAGCCCAGGCCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCT
  GCTCCTGCTGGCTCCATCCCCAGGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCC
  AACACCCTCATTGGGAGCCTCGCAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGG
  TGGGTGCCCCGTACCTTCGCGTGGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCG
  TGAGGGGCTCCGTGAATGCCAGAACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATC
  ACAGACCTCGTGCAGAATGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACA
  ACACACCCAACTTCGCCTCACCAGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTT
  CCCCATCCCGCTGGCTTCAGACCGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGG
  CCTGAGGCCCAGGAGCTATTTGGGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTG
  TGATGGGCAACCTGGACCGTGAGCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAG
  CCCCCCACGCGCCAGCAGTGCCCTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTT
  GAGCGGCCCTCCTATGAGGCCGAACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGG
  CCAATGACTCAGACCAAGGTGCCAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAG
  GCGTCTTCTTCGACTGGACAGGAACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTA
  AGCACCCTGCGCTTCTCAGTGCTTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGG
  TTGTGACCGTGAAGGACATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCA
  TCAAGATGGGATGGCTAACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCT
  GACCGAGATGAGGGAGAGAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCC
  AGGCCAGTGAGACAGGCAGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGA
  GAAGGTCAAAGACTACACCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAAC
  TCCCTCAAGGTGCAGGTGGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCG
  CCTTCCCGGAAAACAACAAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTC
  TAATGCTGAGCTGGTTTACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAG
  ACTGGAGAGATCCAGGTGAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGG
  CAGCTGACCGGGGCAGTCCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGA
  CAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCA
  GTGGGCATGGTGACTGTCATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGG
  ACAACGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCA
  ACAAAGCACCTACACCTTCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGT
  GTCACCATCAATGTGCTGGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACA
  AGCTGCTGACCCCCCAGACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGG
  TGTCAATGCTGAGCTGATCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACAT
  TCAGGTGCCATCACCCTGGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGG
  TCAGTGACCGCGGCAAGCCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGC
  CAACCGCACGCTGCTGGAGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGG
  GATCCAGAATATGAGCGCTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCG
  TGGCCTTGCTCATCGCCCTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCA
  GGCTGGTAAGAAGGAGACCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAA
  AGCAAAGGCAAGAAGAGCAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGC
  AGAAGTCCCTCAAGTTCAACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAA
  CTACCCACCAGGCAGCCCTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTG
  CAGCCCCAGTCACCCTCAGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCG
  TGGGCACCGGGGACACCACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCC
  CAAATACCCCAGCAAGCAGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTAC
  CACGGAGCCATCTGGACCGAGGTGTGGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: NotI-NotI
• ACCN: NM_002587
• Insert Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002587.3, NP_002578.2
• RefSeq Size: 3851 bp
• RefSeq ORF: 3183 bp
• Locus ID: 5097
• UniProt ID: Q08174
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) lacks two exons and its transcription extends past a splice site that is used in variant 2, resulting in a novel 3' coding region and 3' UTR compared to variant 2. It encodes isoform 1 which is shorter and has a distinct C-terminus, compared to isoform 2.