FHOD1 (NM_013241) Human Tagged ORF Clone

CAT#: RC207683

FHOD1 (Myc-DDK-tagged)-Human formin homology 2 domain containing 1 (FHOD1)

Product Images

Circular map for RC207683

• 

  Western blot validation of overexpression lysate (Cat# LY415719) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207683 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FHOD1 protein (Cat# TP307683). The protein was produced from HEK293T cells transfected with FHOD1 cDNA clone (Cat# RC207683) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FHOD1
• Synonyms: FHOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207683 representing NM_013241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGGGGAAGACCGCGGGGACGGAGAGCCGGTATCAGTGGTGACCGTGAGGGTGCAGTACCTGG
  AAGACACCGACCCCTTCGCATGTGCCAACTTTCCGGAGCCGCGCCGGGCCCCCACCTGCAGCCTGGACGG
  GGCGCTGCCCTTGGGCGCGCAGATACCCGCGGTGCACCGCCTGCTGGGAGCGCCGCTCAAGTTGGAGGAT
  TGTGCTCTGCAAGTGTCTCCCTCCGGATACTACCTGGACACCGAGCTGTCCCTGGAAGAGCAGCGGGAGA
  TGCTGGAGGGCTTCTATGAAGAGATCAGCAAAGGGCGGAAGCCCACGCTGATCCTTCGGACCCAGCTCTC
  TGTGAGGGTCAACGCTATCTTGGAAAAGCTGTATAGCTCCAGTGGTCCTGAGCTCCGCCGCTCCCTCTTC
  TCACTGAAGCAGATCTTCCAGGAGGACAAAGACCTGGTGCCTGAATTTGTGCATTCAGAGGGGCTGAGCT
  GCCTGATCCGTGTGGGTGCTGCTGCCGACCACAACTACCAGAGCTACATCCTTAGAGCGCTCGGCCAGCT
  GATGCTCTTTGTGGATGGAATGCTGGGGGTGGTGGCCCACAGTGACACTATTCAGTGGCTGTACACATTG
  TGTGCCAGCCTGTCCCGCTTGGTGGTGAAGACAGCCCTGAAGCTGCTGTTGGTGTTTGTAGAATACTCCG
  AAAACAACGCACCGCTGTTCATCCGTGCAGTGAACTCTGTGGCCAGCACCACCGGTGCTCCTCCCTGGGC
  CAATCTGGTGTCCATCCTGGAGGAGAAGAATGGCGCTGACCCTGAGTTGTTGGTGTACACGGTCACCCTC
  ATCAACAAGACGCTGGCGGCGCTCCCGGACCAGGACTCCTTCTACGATGTGACGGATGCACTGGAGCAGC
  AGGGCATGGAAGCGCTGGTCCAGCGCCACCTGGGCACTGCGGGCACTGACGTCGACCTGCGCACGCAGCT
  TGTGCTCTACGAGAACGCCCTGAAATTGGAGGATGGAGACATCGAAGAAGCCCCAGGCGCTGGTGGGCGG
  CGGGAACGACGAAAGCCTTCTTCTGAGGAGGGCAAGAGGAGCCGCCGTTCTCTGGAAGGCGGGGGCTGCC
  CCGCGCGTGCCCCGGAACCTGGCCCCACAGGCCCCGCCTCACCGGTAGGCCCCACCTCTTCCACCGGCCC
  CGCCCTGCTGACAGGCCCCGCCTCCAGCCCTGTGGGCCCTCCCTCCGGTCTCCAAGCTTCAGTGAACCTT
  TTTCCTACCATCTCTGTGGCACCCTCAGCTGACACCTCCAGCGAGAGGAGCATCTACAAAGCCCGGTTCC
  TGGAGAATGTGGCGGCAGCAGAAACAGAGAAGCAGGTTGCGCTGGCCCAGGGCCGGGCAGAGACACTTGC
  CGGGGCCATGCCCAATGAGGCGGGTGGACACCCAGATGCCCGGCAACTCTGGGACTCCCCAGAGACAGCC
  CCTGCAGCCAGAACACCCCAGAGCCCTGCCCCCTGTGTCCTGCTCCGGGCCCAGCGAAGCCTTGCACCAG
  AGCCCAAGGAGCCACTGATACCAGCAAGCCCCAAGGCTGAGCCCATCTGGGAGCTCCCTACCCGTGCACC
  CAGGCTCTCTATTGGGGACCTGGACTTTTCAGATCTAGGGGAGGATGAAGACCAGGACATGCTGAATGTA
  GAGTCTGTGGAGGCTGGGAAAGACATCCCAGCTCCCTCACCCCCACTGCCCCTGCTCTCGGGAGTACCCC
  CCCCTCCCCCACTTCCACCTCCCCCACCCATCAAAGGCCCCTTCCCACCACCTCCACCTCTACCTCTGGC
  TGCCCCTCTTCCCCATTCAGTGCCTGACAGCTCAGCCCTCCCCACTAAGAGGAAGACAGTAAAACTTTTC
  TGGCGTGAGCTGAAGCTGGCTGGGGGCCATGGAGTCTCTGCAAGCCGCTTTGGGCCCTGCGCCACCCTCT
  GGGCTTCACTGGACCCTGTCTCAGTGGACACGGCCCGACTGGAACACCTCTTTGAGTCTCGTGCCAAAGA
  GGTGCTGCCCTCCAAGAAAGCTGGAGAGGGCCGCCGGACAATGACCACAGTGCTGGACCCCAAGCGCAGC
  AACGCCATCAACATCGGCCTAACCACACTGCCACCTGTGCATGTCATTAAGGCTGCTCTGCTCAACTTTG
  ATGAGTTTGCTGTCAGCAAGGATGGCATTGAGAAGCTACTGACCATGATGCCCACGGAGGAAGAGCGGCA
  GAAGATTGAGGAAGCCCAGCTGGCCAACCCTGACATACCCCTGGGCCCAGCCGAGAACTTCCTGATGACT
  CTTGCCTCCATTGGCGGCCTCGCTGCTCGTCTACAACTCTGGGCCTTCAAGCTGGACTATGACAGCATGG
  AGCGGGAAATTGCTGAGCCACTGTTTGACCTGAAAGTGGGTATGGAACAGCTGGTACAGAATGCCACCTT
  CCGCTGCATCCTGGCTACCCTCCTAGCGGTGGGCAACTTCCTCAATGGCTCCCAGAGCAGCGGCTTTGAG
  CTGAGCTACCTGGAGAAGGTGTCAGAGGTGAAGGACACGGTGCGTCGACAGTCACTGCTACACCATCTCT
  GCTCCCTAGTGCTCCAGACCCGGCCTGAGTCCTCTGACCTCTATTCAGAAATCCCTGCCCTGACCCGCTG
  TGCCAAGGTGGACTTTGAACAGCTGACTGAGAACCTGGGGCAGCTGGAGCGCCGGAGCCGGGCAGCCGAG
  GAGAGCCTGCGGAGCTTGGCCAAGCATGAGCTGGCCCCAGCCCTGCGTGCCCGCCTCACCCACTTCCTGG
  ACCAGTGTGCCCGCCGTGTTGCCATGCTAAGGATAGTGCACCGCCGTGTCTGCAATAGGTTCCATGCCTT
  CCTGCTCTACCTGGGCTACACCCCGCAGGCGGCCCGTGAAGTGCGCATCATGCAGTTCTGCCACACGCTG
  CGGGAATTTGCGCTTGAGTATCGGACTTGCCGGGAACGAGTGCTACAGCAGCAGCAGAAGCAGGCCACAT
  ACCGTGAGCGCAACAAGACCCGGGGACGCATGATCACCGAGACAGAGAAGTTCTCAGGTGTGGCTGGGGA
  AGCCCCCAGCAACCCCTCTGTCCCAGTAGCAGTGAGCAGCGGGCCAGGCCGGGGAGATGCTGACAGTCAT
  GCTAGTATGAAGAGTCTGCTGACCAGCAGGCCTGAGGACACCACACACAATCGCCGCAGCAGAGGCATGG
  TCCAGAGCAGCTCCCCAATCATGCCCACAGTGGGGCCCTCCACTGCATCCCCAGAAGAACCCCCAGGCTC
  CAGTTTACCCAGTGATACATCAGATGAGATCATGGACCTTCTGGTGCAGTCAGTGACCAAGAGCAGTCCT
  CGTGCCTTAGCTGCTAGGGAACGCAAGCGTTCCCGCGGCAACCGCAAGTCTTTGAGAAGGACGTTGAAGA
  GTGGGCTCGGAGATGACCTGGTGCAGGCACTGGGACTAAGCAAGGGTCCTGGCCTGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207683 representing NM_013241
  Red=Cloning site Green=Tags(s)
  
  MAGGEDRGDGEPVSVVTVRVQYLEDTDPFACANFPEPRRAPTCSLDGALPLGAQIPAVHRLLGAPLKLED
  CALQVSPSGYYLDTELSLEEQREMLEGFYEEISKGRKPTLILRTQLSVRVNAILEKLYSSSGPELRRSLF
  SLKQIFQEDKDLVPEFVHSEGLSCLIRVGAAADHNYQSYILRALGQLMLFVDGMLGVVAHSDTIQWLYTL
  CASLSRLVVKTALKLLLVFVEYSENNAPLFIRAVNSVASTTGAPPWANLVSILEEKNGADPELLVYTVTL
  INKTLAALPDQDSFYDVTDALEQQGMEALVQRHLGTAGTDVDLRTQLVLYENALKLEDGDIEEAPGAGGR
  RERRKPSSEEGKRSRRSLEGGGCPARAPEPGPTGPASPVGPTSSTGPALLTGPASSPVGPPSGLQASVNL
  FPTISVAPSADTSSERSIYKARFLENVAAAETEKQVALAQGRAETLAGAMPNEAGGHPDARQLWDSPETA
  PAARTPQSPAPCVLLRAQRSLAPEPKEPLIPASPKAEPIWELPTRAPRLSIGDLDFSDLGEDEDQDMLNV
  ESVEAGKDIPAPSPPLPLLSGVPPPPPLPPPPPIKGPFPPPPPLPLAAPLPHSVPDSSALPTKRKTVKLF
  WRELKLAGGHGVSASRFGPCATLWASLDPVSVDTARLEHLFESRAKEVLPSKKAGEGRRTMTTVLDPKRS
  NAINIGLTTLPPVHVIKAALLNFDEFAVSKDGIEKLLTMMPTEEERQKIEEAQLANPDIPLGPAENFLMT
  LASIGGLAARLQLWAFKLDYDSMEREIAEPLFDLKVGMEQLVQNATFRCILATLLAVGNFLNGSQSSGFE
  LSYLEKVSEVKDTVRRQSLLHHLCSLVLQTRPESSDLYSEIPALTRCAKVDFEQLTENLGQLERRSRAAE
  ESLRSLAKHELAPALRARLTHFLDQCARRVAMLRIVHRRVCNRFHAFLLYLGYTPQAAREVRIMQFCHTL
  REFALEYRTCRERVLQQQQKQATYRERNKTRGRMITETEKFSGVAGEAPSNPSVPVAVSSGPGRGDADSH
  ASMKSLLTSRPEDTTHNRRSRGMVQSSSPIMPTVGPSTASPEEPPGSSLPSDTSDEIMDLLVQSVTKSSP
  RALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKGPGLEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013241
• ORF Size: 3492 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013241.3
• RefSeq Size: 3865 bp
• RefSeq ORF: 3495 bp
• Locus ID: 29109
• UniProt ID: Q9Y613
• Cytogenetics: 16q22.1
• Domains: FH2
• MW: 126.4 kDa
• Gene Summary: This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]