MTFMT (NM_139242) Human Tagged ORF Clone

CAT#: RC207666

MTFMT (Myc-DDK-tagged)-Human mitochondrial methionyl-tRNA formyltransferase (MTFMT), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC207666

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MTFMT (Cat# RC207666, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTFMT(Cat# TA503565). Positive lysates LY408317 (100ug) and LC408317 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY408317) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207666 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTFMT
• Synonyms: COXPD15; FMT1; MC1DN27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207666 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCCCATCACCAAAAGGACTGCCAGTGAAGCAATATGCTGTGCAGTCTCAGCTTCCCGTATATG
  AGTGGCCGGATGTGGGATCTGGAGAATATGATGTTGGAGTAGTGGCTTCGTTTGGCCGACTTTTGAATGA
  GGCTCTTATTCTTAAATTTCCCTATGGCATATTGAATGTTCATCCCAGTTGCCTCCCGAGATGGCGTGGC
  CCAGCCCCTGTAATCCATACAGTGCTTCACGGAGACACAGTTACTGGAGTAACAATTATGCAAATTAGAC
  CTAAAAGGTTTGATGTAGGCCCAATTCTCAAACAAGAAACTGTTCCTGTGCCACCCAAGAGCACTGCAAA
  GGAATTGGAAGCAGTGTTGTCAAGACTGGGTGCCAACATGCTCATTTCAGTTTTGAAAAATTTGCCTGAA
  AGTCTGAGCAATGGAAGGCAGCAGCCAATGGAGGGGGCGACTTACGCCCCTAAGATTTCTGCTGGTACCA
  GTTGTATAAAATGGGAGGAACAAACTTCAGAACAAATATTCAGACTTTACCGTGCCATTGGAAATATAAT
  TCCGTTGCAGACGCTCTGGATGGCGAATACCATTAAACTTCTGGATTTGGTAGAAGTTAACAGTTCAGTC
  CTTGCTGATCCAAAATTAACGGGACAGGCTCTTATTCCAGGATCAGTAATATACCACAAACAGTCACAAA
  TACTATTGGTTTATTGCAAGGATGGTTGGATTGGTGTTCGATCAGTGATGCTCAAGAAATCACTAACAGC
  TACTGACTTCTACAATGGATATTTGCACCCCTGGTACCAGAAAAATTCCCAAGCTCAACCAAGCCAATGC
  AGATTTCAGACTCTCAGACTTCCAACAAAGAAGAAGCAGAAAAAAACTGTTGCTATGCAACAATGCATTG
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207666 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSPSPKGLPVKQYAVQSQLPVYEWPDVGSGEYDVGVVASFGRLLNEALILKFPYGILNVHPSCLPRWRG
  PAPVIHTVLHGDTVTGVTIMQIRPKRFDVGPILKQETVPVPPKSTAKELEAVLSRLGANMLISVLKNLPE
  SLSNGRQQPMEGATYAPKISAGTSCIKWEEQTSEQIFRLYRAIGNIIPLQTLWMANTIKLLDLVEVNSSV
  LADPKLTGQALIPGSVIYHKQSQILLVYCKDGWIGVRSVMLKKSLTATDFYNGYLHPWYQKNSQAQPSQC
  RFQTLRLPTKKKQKKTVAMQQCIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139242
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139242.2, NP_640335.1
• RefSeq Size: 2763 bp
• RefSeq ORF: 1170 bp
• Locus ID: 123263
• UniProt ID: Q96DP5
• Cytogenetics: 15q22.31
• Domains: formyl_transf
• Protein Pathways: Aminoacyl-tRNA biosynthesis, One carbon pool by folate
• MW: 33.8 kDa
• Gene Summary: The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq, Jun 2011]