Somatostatin Receptor 1 (SSTR1) (NM_001049) Human Tagged ORF Clone

CAT#: RC207589

SSTR1 (Myc-DDK-tagged)-Human somatostatin receptor 1 (SSTR1)

Product Images

Circular map for RC207589

• 

  Western blot validation of overexpression lysate (Cat# LY420798) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207589 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Somatostatin Receptor 1
• Synonyms: SRIF-2; SS-1-R; SS1-R; SS1R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207589 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCCAATGGCACCGCCTCCTCTCCTTCCTCCTCTCCTAGCCCCAGCCCGGGCAGCTGCGGCGAAG
  GCGGCGGCAGCAGGGGCCCCGGGGCCGGCGCTGCGGACGGCATGGAGGAGCCAGGGCGAAATGCGTCCCA
  GAACGGGACCTTGAGCGAGGGCCAGGGCAGCGCCATCCTGATCTCTTTCATCTACTCCGTGGTGTGCCTG
  GTGGGGCTGTGTGGGAACTCTATGGTCATCTACGTGATCCTGCGCTATGCCAAGATGAAGACGGCCACCA
  ACATCTACATCCTAAATCTGGCCATTGCTGATGAGCTGCTCATGCTCAGCGTGCCCTTCCTAGTCACCTC
  CACGTTGTTGCGCCACTGGCCCTTCGGTGCGCTGCTCTGCCGCCTCGTGCTCAGCGTGGACGCGGTCAAC
  ATGTTCACCAGCATCTACTGTCTGACTGTGCTCAGCGTGGACCGCTACGTGGCCGTGGTGCATCCCATCA
  AGGCGGCCCGCTACCGCCGGCCCACCGTGGCCAAGGTAGTAAACCTGGGCGTGTGGGTGCTATCGCTGCT
  CGTCATCCTGCCCATCGTGGTCTTCTCTCGCACCGCGGCCAACAGCGACGGCACGGTGGCTTGCAACATG
  CTCATGCCAGAGCCCGCTCAACGCTGGCTGGTGGGCTTCGTGTTGTACACATTTCTCATGGGCTTCCTGC
  TGCCCGTGGGGGCTATCTGCCTGTGCTACGTGCTCATCATTGCTAAGATGCGCATGGTGGCCCTCAAGGC
  CGGCTGGCAGCAGCGCAAGCGCTCGGAGCGCAAGATCACCTTAATGGTGATGATGGTGGTGATGGTGTTT
  GTCATCTGCTGGATGCCTTTCTACGTGGTGCAGCTGGTCAACGTGTTTGCTGAGCAGGACGACGCCACGG
  TGAGTCAGCTGTCGGTCATCCTCGGCTATGCCAACAGCTGCGCCAACCCCATCCTCTATGGCTTTCTCTC
  AGACAACTTCAAGCGCTCTTTCCAACGCATCCTATGCCTCAGCTGGATGGACAACGCCGCGGAGGAGCCG
  GTTGACTATTACGCCACCGCGCTCAAGAGCCGTGCCTACAGTGTGGAAGACTTCCAACCTGAGAACCTGG
  AGTCCGGCGGCGTCTTCCGTAATGGCACCTGCACGTCCCGGATCACGACGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207589 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPNGTASSPSSSPSPSPGSCGEGGGSRGPGAGAADGMEEPGRNASQNGTLSEGQGSAILISFIYSVVCL
  VGLCGNSMVIYVILRYAKMKTATNIYILNLAIADELLMLSVPFLVTSTLLRHWPFGALLCRLVLSVDAVN
  MFTSIYCLTVLSVDRYVAVVHPIKAARYRRPTVAKVVNLGVWVLSLLVILPIVVFSRTAANSDGTVACNM
  LMPEPAQRWLVGFVLYTFLMGFLLPVGAICLCYVLIIAKMRMVALKAGWQQRKRSERKITLMVMMVVMVF
  VICWMPFYVVQLVNVFAEQDDATVSQLSVILGYANSCANPILYGFLSDNFKRSFQRILCLSWMDNAAEEP
  VDYYATALKSRAYSVEDFQPENLESGGVFRNGTCTSRITTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001049
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001049.3
• RefSeq Size: 4343 bp
• RefSeq ORF: 1176 bp
• Locus ID: 6751
• UniProt ID: P30872
• Cytogenetics: 14q21.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 42.7 kDa
• Gene Summary: Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. The protein encoded by this gene is a member of the superfamily of somatostatin receptors having seven transmembrane segments. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This somatostatin receptor has greater affinity for somatostatin-14 than -28. [provided by RefSeq, Jul 2012]