RGL2 (NM_004761) Human Tagged ORF Clone

CAT#: RC207582

RGL2 (Myc-DDK-tagged)-Human ral guanine nucleotide dissociation stimulator-like 2 (RGL2), transcript variant 1

Product Images

Circular map for RC207582

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RGL2 (Cat# RC207582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGL2 (Cat# TA808391)(1:2000). Positive lysates LY417774 (100ug) and LC417774 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY417774) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207582 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RGL2 protein (Cat# TP307582). The protein was produced from HEK293T cells transfected with RGL2 cDNA clone (Cat# RC207582) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGL2
• Synonyms: HKE1.5; KE1.5; RAB2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207582 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCGCGGCCCCTGCGGCTGCTTTTGGACACGAGCCCCCCCGGGGGAGTCGTACTGAGCAGCTTCC
  GAAGCCGGGACCCCGAAGAGGGTGGGGGCCCAGGTGGCCTGGTCGTGGGCGGGGGGCAGGAGGAAGAGGA
  GGAGGAAGAAGAAGAGGCCCCTGTGTCCGTCTGGGATGAGGAGGAGGATGGTGCCGTGTTTACCGTCACA
  AGCCGCCAATATCGACCTCTTGATCCCTTGGTCCCTATGCCTCCCCCACGTTCCTCCCGACGGCTCCGAG
  CTGGCACTCTGGAGGCCCTGGTCAGACACCTACTGGATACCCGGACATCAGGGACTGATGTGAGCTTCAT
  GTCAGCCTTCCTGGCTACCCACCGGGCCTTCACCTCCACGCCTGCCTTGCTAGGGCTTATGGCTGACAGG
  CTGGAAGCCCTTGAATCTCATCCTACCGACGAACTAGAGAGGACAACAGAGGTAGCCATCTCTGTACTGT
  CAACCTGGCTGGCCTCTCACCCTGAGGATTTTGGCTCTGAGGCCAAGGGTCAGCTTGACCGGCTTGAGAG
  CTTCTTACTTCAGACAGGGTATGCAGCAGGGAAGGGTGTTGGGGGGGGCAGCGCTGACCTCATCCGCAAT
  CTCCGGTCCCGGGTGGACCCCCAGGCCCCCGACCTTCCTAAGCCCCTGGCCCTCCCCGGCGATCCCCCTG
  CTGACCCCACGGATGTCCTGGTGTTCCTCGCTGACCACTTGGCCGAACAGCTGACCCTGCTAGATGCGGA
  ACTTTTTCTCAATTTGATCCCCTCTCAGTGCCTGGGAGGCCTGTGGGGTCACAGAGACCGGCCAGGACAT
  TCTCACCTCTGCCCATCTGTCCGAGCTACTGTCACACAGTTTAACAAGGTGGCAGGGGCAGTGGTTAGTT
  CTGTCCTGGGGGCTACTTCCACTGGAGAGGGACCTGGGGAGGTGACCATACGGCCACTCCGTCCCCCACA
  GAGGGCCCGGCTCCTGGAGAAGTGGATCCGCGTGGCAGAGGAGTGCCGGCTGCTCCGAAACTTCTCTTCA
  GTTTATGCCGTGGTGTCAGCCCTGCAGTCCAGCCCCATCCACAGGCTTCGGGCAGCCTGGGGGGAAGCAA
  CCAGGGACAGCCTCAGAGTCTTTTCCAGCCTCTGCCAGATTTTCTCCGAGGAGGATAATTATTCCCAGAG
  TCGGGAGCTGCTCGTGCAGGAGGTGAAGCTGCAGTCTCCTCTGGAGCCACACTCCAAGAAGGCCCCGAGG
  TCTGGCTCCCGGGGTGGGGGTGTGGTCCCATACCTTGGCACCTTCCTGAAGGACCTTGTGATGCTGGATG
  CAGCCTCCAAGGATGAGTTGGAGAATGGATACATCAATTTTGACAAGCGGAGGAAGGAGTTTGCAGTCCT
  TTCTGAGTTGCGACGGCTCCAGAATGAATGTCGTGGCTATAACCTCCAACCTGACCATGATATCCAGAGG
  TGGCTACAGGGGCTCCGGCCACTGACAGAGGCTCAGAGCCATCGTGTATCCTGTGAGGTGGAGCCACCTG
  GTTCCAGTGACCCTCCTGCCCCACGGGTGCTTCGGCCAACATTGGTCATCTCGCAGTGGACAGAGGTTTT
  GGGCTCTGTTGGGGTCCCTACCCCGCTTGTGTCCTGTGACCGGCCCAGTACTGGGGGAGATGAGGCGCCT
  ACAACTCCTGCTCCTCTGCTGACTCGGCTGGCCCAGCACATGAAGTGGCCATCTGTCTCGTCACTAGACT
  CTGCCTTGGAAAGCAGTCCATCCCTGCACAGTCCAGCTGACCCCAGCCACCTCTCCCCACCAGCCTCCTC
  CCCTAGGCCTTCTCGAGGTCACCGCCGCTCAGCCTCCTGTGGCTCCCCGCTGAGTGGGGGTGCAGAAGAG
  GCCTCCGGGGGGACTGGATATGGGGGAGAGGGATCTGGGCCAGGGGCCTCTGATTGCCGTATCATCCGAG
  TCCAGATGGAGTTGGGGGAAGATGGCAGTGTCTATAAGAGCATTTTGGTGACAAGCCAGGACAAGGCTCC
  AAGTGTCATCAGTCGTGTCCTTAAGAAAAACAATCGTGACTCTGCAGTGGCTTCAGAGTATGAGCTGGTA
  CAGCTGCTACCAGGGGAGCGAGAGCTGACTATCCCAGCCTCGGCTAATGTATTCTACGCCATGGATGGAG
  CTTCACACGATTTCCTCCTGCGGCAGCGGCGAAGGTCCTCTACTGCTACACCTGGCGTCACCAGTGGCCC
  GTCTGCCTCAGGAACTCCTCCGAGTGAGGGAGGAGGGGGCTCCTTTCCCAGGATCAAGGCCACAGGGAGG
  AAGATTGCACGGGCACTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207582 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPRPLRLLLDTSPPGGVVLSSFRSRDPEEGGGPGGLVVGGGQEEEEEEEEEAPVSVWDEEEDGAVFTVT
  SRQYRPLDPLVPMPPPRSSRRLRAGTLEALVRHLLDTRTSGTDVSFMSAFLATHRAFTSTPALLGLMADR
  LEALESHPTDELERTTEVAISVLSTWLASHPEDFGSEAKGQLDRLESFLLQTGYAAGKGVGGGSADLIRN
  LRSRVDPQAPDLPKPLALPGDPPADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGH
  SHLCPSVRATVTQFNKVAGAVVSSVLGATSTGEGPGEVTIRPLRPPQRARLLEKWIRVAEECRLLRNFSS
  VYAVVSALQSSPIHRLRAAWGEATRDSLRVFSSLCQIFSEEDNYSQSRELLVQEVKLQSPLEPHSKKAPR
  SGSRGGGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAVLSELRRLQNECRGYNLQPDHDIQR
  WLQGLRPLTEAQSHRVSCEVEPPGSSDPPAPRVLRPTLVISQWTEVLGSVGVPTPLVSCDRPSTGGDEAP
  TTPAPLLTRLAQHMKWPSVSSLDSALESSPSLHSPADPSHLSPPASSPRPSRGHRRSASCGSPLSGGAEE
  ASGGTGYGGEGSGPGASDCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEYELV
  QLLPGERELTIPASANVFYAMDGASHDFLLRQRRRSSTATPGVTSGPSASGTPPSEGGGGSFPRIKATGR
  KIARALF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004761
• ORF Size: 2331 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004761.5
• RefSeq Size: 3362 bp
• RefSeq ORF: 2334 bp
• Locus ID: 5863
• UniProt ID: O15211
• Cytogenetics: 6p21.32
• Domains: RA, RasGEFN, RasGEF
• Protein Families: Druggable Genome
• MW: 83.5 kDa
• Gene Summary: Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro (By similarity).[UniProtKB/Swiss-Prot Function]