IFT140 (NM_014714) Human Tagged ORF Clone

CAT#: RC207528

IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140)

Product Images

Circular map for RC207528

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFT140
• Synonyms: c305C8.4; c380F5.1; gs114; MZSDS; RP80; SRTD9; WDTC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCTATTATGACCACCAGATAGAAGCCCCGGATGCAGCAGGGTCACCCTCATTTATCAGCTGGC
  ACCCTGTCCATCCATTCTTGGCAGTTGCTTACATCAGCACAACCTCAACAGGCAGCGTGGATATTTACCT
  GGAGCAAGGGGAGTGCGTGCCAGATACACACGTCGAGAGGCCGTTCCGGGTTGCTTCCCTGTGCTGGCAC
  CCGACGCGGCTGGTGCTGGCTGTGGGCTGGGAGACTGGAGAAGTGACGGTGTTTAACAAGCAGGACAAGG
  AGCAGCACACGATGCCCCTGACACACACAGCCGACATCACCGTGCTCCGTTGGAGCCCCAGTGGAAACTG
  CCTGCTGTCTGGGGACAGGCTTGGTGTCTTGCTCTTGTGGAGGTTGGACCAAAGGGGCCGAGTGCAAGGG
  ACGCCTCTGCTGAAACACGAGTATGGGAAACACCTCACGCACTGCATCTTCCGGCTCCCCCCTCCTGGCG
  AGGACCTGGTTCAGTTGGCAAAGGCAGCTGTGAGCGGTGATGAGAAAGCCCTGGACATGTTTAACTGGAA
  GAAGAGCAGTTCTGGAAGTTTGCTGAAGATGGGGTCTCACGAGGGGCTGTTGTTCTTTGTCAGTCTGATG
  GACGGGACAGTGCACTATGTGGATGAGAAGGGCAAGACCACTCAGGTGGTGTCCGCAGACAGCACGATTC
  AGATGCTGTTCTACATGGAGAAGAGGGAGGCACTGGTGGTGGTCACAGAGAACCTCCGGCTGTCCCTGTA
  CACGGTGCCTCCTGAGGGCAAAGCAGAAGAAGTGATGAAGGTCAAGCTGAGCGGGAAAACCGGCCGCCGG
  GCAGACATCGCTTTGATTGAAGGCAGCCTTCTCGTGATGGCCGTCGGGGAGGCTGCCCTCAGATTCTGGG
  ACATAGAACGAGGAGAGAATTATATACTGAGTCCAGATGAGAAGTTTGGCTTTGAGAAAGGAGAGAATAT
  GAACTGTGTGTGTTACTGTAAAGTCAAAGGTCTTCTGGCCGCTGGTACCGACAGAGGGCGAGTAGCCATG
  TGGAGGAAAGTACCAGACTTCCTGGGCAGCCCCGGGGCAGAGGGCAAGGACAGGTGGGCCCTTCAGACCC
  CTACCGAGCTCCAAGGAAACATCACGCAAATCCAGTGGGGTTCCAGGAAGAACCTGCTGGCAGTGAACAG
  CGTCATCTCCGTGGCCATCCTCAGCGAGCGGGCCATGTCGTCACACTTCCACCAGCAAGTGGCCGCCATG
  CAGGTCTCCCCGAGTCTGCTGAATGTGTGCTTCCTGTCCACGGGGGTCGCACACAGCCTGCGCACCGACA
  TGCACATCAGTGGAGTGTTTGCCACCAAGGATGCTGTCGCAGTCTGGAACGGAAGGCAGGTGGCGATCTT
  CGAGCTTTCTGGAGCCGCGATACGGAGTGCAGGGACCTTCTTGTGTGAGACGCCTGTGTTAGCAATGCAT
  GAAGAAAACGTTTACACGGTGGAGTCAAACCGAGTTCAAGTTCGAACCTGGCAGGGGACTGTCAAACAAC
  TCCTCCTTTTCTCGGAGACTGAGGGGAATCCCTGCTTCTTGGACATCTGTGGGAATTTCCTGGTTGTAGG
  GACAGACTTGGCTCACTTTAAAAGCTTTGATCTTTCCCGAAGAGAGGCCAAAGCACACTGTAGCTGCAGG
  AGCCTGGCGGAGCTGGTCCCTGGGGTGGGGGGCATCGCTTCTCTGCGGTGCAGCAGCAGCGGGAGCACCA
  TCAGCATCCTCCCCAGCAAGGCTGACAACAGCCCTGATTCCAAAATCTGCTTCTACGATGTTGAAATGGA
  CACAGTGACCGTCTTTGACTTCAAGACTGGACAAATTGATCGGAGAGAGACGCTGTCCTTTAATGAGCAA
  GAGACTAATAAGAGCCACCTCTTTGTGGATGAGGGACTGAAAAATTATGTTCCCGTGAACCACTTCTGGG
  ACCAGAGTGAGCCCCGGCTGTTTGTATGCGAAGCCGTGCAGGAGACGCCGCGCTCCCAGCCTCAGTCTGC
  AAACGGGCAGCCCCAAGATGGGCGCGCTGGCCCTGCGGCAGATGTTTTGATCCTGTCCTTCTTCATTTCC
  GAAGAGCACGGCTTCCTGCTTCATGAGAGCTTCCCCCGGCCTGCCACCTCCCACAGTCTCCTGGGGATGG
  AAGTGCCTTATTACTACTTCACAAGAAAGCCCGAAGAAGCAGACAGAGAAGACGAGGTGGAGCCTGGGTG
  CCACCACATCCCTCAGATGGTGTCCAGGAGACCCCTGCGAGACTTTGTGGGGCTGGAGGACTGCGACAAG
  GCCACCCGGGACGCCATGCTCCACTTCAGCTTCTTTGTCACCATAGGAGACATGGACGAAGCCTTCAAAT
  CCATCAAGCTCATCAAAAGTGAGGCCGTCTGGGAGAACATGGCGCGCATGTGCGTGAAGACCCAGCGGCT
  GGACGTGGCCAAGGTGTGCCTGGGGAACATGGGCCATGCCCGCGGGGCCCGAGCGCTGCGTGAGGCGGAG
  CAGGAGCCGGAGCTAGAGGCCCGCGTGGCCGTGCTGGCCACGCAGCTGGGCATGCTGGAGGACGCCGAGC
  AGCTGTACAGGAAGTGCAAGCGCCACGACCTCCTGAACAAGTTCTACCAGGCTGCGGGCCGGTGGCAGGA
  GGCCCTCCAGGTAGCCGAGCACCACGATCGCGTGCACCTGCGCAGCACCTACCACCGCTATGCCGGGCAC
  CTGGAGGCCAGCGCCGACTGCAGCCGGGCCCTCAGTTACTACGAGAAGTCGGACACGCACCGCTTCGAGG
  TGCCCAGGATGCTGTCGGAGGACCTGCCGTCCCTGGAGCTCTACGTGAATAAAATGAAGGATAAGACCCT
  GTGGCGGTGGTGGGCGCAGTACCTGGAGAGCCAGGGCGAGATGGACGCCGCGCTGCACTACTACGAGCTG
  GCCCGGGACCACTTCTCCCTGGTCCGCATCCACTGCTTCCAGGGCAATGTCCAGAAGGCTGCGCAAATAG
  CCAACGAGACAGGAAACCTGGCGGCCTCCTACCACCTCGCCCGCCAGTACGAGAGCCAGGAGGAGGTCGG
  GCAGGCGGTGCACTTCTACACCCGGGCACAGGCCTTCAAGAATGCCATCCGCCTGTGCAAGGAGAACGGC
  CTGGACGACCAGCTCATGAACTTGGCCCTGCTGAGCTCCCCCGAGGACATGATCGAGGCGGCCCGATACT
  ACGAGGAGAAGGGCGTGCAGATGGACAGGGCGGTCATGCTGTACCACAAGGCTGGCCACTTCTCCAAGGC
  CCTGGAGCTGGCCTTTGCCACCCAGCAGTTTGTGGCCCTACAGCTCATAGCAGAGGACCTGGATGAGACG
  TCAGACCCTGCGCTCCTGGCCCGCTGCTCCGACTTCTTCATCGAGCACAGTCAGTACGAGAGGGCGGTAG
  AGCTGCTGCTGGCTGCCAGGAAGTATCAGGAAGCCCTGCAGCTGTGCCTGGGGCAGAACATGAGCATCAC
  CGAGGAGATGGCGGAAAAGATGACCGTGGCCAAGGACTCCTCGGACCTGCCTGAGGAGTCGCGGCGGGAG
  CTGCTGGAGCAGATAGCAGACTGCTGCATGCGCCAGGGCAGCTACCACCTGGCCACCAAGAAGTACACGC
  AGGCCGGCAACAAGCTGAAGGCCATGAGGGCGCTGCTCAAATCCGGAGACACGGAGAAAATCACGTTCTT
  CGCGAGCGTGTCCAGGCAGAAGGAAATCTACATCATGGCTGCTAACTACCTGCAGTCCCTGGACTGGCGG
  AAGGAGCCGGAGATCATGAAGAACATCATCGGCTTCTACACCAAGGGGCGGGCCCTGGACCTCCTGGCTG
  GCTTTTATGACGCTTGTGCCCAGGTGGAGATTGATGAATACCAGAACTACGACAAAGCCCACGGGGCGCT
  GACCGAGGCCTACAAGTGCCTGGCCAAGGCCAAGGCCAAGAGCCCCCTGGACCAGGAGACCAGGCTGGCG
  CAGCTGCAGAGCAGGATGGCACTGGTGAAGAGGTTCATCCAGGCCCGCAGGACGTACACAGAGGACCCCA
  AGGAGTCCATCAAGCAGTGTGAGCTGCTCCTGGAGGAACCAGACCTGGACAGCACCATCCGCATCGGGGA
  CGTCTATGGCTTCCTGGTGGAGCACTACGTGCGGAAGGAGGAATACCAGACGGCCTACAGATTCCTGGAG
  GAGATGCGGCGGCGGCTTCCCTTGGCCAACATGTCCTACTACGTGAGCCCGCAGGCCGTGGACGCCGTGC
  ACCGGGGGCTGGGTCTCCCACTGCCACGCACCGTCCCCGAGCAGGTCCGCCACAACAGCATGGAGGACGC
  CAGGGAGCTGGACGAGGAGGTGGTGGAAGAGGCAGATGACGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALYYDHQIEAPDAAGSPSFISWHPVHPFLAVAYISTTSTGSVDIYLEQGECVPDTHVERPFRVASLCWH
  PTRLVLAVGWETGEVTVFNKQDKEQHTMPLTHTADITVLRWSPSGNCLLSGDRLGVLLLWRLDQRGRVQG
  TPLLKHEYGKHLTHCIFRLPPPGEDLVQLAKAAVSGDEKALDMFNWKKSSSGSLLKMGSHEGLLFFVSLM
  DGTVHYVDEKGKTTQVVSADSTIQMLFYMEKREALVVVTENLRLSLYTVPPEGKAEEVMKVKLSGKTGRR
  ADIALIEGSLLVMAVGEAALRFWDIERGENYILSPDEKFGFEKGENMNCVCYCKVKGLLAAGTDRGRVAM
  WRKVPDFLGSPGAEGKDRWALQTPTELQGNITQIQWGSRKNLLAVNSVISVAILSERAMSSHFHQQVAAM
  QVSPSLLNVCFLSTGVAHSLRTDMHISGVFATKDAVAVWNGRQVAIFELSGAAIRSAGTFLCETPVLAMH
  EENVYTVESNRVQVRTWQGTVKQLLLFSETEGNPCFLDICGNFLVVGTDLAHFKSFDLSRREAKAHCSCR
  SLAELVPGVGGIASLRCSSSGSTISILPSKADNSPDSKICFYDVEMDTVTVFDFKTGQIDRRETLSFNEQ
  ETNKSHLFVDEGLKNYVPVNHFWDQSEPRLFVCEAVQETPRSQPQSANGQPQDGRAGPAADVLILSFFIS
  EEHGFLLHESFPRPATSHSLLGMEVPYYYFTRKPEEADREDEVEPGCHHIPQMVSRRPLRDFVGLEDCDK
  ATRDAMLHFSFFVTIGDMDEAFKSIKLIKSEAVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAE
  QEPELEARVAVLATQLGMLEDAEQLYRKCKRHDLLNKFYQAAGRWQEALQVAEHHDRVHLRSTYHRYAGH
  LEASADCSRALSYYEKSDTHRFEVPRMLSEDLPSLELYVNKMKDKTLWRWWAQYLESQGEMDAALHYYEL
  ARDHFSLVRIHCFQGNVQKAAQIANETGNLAASYHLARQYESQEEVGQAVHFYTRAQAFKNAIRLCKENG
  LDDQLMNLALLSSPEDMIEAARYYEEKGVQMDRAVMLYHKAGHFSKALELAFATQQFVALQLIAEDLDET
  SDPALLARCSDFFIEHSQYERAVELLLAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRE
  LLEQIADCCMRQGSYHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWR
  KEPEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLA
  QLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKEEYQTAYRFLE
  EMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVRHNSMEDARELDEEVVEEADDDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014714
• ORF Size: 4386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014714.4
• RefSeq Size: 5277 bp
• RefSeq ORF: 4389 bp
• Locus ID: 9742
• UniProt ID: Q96RY7
• Cytogenetics: 16p13.3
• Domains: WD40
• MW: 165.2 kDa
• Gene Summary: This gene encodes one of the subunits of the intraflagellar transport (IFT) complex A. Intraflagellar transport is involved in the genesis, resorption and signaling of primary cilia. The primary cilium is a microtubule-based sensory organelle at the surface of most quiescent mammalian cells, that receives signals from its environment, such as the flow of fluid, light or odors, and transduces those signals to the nucleus. Loss of the corresponding protein in mouse results in renal cystic disease. [provided by RefSeq, Jun 2012]