IPO11 (NM_016338) Human Tagged ORF Clone

CAT#: RC207519

IPO11 (Myc-DDK-tagged)-Human importin 11 (IPO11), transcript variant 2

Product Images

Circular map for RC207519

• 

  Western blot validation of overexpression lysate (Cat# LY402541) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207519 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified IPO11 protein (Cat# TP307519). The protein was produced from HEK293T cells transfected with IPO11 cDNA clone (Cat# RC207519) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IPO11
• Synonyms: RanBP11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207519 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCAATAGTGCCAGCACTGTTGTTCTTCAGGTGTTAACACAGGCCACCAGTCAGGATACTGCTG
  TGTTAAAACCAGCTGAGGAGCAGTTGAAGCAGTGGGAGACACAGCCAGGTTTCTATTCAGTGTTGCTGAA
  TATTTTCACCAACCACACTTTGGATATAAATGTAAGGTGGCTTGCTGTACTGTATTTTAAACATGGAATT
  GATCGCTACTGGAGACGTGTAGCACCTCATGCTCTCTCAGAGGAGGAGAAAACTACTCTGCGTGCAGGGC
  TCATCACCAACTTCAATGAACCAATAAACCAGATTGCAACTCAGATTGCAGTGCTCATTGCAAAAGTTGC
  TAGATTGGATTGTCCCAGACAGTGGCCTGAACTAATTCCCACTCTTATAGAGTCTGTTAAAGTCCAGGAT
  GATCTTCGACAGCACAGAGCATTACTTACCTTCTATCATGTTACCAAGACACTGGCATCTAAACGACTTG
  CTGCTGATAGAAAACTATTTTATGATTTAGCTTCTGGAATTTATAATTTTGCCTGCTCTCTGTGGAATCA
  CCACACAGACACATTCCTGCAAGAAGTTTCTTCTGGCAATGAAGCTGCAATTTTGAGTTCACTAGAACGA
  ACACTGCTATCATTGAAAGTGCTGCGTAAGTTAACTGTTAATGGATTTGTGGAACCTCATAAGAATATGG
  AGGTGATGGGTTTTTTACATGGAATATTTGAACGTCTAAAACAGTTTCTGGAATGCAGTAGAAGTATAGG
  TACAGATAATGTTTGTAGAGATAGACTGGAAAAGACCATCATTCTTTTTACTAAAGTGCTTTTGGACTTC
  TTGGATCAGCATCCTTTTTCATTTACTCCTCTAATTCAGAGATCACTGGAATTTTCTGTAAGCTATGTTT
  TTACAGAAGTTGGTGAAGGCGTTACATTTGAACGATTCATTGTCCAATGTATGAATCTTATTAAGATGAT
  TGTCAAAAATTATGCTTATAAGCCATCCAAAAATTTTGAAGATAGCAGCCCTGAAACTCTTGAAGCCCAT
  AAGATTAAGATGGCATTCTTCACATATCCTACTTTGACAGAGATATGTAGAAGATTAGTCTCTCATTATT
  TCCTATTAACTGAAGAAGAACTGACAATGTGGGAAGAAGACCCAGAAGGCTTTACAGTGGAAGAAACAGG
  AGGAGATTCTTGGAAATATAGTTTGAGGCCATGCACTGAAGTATTATTTATAGATATATTCCATGAATAT
  AATCAGACTCTTACTCCTGTACTTCTAGAAATGATGCAAACACTTCAAGGACCCACAAATGTGGAAGATA
  TGAATGCACTGTTAATCAAAGATGCTGTGTATAATGCTGTTGGATTAGCTGCTTATGAGCTCTTTGACAG
  TGTTGATTTTGATCAGTGGTTTAAAAACCAGCTTCTTCCAGAATTACAAGTCATTCACAATAGGTATAAG
  CCATTGCGACGCAGGGTGATTTGGCTCATCGGTCAGTGGATTTCTGTGAAATTCAAGTCTGACTTAAGAC
  CCATGCTTTATGAAGCAATCTGTAACTTGCTTCAAGATCAAGATTTAGTGGTCCGTATTGAAACAGCTAC
  AACTTTGAAGTTAACTGTTGATGATTTTGAATTTAGAACAGATCAGTTTCTACCGTATTTGGAAACCATG
  TTCACACTACTTTTTCAGTTACTGCAGCAAGTTACAGAATGTGACACAAAGATGCATGTTTTGCATGTCC
  TTTCTTGTGTGATCGAAAGAGTCAACATGCAGATACGACCATATGTGGGATGTTTGGTACAATATTTGCC
  CCTCCTTTGGAAGCAGAGTGAAGAACACAATATGTTGAGATGTGCTATTTTGACAACACTTATTCATCTT
  GTTCAGGGATTAGGAGCAGACAGCAAGAACCTGTACCCTTTCCTGCTCCCAGTTATTCAACTGAGTACAG
  ATGTTTCACAGCCTCCACATGTTTATCTTCTGGAAGATGGTTTAGAATTATGGTTAGTAACTTTGGAAAA
  CAGTCCATGTATTACACCAGAGTTGCTTCGTATATTTCAGAATATGTCACCACTTCTTGAACTAAGTTCA
  GAAAATCTTAGAACTTGCTTTAAGATCATCAATGGTTATATCTTTTTATCATCAACAGAATTTTTACAGA
  CATACGCAGTAGGTCTATGCCAGTCCTTTTGTGAACTTTTAAAGGAAATTACTACAGAAGGTCAAGTTCA
  GGTGCTCAAGGTTGTGGAAAATGCCCTTAAAGTGAACCCAATACTAGGTCCACAAATGTTTCAACCGATT
  TTACCCTATGTTTTCAAGGGTATTATAGAAGGGGAGAGGTATCCTGTAGTGATGTCCACGTATCTTGGAG
  TTATGGGTCGAGTTCTACTACAAAACACTAGTTTTTTTTCTTCACTACTTAATGAGATGGCCCATAAATT
  TAATCAGGAGATGGACCAGCTTTTGGGAAATATGATTGAAATGTGGGTTGATCGAATGGACAACATTACC
  CAGCCTGAAAGAAGAAAACTTTCAGCTTTGGCTTTGCTCTCTCTTCTGCCATCTGATAATAGTGTTATCC
  AAGATAAATTCTGTGGGATTATAAACATTTCAGTAGAAGGCCTGCATGATGTCATGACGGAAGATCCTGA
  AACAGGAACTTATAAAGACTGTATGTTGATGTCTCATCTTGAGGAACCAAAAGTAACAGAAGATGAAGAA
  CCACCCACAGAACAAGATAAGAGGAAAAAGATGCTGGCCCTGAAGGACCCTGTTCATACAGTGTCACTGC
  AGCAGTTCATCTACGAGAAGCTCAAGGCACAGCAGGAGATGCTAGGAGAACAAGGTTTCCAGTCCCTCAT
  GGAAACAGTGGATACGGAGATTGTCACCCAGCTACAGGAGTTTTTGCAAGGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207519 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLNSASTVVLQVLTQATSQDTAVLKPAEEQLKQWETQPGFYSVLLNIFTNHTLDINVRWLAVLYFKHGI
  DRYWRRVAPHALSEEEKTTLRAGLITNFNEPINQIATQIAVLIAKVARLDCPRQWPELIPTLIESVKVQD
  DLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYNFACSLWNHHTDTFLQEVSSGNEAAILSSLER
  TLLSLKVLRKLTVNGFVEPHKNMEVMGFLHGIFERLKQFLECSRSIGTDNVCRDRLEKTIILFTKVLLDF
  LDQHPFSFTPLIQRSLEFSVSYVFTEVGEGVTFERFIVQCMNLIKMIVKNYAYKPSKNFEDSSPETLEAH
  KIKMAFFTYPTLTEICRRLVSHYFLLTEEELTMWEEDPEGFTVEETGGDSWKYSLRPCTEVLFIDIFHEY
  NQTLTPVLLEMMQTLQGPTNVEDMNALLIKDAVYNAVGLAAYELFDSVDFDQWFKNQLLPELQVIHNRYK
  PLRRRVIWLIGQWISVKFKSDLRPMLYEAICNLLQDQDLVVRIETATTLKLTVDDFEFRTDQFLPYLETM
  FTLLFQLLQQVTECDTKMHVLHVLSCVIERVNMQIRPYVGCLVQYLPLLWKQSEEHNMLRCAILTTLIHL
  VQGLGADSKNLYPFLLPVIQLSTDVSQPPHVYLLEDGLELWLVTLENSPCITPELLRIFQNMSPLLELSS
  ENLRTCFKIINGYIFLSSTEFLQTYAVGLCQSFCELLKEITTEGQVQVLKVVENALKVNPILGPQMFQPI
  LPYVFKGIIEGERYPVVMSTYLGVMGRVLLQNTSFFSSLLNEMAHKFNQEMDQLLGNMIEMWVDRMDNIT
  QPERRKLSALALLSLLPSDNSVIQDKFCGIINISVEGLHDVMTEDPETGTYKDCMLMSHLEEPKVTEDEE
  PPTEQDKRKKMLALKDPVHTVSLQQFIYEKLKAQQEMLGEQGFQSLMETVDTEIVTQLQEFLQGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016338
• ORF Size: 2925 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016338.5
• RefSeq Size: 4420 bp
• RefSeq ORF: 2928 bp
• Locus ID: 51194
• UniProt ID: Q9UI26
• Cytogenetics: 5q12.1
• Domains: IBN_NT
• MW: 112.5 kDa
• Gene Summary: Importins, including IPO11, are a members of the karyopherin/importin-beta family of transport receptors (see KPNB1; 602738) that mediate nucleocytoplasmic transport of protein and RNA cargoes (Plafker and Macara, 2000 [PubMed 11032817]).[supplied by OMIM, Sep 2008]