Centaurin alpha 2 (ADAP2) (NM_018404) Human Tagged ORF Clone

CAT#: RC207501

ADAP2 (Myc-DDK-tagged)-Human ArfGAP with dual PH domains 2 (ADAP2)

Product Images

Circular map for RC207501

• 

  Western blot validation of overexpression lysate (Cat# LY413076) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207501 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Centaurin alpha 2
• Synonyms: cent-b; CENTA2; HSA272195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207501 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGCGATCGCGAGCGCAACAAGAAGCGGCTGCTGGAGCTGCTGCGGGCGCCGGACACAGGCAACGCGC
  ACTGCGCCGACTGCGGGGCGGCAGATCCCGACTGGGCCTCTTACAAGCTGGGGATCTTCATCTGTCTCAA
  CTGCTGCGGCGTCCACCGTAACTTCCCTGACATCAGCAGAGTTAAATCTGTGCGACTTGACTTCTGGGAC
  GACAGTATTGTGGAGTTTATGATCCACAATGGAAACCTCCGTGTGAAGGCCAAGTTCGAAGCCAGAGTCC
  CAGCTTTCTACTACATCCCCCAGGCCAACGACTGCCTGGTCTTAAAGGAACAATGGATTCGAGCTAAGTA
  TGAGAGACGGGAATTTATGGCTGATGGGGAAACCATCTCGCTCCCAGGTAACCGAGAAGGATTCCTGTGG
  AAGCGAGGAAGGGACAACTCACAGTTTCTGAGAAGGAAGTTTGTACTTCTGGCAAGAGAAGGCCTCCTGA
  AGTACTTCACAAAGGAACAGGGTAAAAGCCCCAAAGCTGTCATCAGCATTAAGGACTTGAATGCCACCTT
  CCAGACAGAGAAGATAGGGCACCCCCATGGGCTGCAGATCACCTACAGGAGAGATGGCCACACCAGGAAC
  CTGTTTGTGTATCATGAAAGTGGGAAGGAGATAGTGGACTGGTTCAATGCCCTCCGTGCAGCCCGTCTGC
  AGTACCTAAAAATGGCCTTTCCTGAACTCCCAGAGTCTGAGCTCGTGCCATTCCTCACCAGGAACTACCT
  CAAACAAGGCTTCATGGAAAAGACTGGGCCAAAGAAAGAACCTTTCAAGAAAAGGTGGTTCGCCCTGGAT
  TGCCATGAGCGGAGGCTGCTCTATTACAAGAACCCACTGGATGCCTTCGAGCAGGGCCAGGTTTTTCTTG
  GGAACAAGGAGCAGGGATATGAAGCCTACGAAGACCTGCCCAAGGGCATCCGAGGAAATCGCTGGAAAGC
  CGGACTCACCATTGTCACCCCAGAGCGGAGATTTGTCCTCACTTGCCCCAGTGAGAAGGAACAGCAGGAA
  TGGCTGGAAAGTTTGCGGGGTGTCCTGTCCAGCCCCTTGACGCCCCTCAACCGGCTTACTGCATCAACAG
  AGAGTGGCCGCAGCAGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDRERNKKRLLELLRAPDTGNAHCADCGAADPDWASYKLGIFICLNCCGVHRNFPDISRVKSVRLDFWD
  DSIVEFMIHNGNLRVKAKFEARVPAFYYIPQANDCLVLKEQWIRAKYERREFMADGETISLPGNREGFLW
  KRGRDNSQFLRRKFVLLAREGLLKYFTKEQGKSPKAVISIKDLNATFQTEKIGHPHGLQITYRRDGHTRN
  LFVYHESGKEIVDWFNALRAARLQYLKMAFPELPESELVPFLTRNYLKQGFMEKTGPKKEPFKKRWFALD
  CHERRLLYYKNPLDAFEQGQVFLGNKEQGYEAYEDLPKGIRGNRWKAGLTIVTPERRFVLTCPSEKEQQE
  WLESLRGVLSSPLTPLNRLTASTESGRSSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018404
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018404.3
• RefSeq Size: 2757 bp
• RefSeq ORF: 1146 bp
• Locus ID: 55803
• UniProt ID: Q9NPF8
• Cytogenetics: 17q11.2
• Domains: ArfGap, PH
• MW: 44.2 kDa
• Gene Summary: The protein encoded by this gene binds beta-tubulin and increases the stability of microtubules. The encoded protein can also translocate to the cell membrane and bind phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). In addition, this protein is a GTPase-activating protein for ADP ribosylation factor 6 and may be able to block the entry of some RNA viruses. [provided by RefSeq, Oct 2016]