CPA6 (NM_020361) Human Tagged ORF Clone

CAT#: RC207491

CPA6 (Myc-DDK-tagged)-Human carboxypeptidase A6 (CPA6)

Product Images

Circular map for RC207491

• 

  Western blot validation of overexpression lysate (Cat# LY412531) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207491 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPA6
• Synonyms: CPAH; ETL5; FEB11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207491 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGTCTCGGGAAGCGCAGGGGCCAGGCAGCTGCTTTCCTGCCTCTTTGCTGGCTCTTTTTGAAGA
  TTCTGCAACCGGGGCACAGCCACCTTTATAACAACCGCTATGCTGGTGATAAAGTGATAAGATTTATTCC
  CAAAACAGAAGAGGAAGCATATGCACTGAAGAAAATATCCTATCAACTTAAGGTGGACCTGTGGCAGCCC
  AGCAGTATCTCCTATGTATCAGAGGGAACAGTTACTGATGTCCATATCCCCCAAAATGGTTCCCGAGCCC
  TGTTAGCCTTCTTACAGGAAGCCAACATCCAGTACAAGGTCCTCATAGAAGATCTTCAGAAAACACTGGA
  GAAGGGAAGCAGCTTGCACACCCAGAGAAACCGAAGATCCCTCTCTGGATATAATTATGAAGTTTATCAC
  TCCTTAGAAGAAATTCAAAATTGGATGCATCATCTGAATAAAACTCACTCAGGCCTCATTCACATGTTCT
  CTATTGGAAGATCATATGAGGGAAGATGTCTTTTTATTTTAAAGCTGGGCAGACGATCACGACTCAAAAG
  AGCTGTTTGGATAGACTGTGGTATTCATGCAAGAGAATGGATTGGTCCTGCCTTTTGTCAGTGGTTTGTA
  AAAGAAGCTCTTCTAACATATAAGAGTGACCCAGCCATGAGAAAAATGTTGAATCATCTATATTTCTATA
  TCATGCCTGTGTTTAACGTCGATGGATACCATTTTAGTTGGACCAATGATCGATTTTGGAGAAAAACAAG
  GTCAAGGAACTCAAGGTTTCGCTGCCGTGGAGTGGATGCCAATAGAAACTGGAAAGTGAAGTGGTGTGAT
  GAAGGAGCTTCTATGCACCCTTGTGATGACACATACTGTGGCCCTTTTCCAGAATCTGAGCCGGAAGTGA
  AGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAGCACATTAGGGCTTATCTCTCCTTTCATGCATATGC
  TCAGATGTTACTGTATCCCTATTCTTACAAATATGCAACAATTCCCAATTTTAGATGTGTGGAATCTGCA
  GCTTATAAAGCTGTGAATGCACTTCAGTCAGTATACGGGGTACGATACAGATATGGACCAGCCTCCACAA
  CGTTGTATGTGAGCTCTGGTAGCTCAATGGATTGGGCCTACAAAAATGGAATACCTTATGCATTTGCTTT
  CGAACTACGTGACACTGGATATTTTGGATTTTTACTCCCAGAGATGCTCATCAAACCCACCTGTACAGAA
  ACTATGCTGGCTGTGAAAAATATCACAATGCACCTGCTAAAGAAATGTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207491 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKCLGKRRGQAAAFLPLCWLFLKILQPGHSHLYNNRYAGDKVIRFIPKTEEEAYALKKISYQLKVDLWQP
  SSISYVSEGTVTDVHIPQNGSRALLAFLQEANIQYKVLIEDLQKTLEKGSSLHTQRNRRSLSGYNYEVYH
  SLEEIQNWMHHLNKTHSGLIHMFSIGRSYEGRCLFILKLGRRSRLKRAVWIDCGIHAREWIGPAFCQWFV
  KEALLTYKSDPAMRKMLNHLYFYIMPVFNVDGYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCD
  EGASMHPCDDTYCGPFPESEPEVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVESA
  AYKAVNALQSVYGVRYRYGPASTTLYVSSGSSMDWAYKNGIPYAFAFELRDTGYFGFLLPEMLIKPTCTE
  TMLAVKNITMHLLKKCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020361
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020361.3
• RefSeq Size: 1907 bp
• RefSeq ORF: 1314 bp
• Locus ID: 57094
• UniProt ID: Q8N4T0
• Cytogenetics: 8q13.2
• Protein Families: Protease, Secreted Protein
• MW: 51 kDa
• Gene Summary: The gene encodes a member of the peptidase M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which catalyzes the release of large hydrophobic C-terminal amino acids. This enzyme has functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Mutations in this gene may be linked to epilepsy and febrile seizures, and a translocation t(6;8)(q26;q13) involving this gene has been associated with Duane retraction syndrome. [provided by RefSeq, May 2016]