TSPAN10 (NM_031945) Human Tagged ORF Clone

CAT#: RC207457

TSPAN10 (Myc-DDK-tagged)-Human tetraspanin 10 (TSPAN10)

Product Images

Circular map for RC207457

• 

  Western blot validation of overexpression lysate (Cat# LY410428) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207457 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TSPAN10 protein (Cat# TP307457). The protein was produced from HEK293T cells transfected with TSPAN10 cDNA clone (Cat# RC207457) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TSPAN10
• Synonyms: OCSP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207457 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGGGGAGAGGAGCCCCTTACTGTCCCAGGAAACTGCAGGCCAGAAGCCCCTCTCTGTGCACA
  GGCCACCCACCTCAGGCTGCCTAGGTCCAGTGCCCAGGGAGGACCAGGCGGAGGCCTGGGGCTGCAGCTG
  CTGTCCCCCGGAGACCAAGCACCAGGCCTTGAGTGGCACTCCCAAGAAAGGACCAGCCCCTTCCCTCTCC
  CCAGGGAGCAGCTGCGTCAAGTATCTGATCTTCCTCTCCAACTTCCCCTTCTCCCTGCTGGGGCTGCTGG
  CCCTGGCCATCGGGCTCTGGGGCCTGGCTGTCAAGGGGTCTCTGGGAAGTGATCTGGGGGGGCCCCTGCC
  CACAGACCCCATGCTGGGGCTGGCACTGGGAGGGCTGGTGGTCAGCGCAGCGAGCCTGGCTGGCTGCCTG
  GGCGCCCTCTGCGAGAACACCTGCCTGTTACGTGGCTTCTCCGGGGGCATCCTTGCCTTCCTGGTGCTTG
  AGGCCGTGGCGGGGGCCCTGGTGGTGGCCCTCTGGGGCCCGCTGCAAGACAGCCTGGAGCACACCCTGCG
  TGTGGCCATCGCCCACTACCAGGACGACCCAGACCTGCGCTTCCTCCTCGACCAAGTCCAGCTCGGGCTG
  AGGTGCTGCGGAGCTGCCTCCTACCAGGACTGGCAGCAGAACCTGTACTTTAACTGCAGCTCCCCCGGGG
  TGCAGGCCTGCAGCCTTCCCGCCTCCTGCTGCATCGACCCCCGCGAAGATGGAGCCTCTGTCAACGACCA
  GTGCGGCTTCGGGGTCCTGCGCCTGGATGCGGACGCAGCTCAGAGAGTGGTGTACCTGGAGGGCTGCGGC
  CCGCCGCTCCGGCGGTGGCTGCGCGCGAACCTGGCTGCCTCGGGCGGCTACGCAATCGCGGTGGTGCTGC
  TGCAGGGCGCGGAGCTCCTGCTGGCCGCCCGGCTACTCGGGGCCCTCGCTGCCCGCAGTGGGGCGGCGTA
  CGGCCCCGGAGCGCGCGGGGAGGACCGCGCTGGCCCCCAGAGCCCCAGCCCCGGCGCCCCGCCCGCTGCC
  AAACCCGCCCGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207457 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGERSPLLSQETAGQKPLSVHRPPTSGCLGPVPREDQAEAWGCSCCPPETKHQALSGTPKKGPAPSLS
  PGSSCVKYLIFLSNFPFSLLGLLALAIGLWGLAVKGSLGSDLGGPLPTDPMLGLALGGLVVSAASLAGCL
  GALCENTCLLRGFSGGILAFLVLEAVAGALVVALWGPLQDSLEHTLRVAIAHYQDDPDLRFLLDQVQLGL
  RCCGAASYQDWQQNLYFNCSSPGVQACSLPASCCIDPREDGASVNDQCGFGVLRLDADAAQRVVYLEGCG
  PPLRRWLRANLAASGGYAIAVVLLQGAELLLAARLLGALAARSGAAYGPGARGEDRAGPQSPSPGAPPAA
  KPARG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031945
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031945.2, NP_114151.2
• RefSeq Size: 1659 bp
• RefSeq ORF: 1068 bp
• Locus ID: 83882
• UniProt ID: Q9H1Z9
• Cytogenetics: 17q25.3
• Protein Families: Transmembrane
• MW: 36.5 kDa
• Gene Summary: Regulates maturation of the transmembrane metalloprotease ADAM10.[UniProtKB/Swiss-Prot Function]