WDTC1 (NM_015023) Human Tagged ORF Clone

CAT#: RC207453

WDTC1 (Myc-DDK-tagged)-Human WD and tetratricopeptide repeats 1 (WDTC1)

Product Images

Circular map for RC207453

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  Western blot validation of overexpression lysate (Cat# LY414858) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207453 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDTC1 protein (Cat# TP307453). The protein was produced from HEK293T cells transfected with WDTC1 cDNA clone (Cat# RC207453) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDTC1
• Synonyms: ADP; DCAF9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207453 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAAGTCAACATAACTAGAGACCTCATCCGTAGGCAGATCAAGGAGCGGGGTGCCCTGAGCTTTG
  AGCGGCGCTACCATGTCACTGACCCCTTTATCCGGCGGCTGGGCCTGGAAGCAGAGCTGCAGGGTCACTC
  AGGATGTGTCAACTGTCTGGAGTGGAATGAGAAAGGAGACTTGCTGGCCTCTGGTTCCGATGACCAGCAC
  ACGATTGTGTGGGACCCGCTGCACCACAAGAAGCTGCTCTCCATGCACACGGGACACACCGCAAATATCT
  TCTCTGTCAAGTTCCTGCCTCACGCTGGGGACCGCATCTTGATCACGGGGGCAGCCGACTCTAAGGTGCA
  TGTGCACGACCTGACAGTAAAGGAGACCATCCACATGTTTGGAGACCACACAAACCGGGTGAAGCGCATC
  GCCACAGCGCCCATGTGGCCCAACACATTCTGGAGTGCTGCTGAGGATGGGCTTATCCGCCAGTATGACC
  TTCGAGAGAACAGCAAACACTCGGAGGTGCTGATTGACCTGACAGAGTACTGTGGCCAGCTGGTGGAGGC
  CAAGTGCCTCACTGTCAACCCCCAGGACAACAACTGCCTGGCAGTTGGGGCCAGCGGGCCCTTCGTGAGG
  CTCTATGACATCCGCATGATCCATAACCACAGAAAGAGCATGAAGCAGAGCCCTTCAGCGGGTGTGCACA
  CCTTCTGTGACCGGCAGAAACCCCTTCCGGACGGTGCAGCCCAGTATTACGTAGCAGGTCACCTGCCAGT
  GAAGCTTCCTGACTACAACAACCGTTTGAGAGTGCTGGTTGCCACCTATGTGACCTTCAGCCCCAATGGC
  ACAGAGCTACTAGTCAACATGGGGGGGGAACAGGTCTATTTGTTTGACTTGACTTACAAGCAGCGGCCGT
  ACACCTTCCTCTTGCCTAGAAAATGCCACTCCTCGGGGGTCCAGAATGGCAAGATGTCCACCAACGGTGT
  GTCCAACGGTGTGTCCAATGGCCTGCACCTTCATAGCAATGGCTTCCGGCTGCCGGAGAGTAGGGGACAT
  GTCAGCCCCCAAGTAGAGCTACCACCATACCTGGAGCGTGTGAAACAGCAAGCCAATGAGGCTTTTGCCT
  GCCAGCAGTGGACCCAAGCCATTCAGCTTTACAGCAAGGCTGTGCAGAGGGCCCCTCACAATGCCATGCT
  TTATGGAAACCGAGCAGCAGCCTACATGAAGCGCAAGTGGGATGGTGACCACTATGATGCCCTGAGGGAC
  TGCCTCAAGGCCATCTCCCTAAACCCATGCCACCTGAAGGCACACTTTCGCCTGGCCCGCTGCCTCTTTG
  AGCTCAAGTATGTGGCTGAAGCCCTGGAGTGCCTGGACGACTTCAAAGGGAAATTTCCGGAGCAGGCCCA
  CAGCAGCGCTTGTGATGCATTGGGCCGCGACATCACAGCTGCCCTCTTCTCTAAAAATGATGGTGAGGAG
  AAGAAGGGACCTGGTGGCGGCGCCCCAGTCCGCCTCCGCAGCACGAGCCGCAAGGACTCCATCTCAGAGG
  ATGAAATGGTGCTGCGGGAGCGAAGCTACGACTATCAGTTCCGCTACTGCGGCCACTGCAACACCACCAC
  GGATATCAAAGAGGCCAATTTCTTTGGCAGCAACGCTCAGTATATCGTCAGTGGCTCTGACGATGGCTCC
  TTCTTCATCTGGGAAAAGGAGACCACCAACCTGGTCCGTGTGCTCCAAGGGGATGAGTCCATTGTCAACT
  GCCTGCAGCCACACCCCAGCTACTGCTTCCTGGCCACCAGTGGCATCGATCCTGTTGTGCGGCTCTGGAA
  CCCCCGACCAGAGAGTGAAGACCTCACAGGCCGAGTCGTGGAAGATATGGAGGGTGCTTCACAGGCCAAC
  CAGCGGCGCATGAATGCAGACCCGTTGGAGGTGATGCTGCTCAACATGGGCTACCGGATCACGGGCCTGA
  GCAGTGGGGGTGCCGGGGCCTCTGATGATGAGGACAGCTCTGAGGGCCAGGTGCAGTGCCGGCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207453 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKVNITRDLIRRQIKERGALSFERRYHVTDPFIRRLGLEAELQGHSGCVNCLEWNEKGDLLASGSDDQH
  TIVWDPLHHKKLLSMHTGHTANIFSVKFLPHAGDRILITGAADSKVHVHDLTVKETIHMFGDHTNRVKRI
  ATAPMWPNTFWSAAEDGLIRQYDLRENSKHSEVLIDLTEYCGQLVEAKCLTVNPQDNNCLAVGASGPFVR
  LYDIRMIHNHRKSMKQSPSAGVHTFCDRQKPLPDGAAQYYVAGHLPVKLPDYNNRLRVLVATYVTFSPNG
  TELLVNMGGEQVYLFDLTYKQRPYTFLLPRKCHSSGVQNGKMSTNGVSNGVSNGLHLHSNGFRLPESRGH
  VSPQVELPPYLERVKQQANEAFACQQWTQAIQLYSKAVQRAPHNAMLYGNRAAAYMKRKWDGDHYDALRD
  CLKAISLNPCHLKAHFRLARCLFELKYVAEALECLDDFKGKFPEQAHSSACDALGRDITAALFSKNDGEE
  KKGPGGGAPVRLRSTSRKDSISEDEMVLRERSYDYQFRYCGHCNTTTDIKEANFFGSNAQYIVSGSDDGS
  FFIWEKETTNLVRVLQGDESIVNCLQPHPSYCFLATSGIDPVVRLWNPRPESEDLTGRVVEDMEGASQAN
  QRRMNADPLEVMLLNMGYRITGLSSGGAGASDDEDSSEGQVQCRPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015023
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015023.5
• RefSeq Size: 4816 bp
• RefSeq ORF: 2031 bp
• Locus ID: 23038
• UniProt ID: Q8N5D0
• Cytogenetics: 1p36.11
• Domains: TPR, WD40
• MW: 75.8 kDa
• Gene Summary: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.[UniProtKB/Swiss-Prot Function]