MPND (NM_032868) Human Tagged ORF Clone

CAT#: RC207440

MPND (Myc-DDK-tagged)-Human MPN domain containing (MPND), transcript variant 1

Product Images

Circular map for RC207440

• 

  Western blot validation of overexpression lysate (Cat# LY409901) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207440 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MPND protein (Cat# TP307440). The protein was produced from HEK293T cells transfected with MPND cDNA clone (Cat# RC207440) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPND
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207440 representing NM_032868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTCCGGAGCCGCTGTCCCCGGCGGGCGGTGCGGGCGAGGAGGCGCCGGAGGAGGACGAGGACG
  AAGCGGAGGCCGAGGACCCTGAGCGGCCGAATGCGGGAGCGGGCGGTGGACGCAGTGGCGGCGGCGGCAG
  TAGCGTCAGCGGAGGAGGCGGCGGCGGCGGGGCCGGAGCGGGGGGCTGCGGCGGGCCCGGGGGCGCGCTC
  ACCAGGCGCGCGGTCACACTGCGGGTGCTCCTCAAAGACGCGCTGCTGGAGCCTGGCGCCGGGGTGCTGT
  CCATCTACTACCTGGGGAAGAAGTTCCTGGGCGACCTGCAGCCAGACGGAAGGATCATGTGGCAGGAGAC
  CGGGCAGACCTTCAACTCACCCAGCGCCTGGGCCACCCACTGCAAGAAGCTGGTGAACCCTGCCAAGAAG
  TCGGGCTGTGGCTGGGCCTCTGTCAAGTACAAAGGCCAGAAACTGGACAAGTACAAGGCCACCTGGCTCC
  GGCTGCACCAGCTGCACACGCCTGCCACGGCTGCTGATGAGAGCCCAGCCAGTGAAGGGGAGGAGGAGGA
  GTTGCTGATGGAAGAGGAGGAGGAGGACGTTCTGGCGGGGGTCTCAGCAGAGGACAAGAGTCGGAGACCA
  CTGGGGAAGAGCCCTTCAGAGCCTGCCCACCCGGAGGCCACAACCCCAGGGAAGCGGGTGGACAGCAAGA
  TCCGGGTTCCGGTCCGCTACTGCATGCTGGGCAGCCGCGACTTGGCCAGGAACCCCCACACCCTGGTGGA
  AGTAACATCCTTTGCAGCCATCAACAAGTTCCAGCCGTTCAACGTGGCTGTTTCTAGCAACGTGCTGTTC
  CTGCTGGACTTCCACAGTCACCTGACACGGAGTGAGGTCGTGGGTTACCTGGGGGGCCGCTGGGACGTCA
  ACAGCCAGATGCTGACGGTGCTCAGAGCCTTCCCTTGTCGGAGCCGGCTCGGGGACGCAGAGACTGCAGC
  TGCCATCGAAGAGGAGATCTACCAGAGCCTGTTCCTGCGGGGCCTGTCCCTGGTGGGCTGGTACCACAGC
  CACCCACACAGCCCGGCGCTGCCATCTCTGCAGGACATCGACGCACAGATGGACTACCAGCTGCGGCTGC
  AGGGCTCCAGCAATGGCTTCCAGCCCTGCCTCGCCCTGCTCTGCTCCCCTTACTATTCTGGCAACCCAGG
  CCCCGAGTCCAAGATCTCGCCTTTCTGGGTGATGCCTCCTCCCGAGATGCTGCTGGTGGAGTTCTACAAG
  GGTTCCCCTGACCTCGTGAGGCTCCAGGAACCCTGGAGCCAGGAGCACACCTACCTCGACAAGCTTAAGA
  TCTCCTTGGCCAGCAGGACGCCCAAGGACCAGAGCCTGTGTCACGTCCTGGAACAGGTGTGCGGCGTCCT
  CAAGCAGGGGAGC
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207440 representing NM_032868
  Red=Cloning site Green=Tags(s)
  
  MAAPEPLSPAGGAGEEAPEEDEDEAEAEDPERPNAGAGGGRSGGGGSSVSGGGGGGGAGAGGCGGPGGAL
  TRRAVTLRVLLKDALLEPGAGVLSIYYLGKKFLGDLQPDGRIMWQETGQTFNSPSAWATHCKKLVNPAKK
  SGCGWASVKYKGQKLDKYKATWLRLHQLHTPATAADESPASEGEEEELLMEEEEEDVLAGVSAEDKSRRP
  LGKSPSEPAHPEATTPGKRVDSKIRVPVRYCMLGSRDLARNPHTLVEVTSFAAINKFQPFNVAVSSNVLF
  LLDFHSHLTRSEVVGYLGGRWDVNSQMLTVLRAFPCRSRLGDAETAAAIEEEIYQSLFLRGLSLVGWYHS
  HPHSPALPSLQDIDAQMDYQLRLQGSSNGFQPCLALLCSPYYSGNPGPESKISPFWVMPPPEMLLVEFYK
  GSPDLVRLQEPWSQEHTYLDKLKISLASRTPKDQSLCHVLEQVCGVLKQGS
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032868
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032868.3
• RefSeq Size: 1530 bp
• RefSeq ORF: 1416 bp
• Locus ID: 84954
• UniProt ID: Q8N594
• Cytogenetics: 19p13.3
• Domains: JAB_MPN
• Protein Families: Druggable Genome, Transcription Factors
• MW: 50.5 kDa
• Gene Summary: Probable protease.[UniProtKB/Swiss-Prot Function]