DARS2 (NM_018122) Human Tagged ORF Clone

CAT#: RC207360

DARS2 (Myc-DDK-tagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC207360

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  Western blot validation of overexpression lysate (Cat# LY413308) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207360 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DARS2 protein (Cat# TP307360). The protein was produced from HEK293T cells transfected with DARS2 cDNA clone (Cat# RC207360) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DARS2
• Synonyms: ASPRS; LBSL; MT-ASPRS; mtAspRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207360 representing NM_018122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAAC
  CGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTT
  TGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA
  TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCA
  TTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCA
  AGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAA
  ATCAAAGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGA
  AGAAAACAGAGGCTCTTCGGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCT
  GCGACTGAGGTCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA
  GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCCAGGGAACCTG
  GAAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGCGGTTTAGACAG
  ATATTTTCAGGTTGCCCGATGTTATCGAGATGAAGGTTCAAGACCAGACAGACAGCCTGAGTTTACTCAG
  ATTGACATAGAGATGTCATTTGTAGACCAGACTGGGATCCAGAGTTTAATTGAGGGTTTGCTCCAGTATT
  CCTGGCCCAATGACAAAGATCCTGTGGTTGTTCCTTTTCCTACTATGACTTTTGCTGAGGTGCTGGCCAC
  CTATGGAACTGATAAACCTGACACTCGCTTTGGAATGAAGATTATAGATATCAGTGATGTGTTTAGAAAC
  ACAGAGATTGGATTTCTTCAAGATGCACTTAGTAAGCCCCATGGAACTGTGAAAGCCATATGTATCCCTG
  AAGGAGCAAAATACTTAAAAAGGAAAGACATTGAATCCATTAGAAACTTTGCAGCTGACCATTTTAATCA
  GGAAATCTTACCTGTATTCCTTAACGCCAATAGAAACTGGAATTCTCCAGTTGCTAATTTCATAATGGAG
  TCACAAAGACTGGAATTAATCAGACTAATGGAGACCCAAGAGGAAGATGTGGTCCTACTAACTGCTGGAG
  AGCACAATAAAGCATGCTCTTTGTTAGGAAAATTACGACTGGAATGTGCTGACCTTCTAGAAACAAGAGG
  AGTGGTGCTCCGTGACCCCACTCTGTTCTCTTTCCTTTGGGTGGTAGATTTCCCACTCTTCCTGCCCAAG
  GAGGAAAATCCCAGAGAGCTGGAATCGGCCCACCACCCATTTACTGCTCCCCACCCCAGTGACATACATC
  TCCTGTACACTGAGCCCAAAAAGGCCCGTAGCCAACACTATGACTTGGTTTTAAATGGCAATGAAATAGG
  AGGTGGTTCAATTCGAATTCACAATGCAGAGCTGCAGCGTTATATCCTGGCAACCTTACTAAAGGAGGAT
  GTGAAAATGCTCTCCCATCTGCTCCAGGCTTTAGATTATGGGGCACCCCCTCATGGAGGAATTGCCTTAG
  GGTTAGACAGACTGATATGCCTTGTCACTGGATCTCCAAGCATCAGAGATGTCATAGCCTTCCCAAAGTC
  CTTCCGGGGACATGACCTCATGAGCAATACCCCAGATTCTGTCCCTCCTGAGGAACTGAAGCCCTATCAT
  ATCCGAGTCTCCAAGCCAACAGACTCCAAAGCAGAAAGAGCTCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207360 representing NM_018122
  Red=Cloning site Green=Tags(s)
  
  MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCG
  WIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIE
  IKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDI
  ETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQ
  IDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRN
  TEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIME
  SQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPK
  EENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKED
  VKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYH
  IRVSKPTDSKAERAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018122
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018122.5
• RefSeq Size: 3187 bp
• RefSeq ORF: 1938 bp
• Locus ID: 55157
• UniProt ID: Q6PI48
• Cytogenetics: 1q25.1
• Domains: GAD, tRNA-synt_2
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 73.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]