FIG4 (NM_014845) Human Tagged ORF Clone

CAT#: RC207192

FIG4 (Myc-DDK-tagged)-Human FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae) (FIG4)

Product Images

Circular map for RC207192

• 

  Western blot validation of overexpression lysate (Cat# LY414969) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207192 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FIG4 protein (Cat# TP307192). The protein was produced from HEK293T cells transfected with FIG4 cDNA clone (Cat# RC207192) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FIG4
• Synonyms: ALS11; BTOP; CMT4J; dJ249I4.1; KIAA0274; SAC3; YVS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207192 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCACGGCCGCCGCCCCCATCATCAGCTCGGTCCAGAAGCTGGTTCTGTATGAGACTAGAGCTAGAT
  ACTTTCTAGTTGGGAGCAATAATGCAGAAACGAAATATCGTGTCTTGAAGATTGATAGAACAGAACCAAA
  AGATTTGGTCATAATTGATGACAGGCATGTCTATACTCAACAAGAAGTAAGGGAACTTCTTGGCCGCTTG
  GATCTTGGAAATAGAACAAAGATGGGACAGAAAGGATCCTCGGGCTTATTTCGAGCGGTTTCAGCTTTTG
  GTGTTGTGGGTTTTGTCAGGTTCTTAGAAGGCTATTATATTGTGTTAATAACTAAAAGGAGGAAGATGGC
  GGATATTGGAGGTCATGCAATCTATAAGGTCGAAGATACAAATATGATCTATATACCCAATGATTCTGTA
  CGGGTTACTCATCCTGATGAAGCTAGGTATCTACGAATATTTCAAAATGTGGACCTATCTAGCAATTTTT
  ACTTTAGTTACAGCTATGATTTGTCCCACTCACTTCAATATAATCTCACTGTCTTGCGAATGCCCCTGGA
  GATGTTAAAGTCAGAAATGACCCAGAATCGCCAAGAGAGCTTTGACATCTTTGAAGATGAAGGATTAATT
  ACACAAGGTGGAAGCGGGGTATTTGGGATCTGTAGTGAGCCTTATATGAAATATGTATGGAATGGTGAAC
  TTCTGGATATAATTAAAAGTACTGTGCATCGTGACTGGCTTTTGTATATTATTCATGGGTTCTGTGGGCA
  GTCAAAGCTGTTGATCTATGGACGACCAGTGTATGTCACTCTAATAGCTAGAAGATCCAGTAAATTTGCT
  GGCACCCGTTTTCTTAAAAGAGGTGCAAACTGTGAGGGTGATGTTGCAAATGAAGTGGAGACTGAACAAA
  TACTCTGCGATGCTTCTGTGATGTCTTTCACTGCAGGAAGTTATTCTTCATATGTACAAGTTAGAGGATC
  TGTGCCCTTATACTGGTCTCAGGACATTTCAACTATGATGCCTAAACCACCTATTACATTGGATCAGGCA
  GATCCATTTGCACATGTGGCTGCCCTTCACTTTGACCAGATGTTCCAGAGGTTTGGCTCTCCCATCATCA
  TCTTGAATTTAGTGAAGGAACGAGAGAAAAGAAAGCATGAAAGAATTCTGAGTGAAGAACTTGTTGCTGC
  TGTGACCTATCTCAACCAATTTTTGCCTCCTGAGCACACTATTGTTTATATTCCCTGGGACATGGCCAAG
  TATACCAAAAGCAAGCTGTGTAATGTTCTTGATCGACTAAATGTGATTGCAGAAAGTGTGGTGAAGAAAA
  CAGGTTTCTTTGTAAACCGCCCTGATTCTTACTGTAGCATTTTGCGGCCAGATGAAAAGTGGAATGAACT
  AGGAGGATGTGTGATTCCCACTGGTCGCCTGCAGACTGGCATCCTTCGAACCAACTGTGTGGACTGTTTA
  GATCGCACCAACACAGCACAGTTTATGGTGGGAAAATGTGCTCTGGCCTATCAGCTGTATTCACTGGGAC
  TGATTGACAAACCTAATCTACAGTTTGATACAGATGCAGTTAGGTTATTTGAGGAACTCTATGAAGATCA
  TGGTGATACCCTATCCCTTCAGTATGGTGGTTCTCAACTTGTTCATCGTGTGAAAACCTACAGAAAGATA
  GCACCATGGACCCAGCACTCCAAAGACATCATGCAAACCCTGTCTAGATATTACAGCAATGCTTTTTCAG
  ATGCCGATAGACAAGATTCCATTAATCTCTTCCTGGGAGTTTTCCATCCCACTGAAGGGAAACCTCATCT
  CTGGGAGCTCCCAACAGATTTTTATTTGCATCACAAAAATACCATGAGACTTTTGCCAACAAGAAGAAGT
  TATACTTACTGGTGGACACCAGAGGTGATAAAGCATTTACCATTGCCCTATGATGAAGTTATCTGTGCTG
  TGAACTTAAAGAAGTTGATAGTGAAGAAATTCCACAAATATGAAGAAGAGATTGATATCCACAATGAGTT
  CTTTCGGCCATATGAGTTGAGCAGCTTTGATGATACCTTTTGCTTGGCTATGACAAGCTCAGCACGTGAC
  TTTATGCCTAAGACCGTTGGAATTGATCCAAGTCCATTTACTGTGCGTAAACCAGATGAAACTGGAAAAT
  CAGTATTGGGAAACAAAAGCAATAGAGAAGAAGCTGTATTACAGCGGAAAACGGCAGCCAGCGCCCCGCC
  GCCACCCAGCGAGGAGGCTGTGTCCAGCAGCTCTGAGGATGACTCTGGGACTGATCGGGAAGAAGAGGGC
  TCTGTGTCTCAGCGCTCCACTCCCGTGAAGATGACTGATGCAGGAGACAGTGCCAAAGTGACCGAGAATG
  TGGTCCAACCCATGAAGGAGCTATATGGAATTAACCTCTCAGATGGCCTCTCAGAAGAAGATTTCTCCAT
  TTATTCAAGATTTGTTCAGCTGGGGCAGAGTCAACATAAACAAGACAAGAATAGCCAGCAGCCCTGTTCT
  AGGTGCTCAGATGGAGTTATAAAACTAACACCCATCTCGGCTTTCTCGCAAGATAACATCTATGAAGTTC
  AGCCCCCAAGAGTAGACAGAAAATCTACAGAGATCTTCCAAGCCCACATCCAGGCCAGCCAAGGTATCAT
  GCAGCCCCTAGGAAAAGAGGACTCCTCCATGTACCGAGAGTACATCAGGAACCGCTACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207192 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPTAAAPIISSVQKLVLYETRARYFLVGSNNAETKYRVLKIDRTEPKDLVIIDDRHVYTQQEVRELLGRL
  DLGNRTKMGQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKVEDTNMIYIPNDSV
  RVTHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSEMTQNRQESFDIFEDEGLI
  TQGGSGVFGICSEPYMKYVWNGELLDIIKSTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSKFA
  GTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLYWSQDISTMMPKPPITLDQA
  DPFAHVAALHFDQMFQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAK
  YTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGCVIPTGRLQTGILRTNCVDCL
  DRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKI
  APWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMRLLPTRRS
  YTYWWTPEVIKHLPLPYDEVICAVNLKKLIVKKFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARD
  FMPKTVGIDPSPFTVRKPDETGKSVLGNKSNREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREEEG
  SVSQRSTPVKMTDAGDSAKVTENVVQPMKELYGINLSDGLSEEDFSIYSRFVQLGQSQHKQDKNSQQPCS
  RCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDSSMYREYIRNRYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014845
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014845.3
• RefSeq Size: 3123 bp
• RefSeq ORF: 2724 bp
• Locus ID: 9896
• UniProt ID: Q92562
• Cytogenetics: 6q21
• Domains: Syja_N
• MW: 103.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J. [provided by RefSeq, Jul 2008]