SLC44A2 (NM_020428) Human Tagged ORF Clone

CAT#: RC207181

SLC44A2 (Myc-DDK-tagged)-Human solute carrier family 44, member 2 (SLC44A2), transcript variant 1

Product Images

Circular map for RC207181

• 

  Western blot validation of overexpression lysate (Cat# LY412479) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207181 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC44A2
• Synonyms: CTL2; PP1292
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207181 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACGAGCGGCCCCACTACTACGGGAAACACGGAACGCCACAGAAGTATGATCCCACTTTCAAAG
  GACCCATTTACAATAGGGGCTGCACGGATATCATATGCTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA
  CGTGGCTGTAGGCATCATAGCCTGGACTCATGGAGACCCTCGAAAGGTGATCTACCCCACTGATAGCCGG
  GGCGAGTTCTGCGGGCAGAAGGGCACAAAAAACGAGAACAAACCCTATCTGTTTTATTTCAACATTGTGA
  AATGTGCCAGCCCCCTGGTTCTGCTGGAATTCCAATGTCCCACTCCCCAGATCTGCGTGGAAAAATGCCC
  CGACCGCTACCTCACGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAGTACTATAAGCAGTTCTGTGTT
  CCTGGCTTCAAGAACAATAAAGGAGTGGCTGAGGTGCTTCGAGATGGTGACTGCCCTGCTGTCCTCATCC
  CCAGCAAACCCTTGGCCCGGAGATGCTTCCCCGCTATCCACGCCTACAAGGGTGTCCTGATGGTGGGCAA
  TGAGACGACCTATGAGGATGGGCATGGCTCCCGGAAAAACATCACAGACCTGGTGGAGGGCGCCAAGAAA
  GCCAATGGAGTCCTAGAGGCGCGGCAACTCGCCATGCGCATATTTGAAGATTACACCGTCTCTTGGTACT
  GGATTATCATAGGCCTGGTCATTGCCATGGCGATGAGCCTCCTGTTCATCATCCTGCTTCGCTTCCTGGC
  TGGTATTATGGTCTGGGTGATGATCATCATGGTGATTCTGGTGCTGGGCTACGGAATATTTCACTGCTAC
  ATGGAGTACTCCCGACTGCGTGGTGAGGCCGGCTCTGATGTCTCTTTGGTGGACCTCGGCTTTCAGACGG
  ATTTCCGGGTGTACCTGCACTTACGGCAGACCTGGTTGGCCTTTATGATCATTCTGAGTATCCTTGAAGT
  CATTATCATCTTGCTGCTCATCTTTCTCCGGAAGAGAATTCTCATCGCGATTGCACTCATCAAAGAAGCC
  AGCAGGGCTGTGGGATACGTCATGTGCTCCTTGCTCTACCCACTGGTCACCTTCTTCTTGCTGTGCCTCT
  GCATCGCCTACTGGGCCAGCACTGCTGTCTTCCTGTCCACTTCCAACGAAGCGGTCTATAAGATCTTTGA
  TGACAGCCCCTGCCCATTTACTGCGAAAACCTGCAACCCAGAGACCTTCCCCTCCTCCAATGAGTCCCGC
  CAATGCCCCAATGCCCGTTGCCAGTTCGCCTTCTACGGTGGTGAGTCGGGCTACCACCGGGCCCTGCTGG
  GCCTGCAGATCTTCAATGCCTTCATGTTCTTCTGGTTGGCCAACTTCGTGCTGGCGCTGGGCCAGGTCAC
  GCTGGCCGGGGCCTTTGCCTCCTACTACTGGGCCCTGCGCAAGCCGGACGACCTGCCGGCCTTCCCGCTC
  TTCTCTGCCTTTGGCCGGGCGCTCAGGTACCACACAGGCTCCCTGGCCTTTGGCGCGCTCATCCTGGCCA
  TTGTGCAGATCATCCGTGTGATACTCGAGTACCTGGATCAGCGGCTGAAAGCTGCAGAGAACAAGTTTGC
  CAAGTGCCTCATGACCTGTCTCAAATGCTGCTTCTGGTGCCTGGAGAAGTTCATCAAATTCCTTAATAGG
  AATGCCTACATCATGATTGCCATCTACGGCACCAATTTCTGCACCTCGGCCAGGAATGCCTTCTTCCTGC
  TCATGAGAAACATCATCAGAGTGGCTGTCCTGGATAAAGTTACTGACTTCCTCTTCCTGTTGGGCAAACT
  TCTGATCGTTGGTAGTGTGGGGATCCTGGCTTTCTTCTTCTTCACCCACCGTATCAGGATCGTGCAGGAT
  ACAGCACCACCCCTCAATTATTACTGGGTTCCTATACTGACGGTGATCGTTGGCTCCTACTTGATTGCAC
  ACGGTTTCTTCAGCGTCTATGGCATGTGTGTGGACACGCTGTTCCTCTGCTTCTTGGAGGACCTGGAGAG
  GAATGACGGCTCGGCCGAGAGGCCTTACTTCATGTCTTCCACCCTCAAGAAACCCTTGAACAAGACCAAC
  AAGAAGGCAGCGGAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207181 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDERPHYYGKHGTPQKYDPTFKGPIYNRGCTDIICCVFLLLAIVGYVAVGIIAWTHGDPRKVIYPTDSR
  GEFCGQKGTKNENKPYLFYFNIVKCASPLVLLEFQCPTPQICVEKCPDRYLTYLNARSSRDFEYYKQFCV
  PGFKNNKGVAEVLRDGDCPAVLIPSKPLARRCFPAIHAYKGVLMVGNETTYEDGHGSRKNITDLVEGAKK
  ANGVLEARQLAMRIFEDYTVSWYWIIIGLVIAMAMSLLFIILLRFLAGIMVWVMIIMVILVLGYGIFHCY
  MEYSRLRGEAGSDVSLVDLGFQTDFRVYLHLRQTWLAFMIILSILEVIIILLLIFLRKRILIAIALIKEA
  SRAVGYVMCSLLYPLVTFFLLCLCIAYWASTAVFLSTSNEAVYKIFDDSPCPFTAKTCNPETFPSSNESR
  QCPNARCQFAFYGGESGYHRALLGLQIFNAFMFFWLANFVLALGQVTLAGAFASYYWALRKPDDLPAFPL
  FSAFGRALRYHTGSLAFGALILAIVQIIRVILEYLDQRLKAAENKFAKCLMTCLKCCFWCLEKFIKFLNR
  NAYIMIAIYGTNFCTSARNAFFLLMRNIIRVAVLDKVTDFLFLLGKLLIVGSVGILAFFFFTHRIRIVQD
  TAPPLNYYWVPILTVIVGSYLIAHGFFSVYGMCVDTLFLCFLEDLERNDGSAERPYFMSSTLKKPLNKTN
  KKAAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020428
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020428.2, NP_065161.2
• RefSeq Size: 3434 bp
• RefSeq ORF: 2121 bp
• Locus ID: 57153
• UniProt ID: Q8IWA5
• Cytogenetics: 19p13.2
• Domains: DUF580
• Protein Families: Transmembrane
• MW: 80.1 kDa
• Gene Summary: Isoform 1, but not isoform 3, exhibits some choline transporter activity.[UniProtKB/Swiss-Prot Function]