MCM6 (NM_005915) Human Tagged ORF Clone

CAT#: RC207097

MCM6 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 6 (MCM6)

Product Images

Circular map for RC207097

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCM6 (Cat# RC207097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM6(Cat# TA590213). Positive lysates LY401786 (100ug) and LC401786 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401786) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207097 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MCM6 protein (Cat# TP307097). The protein was produced from HEK293T cells transfected with MCM6 cDNA clone (Cat# RC207097) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCM6
• Synonyms: MCG40308; Mis5; P105MCM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207097 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCGCGGCGGCAGCGGAGCCGGGCGCCGGCAGCCAGCACCTGGAGGTCCGCGACGAGGTGGCCG
  AGAAGTGCCAGAAACTGTTCCTGGACTTCTTGGAGGAGTTTCAGAGCAGCGATGGAGAAATTAAATACTT
  GCAATTAGCAGAGGAACTGATTCGTCCTGAGAGAAACACATTGGTTGTGAGTTTTGTGGACCTGGAACAA
  TTTAACCAGCAACTTTCCACCACCATTCAAGAGGAGTTCTATAGAGTTTACCCTTACCTGTGTCGGGCCT
  TGAAAACATTCGTCAAAGACCGTAAAGAGATCCCTCTTGCCAAGGATTTTTATGTTGCATTCCAAGACCT
  GCCTACCAGACACAAGATTCGAGAGCTCACCTCATCCAGAATTGGTTTGCTCACTCGCATCAGTGGGCAG
  GTGGTGCGGACTCACCCAGTTCACCCAGAGCTTGTGAGCGGAACTTTTCTGTGCTTGGACTGTCAGACAG
  TGATCAGGGATGTAGAACAGCAGTTCAAATACACACAGCCAAACATCTGCCGAAATCCAGTTTGTGCCAA
  CAGGAGGAGATTCTTACTGGATACAAATAAATCAAGATTTGTTGATTTTCAAAAGGTTCGTATTCAAGAG
  ACCCAAGCTGAGCTTCCTCGAGGGAGTATCCCCCGCAGTTTAGAAGTAATTTTAAGGGCTGAAGCTGTGG
  AATCAGCTCAAGCTGGTGACAAGTGTGACTTTACAGGGACACTGATTGTTGTGCCTGACGTCTCCAAGCT
  TAGCACACCAGGAGCACGTGCAGAAACTAATTCCCGTGTCAGTGGTGTTGATGGATATGAGACAGAAGGC
  ATTCGAGGACTCCGGGCCCTTGGTGTTAGGGACCTTTCTTATAGGCTGGTCTTTCTTGCCTGCTGTGTTG
  CGCCAACCAACCCAAGGTTTGGGGGGAAAGAGCTCAGAGATGAGGAACAGACAGCTGAGAGCATTAAGAA
  CCAAATGACTGTGAAAGAATGGGAGAAAGTGTTTGAGATGAGTCAAGATAAAAATCTATACCACAATCTT
  TGTACCAGCCTGTTCCCTACTATACATGGCAATGATGAAGTAAAACGGGGTGTCCTGCTGATGCTCTTTG
  GTGGCGTTCCAAAGACAACAGGAGAAGGGACCTCTCTTCGAGGGGACATAAATGTTTGCATTGTTGGTGA
  CCCAAGTACAGCTAAGAGCCAATTTCTCAAGCACGTGGAGGAGTTCAGCCCCAGAGCTGTCTACACCAGT
  GGTAAAGCGTCCAGTGCTGCTGGCTTAACAGCAGCTGTTGTGAGAGATGAAGAATCTCATGAGTTTGTCA
  TTGAGGCTGGAGCTTTGATGTTGGCTGATAATGGTGTGTGTTGTATTGATGAATTTGATAAGATGGACGT
  GCGGGATCAAGTTGCTATTCATGAAGCTATGGAACAGCAGACCATATCCATCACTAAAGCAGGAGTGAAG
  GCTACTCTGAACGCCCGGACGTCCATTTTGGCAGCAGCAAACCCAATCAGTGGACACTATGACAGATCAA
  AATCATTGAAACAGAATATAAATTTGTCAGCTCCCATCATGTCCCGATTCGATCTCTTCTTTATCCTTGT
  GGATGAATGTAATGAGGTTACAGATTATGCCATTGCCAGGCGCATAGTAGATTTGCATTCAAGAATTGAG
  GAATCAATTGATCGTGTCTATTCCCTCGATGATATCAGAAGATATCTTCTCTTTGCAAGACAGTTTAAAC
  CCAAGATTTCCAAAGAGTCAGAGGACTTCATTGTGGAGCAATATAAACATCTCCGCCAGAGAGATGGTTC
  TGGAGTGACCAAGTCTTCATGGAGGATTACAGTGCGACAGCTTGAGAGCATGATTCGTCTCTCTGAAGCT
  ATGGCTCGGATGCACTGCTGTGATGAGGTCCAACCTAAACATGTGAAGGAAGCTTTCCGGTTACTGAATA
  AATCAATCATCCGTGTGGAAACACCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGGTAGA
  TGAGGGTGCCGGTGGCATCAATGGTCATGCTGACAGCCCTGCTCCTGTGAACGGGATCAATGGCTACAAT
  GAAGACATAAATCAAGAGTCTGCTCCCAAAGCCTCCTTAAGGCTGGGCTTCTCTGAGTACTGCCGAATCT
  CTAACCTTATTGTGCTTCACCTCAGAAAGGTGGAAGAAGAAGAGGACGAGTCAGCATTAAAGAGGAGCGA
  GCTTGTTAACTGGTACTTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAACTTATAAATAAAAAA
  AGAATCATAGAGAAAGTTATTCATCGACTCACACACTATGATCATGTTCTAATTGAGCTCACCCAGGCTG
  GATTGAAAGGCTCCACAGAGGGAAGTGAGAGCTATGAAGAAGATCCCTACTTGGTAGTTAACCCTAACTA
  CTTGCTCGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207097 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQ
  FNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQ
  VVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQE
  TQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEG
  IRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNL
  CTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
  GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVK
  ATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIE
  ESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEA
  MARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYN
  EDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKK
  RIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005915
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005915.3
• RefSeq Size: 3791 bp
• RefSeq ORF: 2466 bp
• Locus ID: 4175
• UniProt ID: Q14566
• Cytogenetics: 2q21.3
• Domains: MCM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• MW: 92.9 kDa
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]