NAT10 (NM_024662) Human Tagged ORF Clone

CAT#: RC207082

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NAT10 (Myc-DDK-tagged)-Human N-acetyltransferase 10 (GCN5-related) (NAT10), transcript variant 1



Reconstitution Protocol

USD 892.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NAT10
Synonyms ALP; Kre33; NET43
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207082 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCGGAAAAAGGTGGATAACCGAATCCGGATTCTCATTGAGAATGGAGTAGCTGAGCGGCAAAGAT
CTCTCTTTGTTGTAGTTGGGGATCGAGGAAAAGATCAGGTGGTAATACTTCATCACATGTTATCCAAAGC
AACTGTGAAGGCTCGGCCTTCAGTGCTGTGGTGTTATAAGAAAGAGCTGGGGTTTAGCAGTCACCGGAAG
AAAAGAATGCGACAGCTGCAGAAGAAAATAAAGAATGGAACACTGAACATAAAGCAGGACGACCCCTTTG
AACTCTTCATAGCAGCCACAAACATTCGCTACTGCTACTACAACGAGACCCACAAGATCCTGGGCAATAC
CTTCGGCATGTGTGTGCTGCAGGATTTTGAAGCCTTAACTCCAAACTTGCTGGCCAGGACTGTAGAAACA
GTGGAAGGTGGTGGGCTAGTGGTCATCCTCCTACGGACCATGAACTCACTCAAGCAATTGTACACAGTGA
CTATGGATGTGCATTCCAGGTACAGAACTGAGGCCCATCAGGATGTGGTGGGAAGATTTAATGAAAGGTT
TATTCTGTCTCTGGCCTCTTGTAAGAAGTGTCTCGTCATTGATGACCAGCTCAACATCCTGCCCATCTCC
TCCCACGTTGCCACCATGGAGGCCCTGCCTCCCCAGACTCCGGATGAGAGTCTTGGTCCTTCTGATCTGG
AGCTGAGGGAGTTGAAGGAGAGCTTGCAGGACACCCAGCCTGTGGGTGTGTTGGTGGACTGCTGTAAGAC
TCTAGACCAGGCCAAAGCTGTCTTGAAATTTATCGAGGGCATCTCTGAAAAGACCCTGAGGAGTACTGTT
GCACTCACAGCTGCTCGAGGACGGGGAAAATCTGCAGCCCTGGGATTGGCGATTGCTGGGGCGGTGGCAT
TTGGGTACTCCAATATCTTTGTTACCTCCCCAAGCCCTGATAACCTCCATACTCTGTTTGAATTTGTATT
TAAAGGATTTGATGCTCTGCAATATCAGGAACATCTGGATTATGAGATTATCCAGTCTCTAAATCCTGAA
TTTAACAAAGCAGTGATCAGAGTGAATGTATTTCGAGAACACAGGCAGACTATTCAGTATATACATCCTG
CAGATGCTGTGAAGCTGGGCCAGGCTGAACTAGTTGTGATTGATGAAGCTGCCGCCATCCCCCTCCCCTT
GGTGAAGAGCCTACTTGGCCCCTACCTTGTTTTCATGGCATCCACCATCAATGGCTATGAGGGCACTGGC
CGGTCACTGTCCCTCAAGCTAATTCAGCAGCTCCGTCAACAGAGCGCCCAGAGCCAGGTCAGCACCACTG
CTGAGAATAAGACCACGACGACAGCCAGATTGGCATCAGCGCGGACACTGCATGAGGTTTCCCTCCAGGA
GTCAATCCGATACGCCCCTGGGGATGCAGTGGAGAAGTGGCTGAATGACTTGCTGTGCCTGGATTGCCTC
AACATCACTCGGATAGTCTCAGGCTGCCCCTTGCCTGAAGCTTGTGAACTGTACTATGTTAATAGAGATA
CCCTCTTTTGCTACCACAAGGCCTCTGAAGTTTTCCTCCAACGGCTTATGGCCCTCTACGTGGCTTCTCA
CTACAAGAACTCTCCCAATGATCTCCAGATGCTCTCCGATGCACCTGCTCACCATCTCTTCTGCCTTCTG
CCTCCTGTGCCCCCCACCCAGAATGCCCTTCCAGAAGTGCTTGCTGTTATCCAGGTGTGCCTTGAAGGGG
AGATTTCTCGCCAGTCCATCTTGAACAGTCTGTCTCGAGGCAAGAAGGCTTCAGGGGACCTGATTCCATG
GACAGTGTCAGAACAGTTCCAAGATCCAGACTTTGGTGGTCTGTCTGGTGGAAGGGTCGTTCGCATTGCT
GTTCACCCAGATTATCAAGGGATGGGCTATGGCAGCCGTGCTCTGCAGCTGCTGCAGATGTACTATGAAG
GCAGGTTTCCTTGTCTGGAGGAAAAGGTCCTTGAGACACCACAGGAAATTCACACCGTAAGCAGCGAGGC
TGTCAGCTTGTTGGAAGAGGTCATCACTCCCCGGAAGGACCTGCCTCCTTTACTCCTCAAATTGAATGAG
AGGCCTGCCGAACGCCTGGATTACCTGGGTGTTTCCTATGGCTTGACCCCCAGGCTCCTCAAGTTCTGGA
AACGAGCTGGATTTGTTCCTGTTTATCTGAGACAGACCCCGAATGACCTGACCGGAGAGCACTCGTGCAT
CATGCTGAAGACGCTCACTGATGAGGATGAGGCTGACCAGGGAGGCTGGCTTGCAGCCTTCTGGAAAGAT
TTCCGACGGCGGTTCCTAGCCTTGCTCTCCTACCAGTTCAGTACCTTCTCTCCTTCCCTGGCTCTGAACA
TCATTCAGAACAGGAACATGGGGAAGCCAGCCCAGCCTGCCCTGAGCCGGGAGGAGCTGGAAGCACTCTT
CCTCCCCTATGACCTGAAGCGGCTGGAGATGTATTCACGGAATATGGTGGACTATCACCTCATCATGGAC
ATGATCCCGGCCATCTCTCGCATCTATTTCCTGAACCAGCTGGGGGACCTGGCCCTGTCTGCGGCTCAGT
CGGCTCTTCTCTTGGGGATTGGCCTGCAGCATAAGTCTGTGGACCAGCTGGAAAAGGAGATTGAGCTGCC
CTCGGGCCAGTTGATGGGACTTTTCAACCGGATCATCCGCAAAGTTGTGAAGCTATTTAATGAAGTTCAG
GAAAAGGCCATTGAGGAGCAGATGGTGGCAGCGAAGGATGTGGTCATGGAGCCCACGATGAAGACCCTCA
GTGACGACCTAGATGAAGCAGCAAAGGAATTTCAGGAGAAACACAAGAAGGAAGTAGGGAAGCTGAAGAG
CATGGACCTCTCTGAATACATAATCCGTGGGGACGATGAAGAGTGGAATGAAGTTTTGAACAAAGCTGGG
CCGAACGCCTCGATCATCAGCCTGAAAAGTGACAAGAAAAGGAAGTTAGAGGCCAAACAAGAACCCAAAC
AGAGCAAGAAGTTGAAGAACAGAGAGACAAAGAACAAAAAAGATATGAAACTGAAGCGGAAGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207082 protein sequence
Red=Cloning site Green=Tags(s)

MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK
KRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET
VEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPIS
SHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV
ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPE
FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG
RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL
NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL
PPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA
VHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNE
RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKD
FRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD
MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQ
EKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAG
PNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024662
ORF Size 3075 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024662.2
RefSeq Size 4058 bp
RefSeq ORF 3078 bp
Locus ID 55226
UniProt ID Q9H0A0
Cytogenetics 11p13
Domains DUF699
MW 115.7 kDa
Gene Summary The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization. [provided by RefSeq, Oct 2016]
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