NAT10 (NM_024662) Human Tagged ORF Clone

CAT#: RC207082

NAT10 (Myc-DDK-tagged)-Human N-acetyltransferase 10 (GCN5-related) (NAT10), transcript variant 1

Product Images

Circular map for RC207082

• 

  Western blot validation of overexpression lysate (Cat# LY411178) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207082 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NAT10 protein (Cat# TP307082). The protein was produced from HEK293T cells transfected with NAT10 cDNA clone (Cat# RC207082) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAT10
• Synonyms: ALP; Kre33; NET43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207082 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCGGAAAAAGGTGGATAACCGAATCCGGATTCTCATTGAGAATGGAGTAGCTGAGCGGCAAAGAT
  CTCTCTTTGTTGTAGTTGGGGATCGAGGAAAAGATCAGGTGGTAATACTTCATCACATGTTATCCAAAGC
  AACTGTGAAGGCTCGGCCTTCAGTGCTGTGGTGTTATAAGAAAGAGCTGGGGTTTAGCAGTCACCGGAAG
  AAAAGAATGCGACAGCTGCAGAAGAAAATAAAGAATGGAACACTGAACATAAAGCAGGACGACCCCTTTG
  AACTCTTCATAGCAGCCACAAACATTCGCTACTGCTACTACAACGAGACCCACAAGATCCTGGGCAATAC
  CTTCGGCATGTGTGTGCTGCAGGATTTTGAAGCCTTAACTCCAAACTTGCTGGCCAGGACTGTAGAAACA
  GTGGAAGGTGGTGGGCTAGTGGTCATCCTCCTACGGACCATGAACTCACTCAAGCAATTGTACACAGTGA
  CTATGGATGTGCATTCCAGGTACAGAACTGAGGCCCATCAGGATGTGGTGGGAAGATTTAATGAAAGGTT
  TATTCTGTCTCTGGCCTCTTGTAAGAAGTGTCTCGTCATTGATGACCAGCTCAACATCCTGCCCATCTCC
  TCCCACGTTGCCACCATGGAGGCCCTGCCTCCCCAGACTCCGGATGAGAGTCTTGGTCCTTCTGATCTGG
  AGCTGAGGGAGTTGAAGGAGAGCTTGCAGGACACCCAGCCTGTGGGTGTGTTGGTGGACTGCTGTAAGAC
  TCTAGACCAGGCCAAAGCTGTCTTGAAATTTATCGAGGGCATCTCTGAAAAGACCCTGAGGAGTACTGTT
  GCACTCACAGCTGCTCGAGGACGGGGAAAATCTGCAGCCCTGGGATTGGCGATTGCTGGGGCGGTGGCAT
  TTGGGTACTCCAATATCTTTGTTACCTCCCCAAGCCCTGATAACCTCCATACTCTGTTTGAATTTGTATT
  TAAAGGATTTGATGCTCTGCAATATCAGGAACATCTGGATTATGAGATTATCCAGTCTCTAAATCCTGAA
  TTTAACAAAGCAGTGATCAGAGTGAATGTATTTCGAGAACACAGGCAGACTATTCAGTATATACATCCTG
  CAGATGCTGTGAAGCTGGGCCAGGCTGAACTAGTTGTGATTGATGAAGCTGCCGCCATCCCCCTCCCCTT
  GGTGAAGAGCCTACTTGGCCCCTACCTTGTTTTCATGGCATCCACCATCAATGGCTATGAGGGCACTGGC
  CGGTCACTGTCCCTCAAGCTAATTCAGCAGCTCCGTCAACAGAGCGCCCAGAGCCAGGTCAGCACCACTG
  CTGAGAATAAGACCACGACGACAGCCAGATTGGCATCAGCGCGGACACTGCATGAGGTTTCCCTCCAGGA
  GTCAATCCGATACGCCCCTGGGGATGCAGTGGAGAAGTGGCTGAATGACTTGCTGTGCCTGGATTGCCTC
  AACATCACTCGGATAGTCTCAGGCTGCCCCTTGCCTGAAGCTTGTGAACTGTACTATGTTAATAGAGATA
  CCCTCTTTTGCTACCACAAGGCCTCTGAAGTTTTCCTCCAACGGCTTATGGCCCTCTACGTGGCTTCTCA
  CTACAAGAACTCTCCCAATGATCTCCAGATGCTCTCCGATGCACCTGCTCACCATCTCTTCTGCCTTCTG
  CCTCCTGTGCCCCCCACCCAGAATGCCCTTCCAGAAGTGCTTGCTGTTATCCAGGTGTGCCTTGAAGGGG
  AGATTTCTCGCCAGTCCATCTTGAACAGTCTGTCTCGAGGCAAGAAGGCTTCAGGGGACCTGATTCCATG
  GACAGTGTCAGAACAGTTCCAAGATCCAGACTTTGGTGGTCTGTCTGGTGGAAGGGTCGTTCGCATTGCT
  GTTCACCCAGATTATCAAGGGATGGGCTATGGCAGCCGTGCTCTGCAGCTGCTGCAGATGTACTATGAAG
  GCAGGTTTCCTTGTCTGGAGGAAAAGGTCCTTGAGACACCACAGGAAATTCACACCGTAAGCAGCGAGGC
  TGTCAGCTTGTTGGAAGAGGTCATCACTCCCCGGAAGGACCTGCCTCCTTTACTCCTCAAATTGAATGAG
  AGGCCTGCCGAACGCCTGGATTACCTGGGTGTTTCCTATGGCTTGACCCCCAGGCTCCTCAAGTTCTGGA
  AACGAGCTGGATTTGTTCCTGTTTATCTGAGACAGACCCCGAATGACCTGACCGGAGAGCACTCGTGCAT
  CATGCTGAAGACGCTCACTGATGAGGATGAGGCTGACCAGGGAGGCTGGCTTGCAGCCTTCTGGAAAGAT
  TTCCGACGGCGGTTCCTAGCCTTGCTCTCCTACCAGTTCAGTACCTTCTCTCCTTCCCTGGCTCTGAACA
  TCATTCAGAACAGGAACATGGGGAAGCCAGCCCAGCCTGCCCTGAGCCGGGAGGAGCTGGAAGCACTCTT
  CCTCCCCTATGACCTGAAGCGGCTGGAGATGTATTCACGGAATATGGTGGACTATCACCTCATCATGGAC
  ATGATCCCGGCCATCTCTCGCATCTATTTCCTGAACCAGCTGGGGGACCTGGCCCTGTCTGCGGCTCAGT
  CGGCTCTTCTCTTGGGGATTGGCCTGCAGCATAAGTCTGTGGACCAGCTGGAAAAGGAGATTGAGCTGCC
  CTCGGGCCAGTTGATGGGACTTTTCAACCGGATCATCCGCAAAGTTGTGAAGCTATTTAATGAAGTTCAG
  GAAAAGGCCATTGAGGAGCAGATGGTGGCAGCGAAGGATGTGGTCATGGAGCCCACGATGAAGACCCTCA
  GTGACGACCTAGATGAAGCAGCAAAGGAATTTCAGGAGAAACACAAGAAGGAAGTAGGGAAGCTGAAGAG
  CATGGACCTCTCTGAATACATAATCCGTGGGGACGATGAAGAGTGGAATGAAGTTTTGAACAAAGCTGGG
  CCGAACGCCTCGATCATCAGCCTGAAAAGTGACAAGAAAAGGAAGTTAGAGGCCAAACAAGAACCCAAAC
  AGAGCAAGAAGTTGAAGAACAGAGAGACAAAGAACAAAAAAGATATGAAACTGAAGCGGAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207082 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK
  KRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET
  VEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPIS
  SHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV
  ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPE
  FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG
  RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL
  NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL
  PPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA
  VHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNE
  RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKD
  FRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD
  MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQ
  EKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAG
  PNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024662
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024662.2
• RefSeq Size: 4058 bp
• RefSeq ORF: 3078 bp
• Locus ID: 55226
• UniProt ID: Q9H0A0
• Cytogenetics: 11p13
• Domains: DUF699
• MW: 115.7 kDa
• Gene Summary: The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization. [provided by RefSeq, Oct 2016]