p107 (RBL1) (NM_183404) Human Tagged ORF Clone

CAT#: RC207017

RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2

Product Images

Circular map for RC207017

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RBL1 (Cat# RC207017, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBL1 (Cat# TA811337)(1:500). Positive lysates LY403655 (100ug) and LC403655 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY403655) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207017 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: p107
• Synonyms: CP107; p107; PRB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207017 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCGCTACAGGCCC
  TGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGACGACTTTACTGCCATCCGAGG
  CAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCATGTTCATTATATGTTGCATGCCGCAAAAGC
  ATTATTCCCACGGTTGGAAAGGGTATCATGGAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAG
  CTAAATTAAGTTTAATACAATTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGA
  ATTTCGTGAACGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
  CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGCCGGAAGCAGA
  GGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTTTTTGTTTATACTAAGGGTAA
  TTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCATTTACTTCTATGCTGCTTGGATCTGATTTTT
  GCCAATGCGATTATGTGCCCAAATAGACAAGACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATT
  TTCATACTGCTGACTTTACGGCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGA
  TGGACTTCTCGTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
  AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAAGCAGTGAATA
  AGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATCTTTTTGGGAGCAGACGCAGA
  AGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACCCCATTAGGGAAACTGACAGCACAGGCTAAT
  GTGGAGTATAACCTTCAACAGCACTTTGAAAAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGAC
  GGAGATATTTACGAGAAAAAGAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTT
  ACAGAGTATTGTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
  CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAACACTATACTC
  AATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGACTAAAGCTGGCAGAAATTTT
  GTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACACGAAGACTTCATGGAATGGACATGTCAGTT
  CTTTTAGAGCAAGATATATTTCATCGTTCCTTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATA
  GCTCACCTCGTACTTTTCCTTGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTAT
  TGAGGTGGTGATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
  CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTTTCTGCAAACA
  AAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGAAATGGAGGAAATGTGCAGGG
  ACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCAAGAGTCAAGGAAGTTCGAACTGACAGTGGG
  AGTCTTCGAAGAGATATGCAACCATTGTCTCCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAG
  GGAGTGCTAAGAGAAGACTCTTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGA
  AGGAACAAAATTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
  ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATTCCATTACATG
  GTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAATACAAATCAGGAGTCCAAAGT
  CAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGTGCTTCTCCAAAACAGACCAATCTGACTAAA
  GCACAAGAGGTACATTCAACTGGAATAAACAGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAA
  AGGTCTATCATTTGGCAAGTGTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACG
  AAGGAAGATATGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
  GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACTTTTCAAGAAA
  TTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGAAGTGTTCTGCTGAAAAGTAT
  TCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGATGACTTTGAAATGATAGATTGTGACTTAGAA
  GATGCTACAAAAACACCTGACTGTTCCAGTGGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTT
  ACAATACAATATATGTAGGAAGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATAT
  GATGGATGCTCCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
  CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGCGCTCTGCTGT
  ACAAGTTCAATGGCAGCCCTTCTAAGGTAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207017 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFEDKPHAEGAAVVAAAGEALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVTHWLACSLYVACRKS
  IIPTVGKGIMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKYE
  PIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIF
  ANAIMCPNRQDLLNPSFKGLPSDFHTADFTASEEPPCIIAVLCELHDGLLVEAKGIKEHYFKPYISKLFD
  RKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTRDTPLGKLTAQAN
  VEYNLQQHFEKKRSFAPSTPLTGRRYLREKEAVITPVASATQSVSRLQSIVAGLKNAPSDQLINIFESCV
  RNPVENIMKILKGIGETFCQHYTQSTDEQPGSHIDFAVNRLKLAEILYYKILETVMVQETRRLHGMDMSV
  LLEQDIFHRSLMACCLEIVLFAYSSPRTFPWIIEVLNLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEE
  QILESLAWSHDSALWEALQVSANKVPTCEEVIFPNNFETGNGGNVQGHLPLMPMSPLMHPRVKEVRTDSG
  SLRRDMQPLSPISVHERYSSPTAGSAKRRLFGEDPPKEMLMDKIITEGTKLKIAPSSSITAENVSILPGQ
  TLLTMATAPVTGTTGHKVTIPLHGVANDAGEITLIPLSMNTNQESKVKSPVSLTAHSLIGASPKQTNLTK
  AQEVHSTGINRPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHL
  DQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPREVVAYNKNINDDFEMIDCDLE
  DATKTPDCSSGPVKEERGDLIKFYNTIYVGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRIS
  QQHSIYISPHKNGSGLTPRSALLYKFNGSPSKVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183404
• ORF Size: 3042 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183404.4
• RefSeq Size: 3305 bp
• RefSeq ORF: 3045 bp
• Locus ID: 5933
• UniProt ID: P28749
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• MW: 114.7 kDa
• Gene Summary: The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]