p107 (RBL1) (NM_183404) Human Untagged Clone

CAT#: SC109715

RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p107
• Synonyms: CP107; p107; PRB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_183404, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCGCTACAGGCCC
  TGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGACGACTTTACTGCCATCCGAGG
  CAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCATGTTCATTATATGTTGCATGCCGCAAAAGC
  ATTATTCCCACGGTTGGAAAGGGTATCATGGAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAG
  CTAAATTAAGTTTAATACAATTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGA
  ATTTCGTGAACGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
  CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGCCGGAAGCAGA
  GGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTTTTTGTTTATACTAAGGGTAA
  TTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCATTTACTTCTATGCTGCTTGGATCTGATTTTT
  GCCAATGCGATTATGTGCCCAAATAGACAAGACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATT
  TTCATACTGCTGACTTTACGGCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGA
  TGGACTTCTCGTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
  AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAAGCAGTGAATA
  AGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATCTTTTTGGGAGCAGACGCAGA
  AGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACCCCATTAGGGAAACTGACAGCACAGGCTAAT
  GTGGAGTATAACCTTCAACAGCACTTTGAAAAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGAC
  GGAGATATTTACGAGAAAAAGAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTT
  ACAGAGTATTGTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
  CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAACACTATACTC
  AATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGACTAAAGCTGGCAGAAATTTT
  GTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACACGAAGACTTCATGGAATGGACATGTCAGTT
  CTTTTAGAGCAAGATATATTTCATCGTTCCTTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATA
  GCTCACCTCGTACTTTTCCTTGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTAT
  TGAGGTGGTGATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
  CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTTTCTGCAAACA
  AAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGAAATGGAGGAAATGTGCAGGG
  ACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCAAGAGTCAAGGAAGTTCGAACTGACAGTGGG
  AGTCTTCGAAGAGATATGCAACCATTGTCTCCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAG
  GGAGTGCTAAGAGAAGACTCTTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGA
  AGGAACAAAATTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
  ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATTCCATTACATG
  GTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAATACAAATCAGGAGTCCAAAGT
  CAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGTGCTTCTCCAAAACAGACCAATCTGACTAAA
  GCACAAGAGGTACATTCAACTGGAATAAACAGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAA
  AGGTCTATCATTTGGCAAGTGTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACG
  AAGGAAGATATGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
  GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACTTTTCAAGAAA
  TTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGAAGTGTTCTGCTGAAAAGTAT
  TCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGATGACTTTGAAATGATAGATTGTGACTTAGAA
  GATGCTACAAAAACACCTGACTGTTCCAGTGGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTT
  ACAATACAATATATGTAGGAAGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATAT
  GATGGATGCTCCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
  CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGCGCTCTGCTGT
  ACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183404 unedited
  TAATACCGACTTCACCTATNAGTGGANCGGCACCGCGNAATTNCGGCCACGAAGGGCGCC
  CTGGGNAGAGGAGAAAGAAGTCGGGGGCCGTGGCGCGCAGCCCGCGGGGCCTGAAGGGAT
  GTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCGCT
  ACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGACGA
  CTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCATG
  TTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATGGA
  AGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAATT
  TTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAACG
  TATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAGCC
  AATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGCCG
  GAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTNTN
  TGTTTATACTAAGGGTAATTNTCGGATGATTGGGGATGACTTANTAAACTCTTATCATTT
  ACTTCTATGCTGCTAGGATCTGATNTTTGCCAATGCGATTATGTGCCCAAATANGACAGA
  CTTTGCTAACCATCATTTAAGGGTTACATCTGATNTTATACTGCTGACTTACGGCTNNCT
  GAGAGCACCTGCATCATTGCTGTACTGGGNGACTGCATGATGGACTNCTCGTAAAGCAAA
  GGGATAAGGAGCCCTCTCTAGCCCTTATTCAAACTCTTGCAGGAGATTAAAGGAAATGCC
  TCTGACCTTNCAGTTACGTATAGCAAC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_183404 unedited
  CTCCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTT
  CCCAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAA
  GCGCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTGAATAGGCTAAAAGT
  TGGATTTCAGATTAACAGTCAGTAACTGTTAATCCTGCCTCTTTTTTTTTTTTTCTTTTT
  TGGCCAAGTTACTACTATACTAATAAAATATGAAATGTTAAAAAAAAAAAAAAAAAACTC
  GACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCT
  GTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCC
  TTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTA
  TGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183404
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183404.1, NP_899662.1
• RefSeq Size: 3371 bp
• RefSeq ORF: 3045 bp
• Locus ID: 5933
• UniProt ID: P28749
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.