Centaurin gamma 1 (AGAP2) (NM_014770) Human Tagged ORF Clone

CAT#: RC206993

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AGAP2 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2



  "NM_014770" in other vectors (4)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Centaurin gamma 1 (AGAP2) (NM_014770) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AGAP2
Synonyms CENTG1; GGAP2; PIKE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC206993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATGCCCAGAGGCAGTTCGTTGTAGCTGCAGTGAGAGCAGAAGTCAGACGACATGAGGTGGCCAAGC
AGGCTCTAAACCGCCTCAGGAAGCTGGCAGAGAGGGTGGACGACCCCGAACTCCAGGACAGCATCCAGGC
CTCATTGGACAGCATTCGAGAGGCTGTGATCAATAGCCAGGAATGGACTTTGAGCCGCTCCATTCCTGAA
CTGCGCCTGGGTGTGCTGGGCGATGCCAGGAGTGGGAAGTCATCGCTCATCCACCGATTCCTGACTGGCT
CATACCAGGTGCTGGAGAAGACAGAGAGTGAGCAGTACAAGAAAGAAATGTTGGTGGATGGACAGACACA
TCTGGTGCTAATCCGAGAGGAAGCTGGGGCACCTGATGCCAAGTTCTCAGGCTGGGCAGATGCTGTGATC
TTCGTCTTCAGCCTGGAGGATGAGAACAGTTTCCAGGCTGTGAGCCGTCTCCATGGGCAGCTGAGTTCCC
TTCGCGGGGAGGGACGAGGAGGCCTGGCCTTGGCACTGGTGGGGACACAAGACAGGATCAGTGCTTCCTC
CCCTCGGGTGGTGGGAGATGCTCGTGCCAGAGCTCTGTGCGCGGACATGAAACGCTGCAGCTACTATGAG
ACTTGTGCAACCTATGGGCTCAATGTGGATCGGGTCTTCCAGGAGGTGGCCCAGAAGGTGGTGACCTTGC
GCAAGCAGCAACAGCTTCTGGCTGCCTGCAAGTCCCTTCCCAGCTCCCCAAGCCACTCAGCTGCATCCAC
TCCGGTAGCTGGCCAGGCTAGTAACGGGGGCCACACTAGCGACTACTCTTCTTCCCTCCCGTCCTCACCG
AATGTTGGTCACCGGGAGCTCCGAGCCGAGGCAGCTGCAGTGGCTGGATTGAGCACCCCAGGGTCCCTGC
ACCGGGCAGCCAAGCGCAGGACCAGCCTTTTTGCGAATCGTCGGGGTAGTGACTCCGAGAAACGAAGCTT
GGATAGTCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCTACTAAAACGA
AGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTAACCCTGTCCAGTAATGGCTTTCTACTCT
ACCACCCCAGTATTAACGATTACATCCACAGTACCCACGGCAAGGAGATGGACTTGCTGCGAACAACAGT
CAAAGTCCCGGGCAAGCGGCCCCCGAGGGCCATCTCTGCCTTTGGCCCCTCAGCCAGCATTAACGGGCTC
GTCAAGGACATGAGCACTGTCCAGATGGGTGAAGGCCTGGAAGCCACTACTCCCATGCCAAGCCCTAGCC
CCAGCCCCAGTTCCCTGCAGCCACCACCAGATCAGACATCCAAACACCTGCTGAAGCCAGACCGGAATTT
GGCCCGAGCCCTCAGCACGGACTGTACCCCATCTGGAGACCTGAGCCCCCTGAGTCGGGAACCCCCTCCT
TCTCCCATGGTGAAGAAGCAGAGGAGGAAAAAATTGACAACACCATCCAAGACTGAAGGCTCGGCTGGGC
AGGCTGAAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACGGGTCAGACGTGGCACTTTGAGGCAGC
CAGTTTTGAGGAGCGGGATGCCTGGGTCCAGGCCATCGAGAGTCAGATCCTAGCCAGTCTGCAATGCTGT
GAGAGCAGCAAGGTCAAGCTGCGCACAGACAGCCAAAGCGAGGCCGTGGCCATCCAGGCGATCCGGAACG
CCAAGGGGAATTCAATCTGCGTGGACTGCGGGGCCCCCAACCCCACGTGGGCCAGCTTGAACCTGGGCGC
CCTCATCTGCATCGAGTGTTCTGGCATCCACCGCAACCTGGGCACACACCTGTCCCGCGTTCGCTCGCTG
GACTTGGACGACTGGCCACGGGAGCTGACCCTGGTGCTGACGGCTATTGGCAACGACACGGCCAACCGCG
TGTGGGAAAGCGACACGCGAGGCCGTGCCAAGCCCTCGCGGGACTCTTCGCGGGAGGAGCGCGAGTCGTG
GATTCGCGCCAAGTACGAGCAGCTACTGTTCCTGGCGCCGCTGAGCACCTCGGAGGAGCCGCTGGGCCGC
CAGCTGTGGGCCGCCGTGCAGGCCCAGGACGTGGCTACCGTTCTCCTGCTTTTGGCCCATGCGCGACACG
GGCCGCTCGACACCAGCGTAGAGGACCCACAGCTGCGCTCCCCACTCCACCTGGCGGCCGAGCTCGCCCA
CGTCGTCATCACGCAACTGCTGCTGTGGTACGGCGCGGACGTGGCGGCCCGTGACGCCCAGGGCCGCACG
GCGCTGTTCTACGCCCGCCAGGCTGGAAGCCAGCTGTGCGCCGACATCCTTCTCCAGCACGGCTGCCCGG
GTGAGGGCGGCAGCGCGGCCACCACGCCCAGCGCGGCCACCACGCCCAGCATCACCGCCACGCCCAGCCC
CCGCCGCCGGAGCAGCGCCGCTAGCGTGGGCCGCGCCGACGCCCCGGTTGCGCTGGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206993 protein sequence
Red=Cloning site Green=Tags(s)

MHAQRQFVVAAVRAEVRRHEVAKQALNRLRKLAERVDDPELQDSIQASLDSIREAVINSQEWTLSRSIPE
LRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVI
FVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYE
TCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSP
NVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKR
SGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGL
VKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPP
SPMVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCC
ESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSL
DLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGR
QLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRT
ALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_014770
ORF Size 2508 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014770.1, NM_014770.2, NM_014770.3, NP_055585.1
RefSeq Size 4860 bp
RefSeq ORF 2511 bp
Locus ID 116986
Cytogenetics 12q14.1
Domains ArfGap, PH, ANK, RAS, RAB
Protein Pathways Endocytosis
MW 90.5 kDa
Gene Summary The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Citations (1)

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