PPP2R5B (NM_006244) Human Tagged ORF Clone

CAT#: RC206889

PPP2R5B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B)

Product Images

Circular map for RC206889

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  Western blot validation of overexpression lysate (Cat# LY401881) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206889 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPP2R5B protein (Cat# TP306889). The protein was produced from HEK293T cells transfected with PPP2R5B cDNA clone (Cat# RC206889) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R5B
• Synonyms: B56B; B56beta; PR61B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206889 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCGCCTGTGCCCC
  CACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGGCCCCGCCGCTCCCACAGCTC
  CTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACACCGCTGCCCCTGCTCAAAGATGTGCCGGCT
  TCCGAGCTGCACGAGCTGCTGAGCCGGAAGCTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTG
  TGGCCGACCTCAAGGGGAAGGAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCAC
  CCGGGGTGTCCTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACT
  CTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCTTCGTGGCCAC
  ACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGACTTCCAGCCCTCCGTGGCCAA
  GAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAGCTATTTGATAGTGAGGATCCCCGGGAGCGT
  GAGTACCTCAAGACCATCCTGCACCGGGTCTATGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAAC
  AGTGCAACCACATCTTCCTCCGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGA
  GATCCTAGGAAGCATCATCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGC
  GTCCTGATCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATACTGTGTGGTGC
  AGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTGCTCAAATACTGGCCAAAAAC
  CTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAAGAGATTCTTGATGTCATCGAGCCCTCCCAG
  TTTGTGAAGATCCAGGAGCCCCTTTTTAAGCAGGTGGCTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTG
  CAGAGCGGGCTCTGTATTTCTGGAACAATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGT
  GCTGCCTGCTGTGTTTGGGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTG
  ATCTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACAGCCTCCTACA
  AGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTATGGCAAGGTCTGGAGGAGCT
  GCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAGGCCCCCCTCCAGCGGCTTACACCCCAGGTG
  GCCGCCAGTGGGGGTCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206889 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELTPLPLLKDVPA
  SELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRT
  LPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPRER
  EYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVR
  VLIPLHSVKSLSVFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQ
  FVKIQEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSL
  IYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQV
  AASGGQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006244
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006244.4
• RefSeq Size: 2766 bp
• RefSeq ORF: 1494 bp
• Locus ID: 5526
• UniProt ID: Q15173
• Cytogenetics: 11q13.1
• Domains: B56
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• MW: 57.4 kDa
• Gene Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq, Jul 2008]