PLA2G3 (NM_015715) Human Tagged ORF Clone

CAT#: RC206872

PLA2G3 (Myc-DDK-tagged)-Human phospholipase A2, group III (PLA2G3)

Product Images

Circular map for RC206872

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PLA2G3 (Cat# RC206872, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLA2G3 (Cat# TA811660)(1:2000).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLA2G3
• Synonyms: GIII-SPLA2; sPLA2-III; SPLA2III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206872 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTTCAGGCAGGGCTGTTTGGGATGCTGGGCTTCCTGGGGGTGGCCCTGGGGGGCTCCCCTGCCC
  TCCGCTGGTACAGGACCTCCTGCCACTTGACCAAGGCCGTCCCTGGCAACCCACTGGGGTACCTGAGCTT
  CCTGGCCAAGGATGCTCAGGGACTGGCCCTGATCCATGCCCGCTGGGATGCGCATAGGAGGCTGCAGGCA
  TGTAGCTGGGAGGATGAGCCGGAGCTCACCGCAGCCTACGGTGCTCTCTGTGCTCATGAGACTGCCTGGG
  GCTCCTTCATCCACACCCCCGGACCCGAGCTGCAGAGAGCACTGGCCACTCTTCAGAGTCAGTGGGAGGC
  ATGCCGAGCGCTTGAGGAGAGTCCAGCAGGGGCCAGGAAGAAGCGAGCAGCAGGGCAGAGTGGAGTCCCT
  GGTGGAGGGCACCAGCGAGAGAAGAGAGGATGGACCATGCCTGGCACACTGTGGTGTGGAGTTGGAGATT
  CTGCTGGGAACTCCTCGGAGCTGGGGGTCTTCCAGGGACCTGATCTCTGTTGCCGGGAACATGACCGCTG
  CCCACAGAACATCTCACCCTTGCAGTACAACTATGGCATCCGAAACTACCGATTCCACACCATCTCCCAC
  TGTGACTGTGACACCAGGTTTCAGCAATGCCTACAGAATCAGCACGACTCCATCTCGGACATCGTGGGCG
  TGGCCTTCTTCAACGTGCTGGAGATCCCCTGCTTTGTGCTGGAGGAGCAGGAGGCGTGTGTGGCGTGGTA
  CTGGTGGGGCGGGTGTAGGATGTATGGCACAGTGCCCCTCGCTCGCCTGCAGCCCAGGACCTTCTACAAT
  GCCTCCTGGAGCTCCCGGGCCACCTCCCCAACTCCCAGCTCCCGGAGCCCAGCCCCTCCCAAGCCTCGAC
  AGAAGCAGCACCTTCGGAAGGGGCCACCACATCAGAAAGGGTCCAAGCGCCCCAGCAAAGCCAACACCAC
  AGCCCTCCAGGACCCTATGGTCTCTCCCAGGCTTGATGTGGCCCCCACAGGCCTCCAGGGCCCACAGGGT
  GGCCTAAAACCTCAGGGTGCCCGCTGGGTCTGCCGCAGCTTCCGCCGCCACCTGGACCAGTGTGAGCACC
  AGATTGGGCCCCGGGAAATCGAGTTCCAGCTGCTCAACAGCGCCCAAGAGCCCCTCTTCCACTGCAACTG
  CACGCGCCGTCTGGCACGCTTCCTGAGGCTCCACAGCCCACCCGAGGTTACCAACATGCTTTGGGAGCTG
  CTGGGCACAACCTGCTTCAAGCTGGCCCCTCCACTGGACTGTGTGGAAGGCAAAAACTGTTCCAGAGACC
  CTAGGGCCATCAGGGTGTCAGCCCGGCACTTGCGGAGGCTTCAGCAGAGGCGACACCAGCTCCAGGATAA
  AGGCACAGATGAGAGGCAGCCATGGCCTTCAGAGCCCCTGAGAGGCCCCATGTCATTCTACAACCAGTGC
  CTGCAGCTAACCCAGGCAGCCAGGAGACCCGACAGGCAGCAGAAGTCCTGGAGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206872 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVQAGLFGMLGFLGVALGGSPALRWYRTSCHLTKAVPGNPLGYLSFLAKDAQGLALIHARWDAHRRLQA
  CSWEDEPELTAAYGALCAHETAWGSFIHTPGPELQRALATLQSQWEACRALEESPAGARKKRAAGQSGVP
  GGGHQREKRGWTMPGTLWCGVGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISH
  CDCDTRFQQCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPRTFYN
  ASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMVSPRLDVAPTGLQGPQG
  GLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPLFHCNCTRRLARFLRLHSPPEVTNMLWEL
  LGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQC
  LQLTQAARRPDRQQKSWSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015715
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015715.5
• RefSeq Size: 2717 bp
• RefSeq ORF: 1530 bp
• Locus ID: 50487
• UniProt ID: Q9NZ20
• Cytogenetics: 22q12.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 57.2 kDa
• Gene Summary: This gene encodes a protein that belongs to the secreted phospholipase A2 family, whose members include the bee venom enzyme. The encoded enzyme functions in lipid metabolism and catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of phospholipids to release arachidonic acid and lysophospholipids. This enzyme acts as a negative regulator of ciliogenesis, and may play a role in cancer development by stimulating tumor cell growth and angiogenesis. This gene is associated with oxidative stress, and polymorphisms in this gene are linked to risk for Alzheimer's disease. [provided by RefSeq, Apr 2014]