PLD3 (NM_001031696) Human Tagged ORF Clone

CAT#: RC206783

PLD3 (Myc-DDK-tagged)-Human phospholipase D family, member 3 (PLD3), transcript variant 1

Product Images

Circular map for RC206783

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  Western blot validation of overexpression lysate (Cat# LY415872) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218761 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PLD3 protein (Cat# TP306783). The protein was produced from HEK293T cells transfected with PLD3 cDNA clone (Cat# RC206783) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLD3
• Synonyms: AD19; HU-K4; HUK4; SCA46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206783 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCTAAACTGATGTACCAGGAGCTGAAGGTGCCTGCAGAGGAGCCCGCCAATGAGCTGCCCATGA
  ATGAGATTGAGGCGTGGAAGGCTGCGGAAAAGAAAGCCCGCTGGGTCCTGCTGGTCCTCATTCTGGCGGT
  TGTGGGCTTCGGAGCCCTGATGACTCAGCTGTTTCTATGGGAATACGGCGACTTGCATCTCTTTGGGCCC
  AACCAGCGCCCAGCCCCCTGCTATGACCCTTGCGAAGCAGTGCTGGTGGAAAGCATTCCTGAGGGCCTGG
  ACTTCCCCAATGCCTCCACGGGGAACCCTTCCACCAGCCAGGCCTGGCTGGGCCTGCTCGCCGGTGCGCA
  CAGCAGCCTGGACATCGCCTCCTTCTACTGGACCCTCACCAACAATGACACCCACACGCAGGAGCCCTCT
  GCCCAGCAGGGTGAGGAGGTCCTCCGGCAGCTGCAGACCCTGGCACCAAAGGGCGTGAACGTCCGCATCG
  CTGTGAGCAAGCCCAGCGGGCCCCAGCCACAGGCGGACCTGCAGGCTCTGCTGCAGAGCGGTGCCCAGGT
  CCGCATGGTGGACATGCAGAAGCTGACCCATGGCGTCCTGCATACCAAGTTCTGGGTGGTGGACCAGACC
  CACTTCTACCTGGGCAGTGCCAACATGGACTGGCGTTCACTGACCCAGGTCAAGGAGCTGGGCGTGGTCA
  TGTACAACTGCAGCTGCCTGGCTCGAGACCTGACCAAGATCTTTGAGGCCTACTGGTTCCTGGGCCAGGC
  AGGCAGCTCCATCCCATCAACTTGGCCCCGGTTCTATGACACCCGCTACAACCAAGAGACACCAATGGAG
  ATCTGCCTCAATGGAACCCCTGCTCTGGCCTACCTGGCGAGTGCGCCCCCACCCCTGTGTCCAAGTGGCC
  GCACTCCAGACCTGAAGGCTCTACTCAACGTGGTGGACAATGCCCGGAGTTTCATCTACGTCGCTGTCAT
  GAACTACCTGCCCACTCTGGAGTTCTCCCACCCTCACAGGTTCTGGCCTGCCATTGACGATGGGCTGCGG
  CGGGCCACCTACGAGCGTGGCGTCAAGGTGCGCCTGCTCATCAGCTGCTGGGGACACTCGGAGCCATCCA
  TGCGGGCCTTCCTGCTCTCTCTGGCTGCCCTGCGTGACAACCATACCCACTCTGACATCCAGGTGAAACT
  CTTTGTGGTCCCCGCGGATGAGGCCCAGGCTCGAATCCCATATGCCCGTGTCAACCACAACAAGTACATG
  GTGACTGAACGCGCCACCTACATCGGAACCTCCAACTGGTCTGGCAACTACTTCACGGAGACGGCGGGCA
  CCTCGCTGCTGGTGACGCAGAATGGGAGGGGCGGCCTGCGGAGCCAGCTGGAGGCCATTTTCCTGAGGGA
  CTGGGACTCCCCTTACAGCCATGACCTTGACACCTCAGCTGACAGCGTGGGCAACGCCTGCCGCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206783 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKPKLMYQELKVPAEEPANELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGP
  NQRPAPCYDPCEAVLVESIPEGLDFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEPS
  AQQGEEVLRQLQTLAPKGVNVRIAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQT
  HFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPME
  ICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLR
  RATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYM
  VTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031696
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031696.3
• RefSeq Size: 2207 bp
• RefSeq ORF: 1473 bp
• Locus ID: 23646
• UniProt ID: Q8IV08
• Cytogenetics: 19q13.2
• Protein Families: Transmembrane
• MW: 54.7 kDa
• Gene Summary: This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2014]