SLC39A5 (NM_173596) Human Tagged ORF Clone

CAT#: RC206665

SLC39A5 (Myc-DDK-tagged)-Human solute carrier family 39 (metal ion transporter), member 5 (SLC39A5), transcript variant 1

Product Images

Circular map for RC206665

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC39A5 (Cat# RC206665, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC39A5 (Cat# TA810144)(1:2000). Positive lysates LY406498 (100ug) and LC406498 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY406498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLC39A5 protein (Cat# TP306665). The protein was produced from HEK293T cells transfected with SLC39A5 cDNA clone (Cat# RC206665) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC39A5
• Synonyms: LZT-Hs7; MYP24; ZIP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206665 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCCCCAGTGAGTCATCTGCTGGCCGGCTTCTGTGTGTGGGTCGTCTTGGGCTGGGTAGGGGGCT
  CAGTCCCCAACCTGGGCCCTGCTGAGCAGGAGCAGAACCATTACCTGGCCCAGCTGTTTGGCCTGTACGG
  CGAGAATGGGACGCTGACTGCAGGGGGCTTGGCGCGGCTTCTCCACAGCCTGGGGCTAGGCCGAGTTCAG
  GGGCTTCGCCTGGGACAGCATGGGCCTCTGACTGGACGGGCTGCATCCCCAGCTGCAGACAATTCCACAC
  ACAGGCCACAGAACCCTGAGCTGAGTGTGGATGTCTGGGCAGGGATGCCTCTGGGTCCCTCAGGGTGGGG
  TGACCTGGAAGAGTCAAAGGCCCCTCACCTACCCCGTGGGCCAGCCCCCTCGGGCCTGGACCTCCTTCAC
  AGGCTTCTGTTGCTGGACCACTCATTGGCTGACCACCTGAATGAGGATTGTCTGAACGGCTCCCAGCTGC
  TGGTCAATTTTGGCTTGAGCCCCGCTGCTCCTCTGACCCCTCGTCAGTTTGCTCTGCTGTGCCCAGCCCT
  GCTTTATCAGATCGACAGCCGCGTCTGCATCGGCGCTCCGGCCCCTGCACCCCCAGGGGATCTACTATCT
  GCCCTGCTTCAGAGTGCCCTGGCAGTCCTGTTGCTCAGCCTCCCTTCTCCCCTATCCCTGCTGCTGCTGC
  GGCTCCTGGGACCTCGTCTACTACGGCCCTTGCTGGGCTTCCTGGGGGCCCTGGCGGTGGGCACTCTTTG
  TGGGGATGCACTGCTACATCTGCTACCGCATGCACAAGAAGGGCGGCACGCAGGACCTGGCGGACTACCA
  GAGAAGGACCTGGGCCCGGGGCTGTCAGTGCTCGGAGGCCTCTTCCTGCTCTTTGTGCTGGAGAACATGC
  TGGGGCTTTTGCGGCACCGAGGGCTCAGGCCAAGATGCTGCAGGCGAAAACGAAGGAATCTCGAAACACG
  CAACTTGGATCCGGAGAATGGCAGTGGGATGGCCCTTCAGCCCCTACAGGCAGCTCCAGAGCCAGGGGCT
  CAGGGCCAGAGGGAGAAGAACAGCCAGCACCCACCAGCTCTGGCCCCTCCTGGGCACCAAGGCCACAGTC
  ATGGGCACCAGGGTGGCACTGATATCACGTGGATGGTCCTCCTGGGAGATGGTCTACACAACCTCACTGA
  TGGGCTGGCCATAGGTGCTGCCTTCTCTGATGGCTTCTCCAGCGGCCTCAGTACCACCTTAGCGGTCTTC
  TGCCATGAGCTGCCCCACGAACTGGGTGACTTTGCCATGCTGCTCCAGTCAGGGCTGTCCTTTCGGCGGC
  TGCTGCTGCTGAGCCTCGTGTCTGGAGCCCTGGGATTGGGGGGTGCAGTCCTGGGGGTGGGGCTCAGCCT
  GGGCCCTGTCCCCCTCACTCCCTGGGTGTTTGGGGTCACTGCTGGGGTCTTCCTCTATGTGGCCCTTGTG
  GACATGCTACCAGCCCTGCTTCGTCCTCCGGAGCCCCTGCCTACGCCCCATGTGCTCCTGCAGGGGCTGG
  GGCTGCTGCTGGGGGGCGGCCTCATGCTTGCCATAACCCTGCTGGAGGAGCGGCTACTGCCCGTGACCAC
  TGAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206665 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSPVSHLLAGFCVWVVLGWVGGSVPNLGPAEQEQNHYLAQLFGLYGENGTLTAGGLARLLHSLGLGRVQ
  GLRLGQHGPLTGRAASPAADNSTHRPQNPELSVDVWAGMPLGPSGWGDLEESKAPHLPRGPAPSGLDLLH
  RLLLLDHSLADHLNEDCLNGSQLLVNFGLSPAAPLTPRQFALLCPALLYQIDSRVCIGAPAPAPPGDLLS
  ALLQSALAVLLLSLPSPLSLLLLRLLGPRLLRPLLGFLGALAVGTLCGDALLHLLPHAQEGRHAGPGGLP
  EKDLGPGLSVLGGLFLLFVLENMLGLLRHRGLRPRCCRRKRRNLETRNLDPENGSGMALQPLQAAPEPGA
  QGQREKNSQHPPALAPPGHQGHSHGHQGGTDITWMVLLGDGLHNLTDGLAIGAAFSDGFSSGLSTTLAVF
  CHELPHELGDFAMLLQSGLSFRRLLLLSLVSGALGLGGAVLGVGLSLGPVPLTPWVFGVTAGVFLYVALV
  DMLPALLRPPEPLPTPHVLLQGLGLLLGGGLMLAITLLEERLLPVTTEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173596
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173596.1, NP_775867.1
• RefSeq Size: 2015 bp
• RefSeq ORF: 1623 bp
• Locus ID: 283375
• UniProt ID: Q6ZMH5
• Cytogenetics: 12q13.3
• Protein Families: Transmembrane
• MW: 56.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the ZIP family of zinc transporters that transport zinc into cells from outside, and play a crucial role in controlling intracellular zinc levels. Zinc is an essential cofactor for many enzymes and proteins involved in gene transcription, growth, development and differentiation. Mutations in this gene have been associated with autosomal dominant high myopia (MYP24). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]