REG1 beta (REG1B) (NM_006507) Human Tagged ORF Clone

CAT#: RC206451

REG1B (Myc-DDK-tagged)-Human regenerating islet-derived 1 beta (REG1B)

Product Images

Circular map for RC206451

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  Western blot validation of overexpression lysate (Cat# LY416596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206451 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified REG1B protein (Cat# TP306451). The protein was produced from HEK293T cells transfected with REG1B cDNA clone (Cat# RC206451) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REG1 beta
• Synonyms: PSPS2; REGH; REGI-BETA; REGL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206451 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGACCAACTCGTTCTTCATGCTGATCTCCTCCCTGATGTTCCTGTCTCTGAGCCAAGGCCAGG
  AGTCCCAGACAGAGCTGCCTAATCCCCGAATCAGCTGCCCAGAAGGCACCAATGCCTATCGCTCCTACTG
  CTACTACTTTAATGAAGACCCTGAGACCTGGGTTGATGCAGATCTCTATTGCCAGAACATGAATTCAGGC
  AACCTGGTGTCTGTGCTCACCCAGGCGGAGGGTGCCTTCGTGGCCTCACTGATTAAGGAGAGTAGCACTG
  ATGACAGCAATGTCTGGATTGGCCTCCATGACCCAAAAAAGAACCGCCGCTGGCACTGGAGTAGTGGGTC
  CCTGGTCTCCTACAAGTCCTGGGACACTGGATCCCCGAGCAGTGCTAATGCTGGCTACTGTGCAAGCCTG
  ACTTCATGCTCAGGATTCAAGAAATGGAAGGATGAATCTTGTGAGAAGAAGTTCTCCTTTGTTTGCAAGT
  TCAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206451 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQTNSFFMLISSLMFLSLSQGQESQTELPNPRISCPEGTNAYRSYCYYFNEDPETWVDADLYCQNMNSG
  NLVSVLTQAEGAFVASLIKESSTDDSNVWIGLHDPKKNRRWHWSSGSLVSYKSWDTGSPSSANAGYCASL
  TSCSGFKKWKDESCEKKFSFVCKFKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006507
• ORF Size: 498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006507.4
• RefSeq Size: 812 bp
• RefSeq ORF: 501 bp
• Locus ID: 5968
• UniProt ID: P48304
• Cytogenetics: 2p12
• MW: 18.7 kDa
• Gene Summary: This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins. This gene encodes a protein secreted by the exocrine pancreas that is highly similar to the REG1A protein. The related REG1A protein is associated with islet cell regeneration and diabetogenesis, and may be involved in pancreatic lithogenesis. Reg family members REG1A, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]