EIF5 (NM_001969) Human Tagged ORF Clone

CAT#: RC206301

EIF5 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 1

Product Images

Circular map for RC206301

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  Western blot validation of overexpression lysate (Cat# LY405302) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EIF5 protein (Cat# TP306301). The protein was produced from HEK293T cells transfected with EIF5 cDNA clone (Cat# RC206301) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF5
• Synonyms: EIF-5; EIF-5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206301 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCA
  AGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA
  TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG
  AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA
  AAAAATTTGTTCTCTGTCCTGAATGTGAGAATCCTGAAACAGATTTGCATGTCAATCCAAAGAAGCAAAC
  AATAGGTAATTCTTGTAAAGCCTGTGGCTATCGAGGCATGCTTGACACACATCATAAACTCTGCACATTC
  ATTCTCAAAAACCCACCTGAGAATAGTGACAGTGGTACAGGAAAGAAAGAAAAAGAAAAGAAAAACAGAA
  AGGGCAAAGACAAGGAAAATGGCTCCGTATCCAGCAGTGAGACACCACCACCACCACCACCACCAAATGA
  AATTAATCCTCCTCCACATACAATGGAAGAAGAGGAGGATGATGACTGGGGAGAAGATACAACTGAGGAA
  GCTCAAAGGCGTCGAATGGATGAAATCAGTGACCATGCAAAAGTTCTGACACTCAGTGATGATTTGGAAA
  GAACAATTGAGGAGAGGGTCAATATCCTCTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGTGTTATTGA
  TTCATCTGACAAAGAAATCGTTGCTGAAGCAGAAAGACTGGATGTAAAAGCCATGGGCCCTCTTGTTCTA
  ACTGAAGTTCTTTTTAATGAGAAGATTAGAGAACAGATTAAGAAATACAGGCGCCATTTCCTACGATTTT
  GTCACAACAACAAAAAAGCCAAACGGTACCTTCTTCATGGTTTGGAGTGTGTGGTAGCAATGCATCAAGC
  TCAGCTTATCTCCAAGATTCCACATATCTTGAAGGAGATGTACGATGCAGACCTTTTAGAAGAAGAGGTC
  ATCATCAGCTGGTCGGAAAAGGCCTCTAAGAAATATGTCTCCAAAGAACTTGCCAAAGAGATTCGTGTCA
  AAGCAGAACCATTTATAAAATGGTTGAAGGAGGCAGAGGAAGAATCTTCTGGTGGCGAAGAAGAAGATGA
  AGATGAGAACATTGAGGTGGTGTATTCGAAGGCTGCCAGTGTACCGAAAGTTGAGACTGTAAAGTCAGAC
  AACAAGGATGACGACATCGATATTGATGCCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206301 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK
  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTF
  ILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEE
  AQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVL
  TEVLFNEKIREQIKKYRRHFLRFCHNNKKAKRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEV
  IISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSD
  NKDDDIDIDAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001969
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001969.5
• RefSeq Size: 5963 bp
• RefSeq ORF: 1296 bp
• Locus ID: 1983
• UniProt ID: P55010
• Cytogenetics: 14q32.32
• Domains: eIF2B_5, eIF5C
• MW: 49.2 kDa
• Gene Summary: Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]