JPH3 (NM_020655) Human Tagged ORF Clone

CAT#: RC206252

JPH3 (Myc-DDK-tagged)-Human junctophilin 3 (JPH3)

Product Images

Circular map for RC206252

• 

  Western blot validation of overexpression lysate (Cat# LY402798) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206252 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JPH3
• Synonyms: CAGL237; HDL2; JP-3; JP3; TNRC22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206252 representing NM_020655
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGTGGGGGCAGGTTTAATTTTGACGACGGAGGGTCCTACTGTGGAGGCTGGGAGGACGGCAAGG
  CGCACGGCCATGGCGTCTGCACCGGCCCCAAGGGCCAAGGCGAATACACCGGCTCGTGGAGCCACGGCTT
  CGAGGTGCTGGGCGTCTACACCTGGCCCAGCGGCAACACGTACCAGGGCACCTGGGCGCAGGGCAAGCGC
  CACGGCATCGGCCTGGAGAGCAAGGGGAAGTGGGTGTACAAGGGCGAGTGGACGCACGGATTCAAGGGGC
  GCTACGGGGTGCGGGAGTGCGCGGGCAACGGGGCCAAATACGAAGGGACCTGGAGCAACGGGCTGCAGGA
  CGGCTACGGGACCGAGACCTACTCGGACGGAGGGACCTACCAGGGCCAGTGGGTCGGTGGCATGCGCCAG
  GGCTACGGCGTCCGGCAGAGCGTCCCGTATGGCATGGCCGCGGTCATCCGCTCACCCCTGAGGACGTCCA
  TCAACTCCCTGCGCAGCGAGCACACCAACGGCACGGCGCTGCATCCCGACGCCTCTCCGGCGGTGGCCGG
  CAGCCCGGCCGTGTCCCGCGGGGGCTTCGTGCTCGTGGCCCACAGTGACTCCGAGATCCTCAAGAGCAAG
  AAGAAGGGGCTGTTTCGGCGCTCGCTGCTGAGTGGGCTGAAGCTGCGCAAGTCGGAGTCCAAGAGCAGCC
  TGGCCAGCCAACGCAGCAAGCAGAGCTCCTTTCGCAGCGAGGCGGGCATGAGCACCGTCAGCTCCACGGC
  CAGCGACATCCACTCCACCATCAGCCTGGGCGAGGCTGAGGCCGAGCTGGCGGTCATCGAGGACGACATC
  GACGCCACCACCACCGAGACCTACGTGGGCGAGTGGAAGAACGACAAACGCTCCGGCTTCGGCGTGAGCC
  AGCGCTCGGACGGGCTCAAGTACGAGGGCGAGTGGGCCAGCAACCGGCGCCATGGCTACGGCTGCATGAC
  CTTCCCGGACGGCACCAAGGAGGAGGGCAAGTACAAGCAGAACATCCTCGTCGGCGGCAAGCGCAAGAAC
  CTCATCCCCCTGCGGGCCAGCAAGATCCGCGAGAAGGTGGACCGCGCCGTTGAGGCCGCTGAGCGGGCCG
  CCACCACCGCCAAGCAGAAGGCTGAGATCGCGGCTTCCAGGACCTCCCACTCTCGGGCAAAGGCCGAGGC
  AGCCCTCACAGCAGCTCAGAAAGCCCAGGAGGAGGCGCGGATCGCCAGGATCACTGCCAAAGAGTTCTCC
  CCTTCCTTCCAGCACCGGGAAAACGGGCTGGAGTACCAGAGGCCGAAGCGTCAGACCTCCTGTGACGACA
  TCGAGGTGCTGTCCACCGGGACACCCCTGCAGCAGGAGAGCCCCGAGCTGTACCGCAAGGGCACCACTCC
  CTCCGACCTGACCACCGACGACAGCCCCCTGCAGAGCTTCCCCACCAGCCCCGCGGCCACCCCGCCGCCC
  GCGCCCGCCGCCAGGAACAAGGTCGCCCACTTCTCGAGGCAGGTGTCGGTGGACGAGGAGCGGGGCGGGG
  ACATCCAGATGCTCCTGGAGGGCCGGGCCGGGGACTGCGCCCGCAGCAGCTGGGGCGAGGAGCAGGCCGG
  GGGCTCCAGGGGTGTCCGCAGCGGTGCCCTGCGCGGCGGCCTGCTCGTGGATGACTTCCGCACCCGAGGT
  TCGGGCCGCAAGCAGCCCGGGAACCCCAAGCCGCGGGAGCGGCGGACGGAGTCACCCCCCGTGTTCACGT
  GGACTTCCCACCACCGGGCCAGCAACCACAGCCCCGGAGGCTCCAGGCTGCTGGAGCTGCAGGAGGAGAA
  GCTGAGCAACTACCGGATGGAGATGAAACCCTTGCTGAGGATGGAGACGCATCCCCAGAAAAGACGCTAC
  AGCAAGGGCGGCGCCTGCCGGGGCTTGGGGGACGACCACCGCCTCGAGGACCGGGGCTTCGGGGTGCAGA
  GACTGCGGTCCAAGGCCCAGAACAAGGAGAACTTCAGGCCGGCCTCCTCCGCGGAGCCCGCCGTGCAGAA
  ACTGGCGAGCCTGCGGCTGGGCGGGGCCGAGCCCCGGTTGCTGCGTTGGGACTTGACCTTCTCCCCGCCC
  CAGAAATCCTTGCCTGTCGCTCTAGAGTCCGACGAGGAGAATGGGGATGAGCTCAAGTCCAGTACGGGCT
  CAGCGCCTATCCTGGTGGTCATGGTGATCTTGCTCAACATCGGAGTCGCCATTCTGTTTATTAACTTTTT
  CATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206252 representing NM_020655
  Red=Cloning site Green=Tags(s)
  
  MSSGGRFNFDDGGSYCGGWEDGKAHGHGVCTGPKGQGEYTGSWSHGFEVLGVYTWPSGNTYQGTWAQGKR
  HGIGLESKGKWVYKGEWTHGFKGRYGVRECAGNGAKYEGTWSNGLQDGYGTETYSDGGTYQGQWVGGMRQ
  GYGVRQSVPYGMAAVIRSPLRTSINSLRSEHTNGTALHPDASPAVAGSPAVSRGGFVLVAHSDSEILKSK
  KKGLFRRSLLSGLKLRKSESKSSLASQRSKQSSFRSEAGMSTVSSTASDIHSTISLGEAEAELAVIEDDI
  DATTTETYVGEWKNDKRSGFGVSQRSDGLKYEGEWASNRRHGYGCMTFPDGTKEEGKYKQNILVGGKRKN
  LIPLRASKIREKVDRAVEAAERAATTAKQKAEIAASRTSHSRAKAEAALTAAQKAQEEARIARITAKEFS
  PSFQHRENGLEYQRPKRQTSCDDIEVLSTGTPLQQESPELYRKGTTPSDLTTDDSPLQSFPTSPAATPPP
  APAARNKVAHFSRQVSVDEERGGDIQMLLEGRAGDCARSSWGEEQAGGSRGVRSGALRGGLLVDDFRTRG
  SGRKQPGNPKPRERRTESPPVFTWTSHHRASNHSPGGSRLLELQEEKLSNYRMEMKPLLRMETHPQKRRY
  SKGGACRGLGDDHRLEDRGFGVQRLRSKAQNKENFRPASSAEPAVQKLASLRLGGAEPRLLRWDLTFSPP
  QKSLPVALESDEENGDELKSSTGSAPILVVMVILLNIGVAILFINFFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020655
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020655.4
• RefSeq Size: 3997 bp
• RefSeq ORF: 2247 bp
• Locus ID: 57338
• UniProt ID: Q8WXH2
• Cytogenetics: 16q24.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 81.3 kDa
• Gene Summary: Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. CAG/CTG repeat expansion from normally 6-28 repeats to 40-59 repeats in the 3' UTR of this gene have been associated with Huntington disease-like 2 (HDL2). This gene is a member of the junctophilin gene family. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2016]