ELL2 (NM_012081) Human Tagged ORF Clone

CAT#: RC206204

ELL2 (Myc-DDK-tagged)-Human elongation factor, RNA polymerase II, 2 (ELL2)

Product Images

Circular map for RC206204

• 

  Western blot validation of overexpression lysate (Cat# LY402140) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206204 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELL2
• Synonyms: MRCCAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206204 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGGGGGACAGGGGGCCTGCGGGAGGAGCAGCGCTATGGGCTGTCGTGCGGACGGCTGGGGC
  AGGACAACATCACCGTACTGCATGTGAAGCTCACCGAGACGGCGATCCGGGCGCTCGAGACTTACCAGAG
  CCACAAGAATTTAATTCCTTTTCGACCTTCAATCCAGTTCCAAGGACTCCACGGGCTTGTCAAAATTCCC
  AAAAATGATCCCCTCAATGAAGTTCATAACTTTAACTTTTATTTGTCAAATGTGGGCAAAGACAACCCTC
  AGGGCAGCTTTGACTGCATCCAGCAAACATTCTCCAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATT
  TATACAAGATAAAATTACAGTGTGTGCAACAAACGACTCGTATCAGATGACACGAGAAAGAATGACCCAG
  GCAGAGGAGGAATCCCGCAACCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTAGGGAAAAGAG
  TGCAAATTCGGAAAGCACCTCAAGCTGTTTCAGATACAGTTCCTGAGAGGAAAAGGTCAACCCCCATGAA
  CCCTGCAAATACAATTCGAAAGACACATAGCAGCAGCACCATCTCTCAGAGGCCATACAGGGACAGGGTG
  ATTCACTTACTGGCCCTGAAGGCCTACAAGAAACCGGAGCTACTTGCTAGACTCCAGAAAGATGGTGTCA
  ATCAAAAAGACAAGAACTCCCTGGGAGCAATTCTGCAACAGGTAGCCAATCTGAATTCTAAGGACCTCTC
  ATATACCTTAAAGGATTATGTTTTTAAAGAGCTTCAAAGAGACTGGCCTGGATACAGTGAAATAGACAGA
  CGGTCATTGGAGTCAGTGCTCTCTAGAAAACTAAATCCGTCTCAGAATGCTACAGGCACCAGCCGTTCAG
  AATCTCCTGTATGTTCTAGTAGAGATGCTGTATCTTCTCCTCAGAAACGGCTTTTGGATTCAGAGTTTAT
  TGATCCTTTAATGAATAAAAAAGCCCGAATATCTCACCTGACGAACAGAGTACCACCAACACTAAATGGT
  CATTTGAATCCCACCAGTGAAAAATCGGCTGCAGGCCTCCCACTGCCCCCTGCGGCTGCTGCCATCCCCA
  CCCCTCCACCGCTGCCTTCAACCTATCTGCCCATCTCACATCCTCCTCAGATTGTAAATTCTAACTCCAA
  CTCCCCTAGCACTCCAGAAGGCCGGGGGACTCAAGACCTACCTGTTGACAGTTTTAGTCAAAACGATAGT
  ATCTATGAGGACCAGCAAGACAAATATACCTCTAGGACTTCTCTGGAAACCTTACCCCCTGGTTCCGTTC
  TACTAAAGTGTCCAAAGCCTATGGAAGAAAACCATTCAATGTCTCACAAAAAGTCCAAAAAGAAGTCTAA
  AAAACATAAGGAAAAGGACCAAATAAAAAAGCACGACATTGAGACTATTGAGGAAAAGGAGGAAGATCTT
  AAGAGAGAAGAGGAAATTGCCAAGCTAAATAACTCCAGTCCAAATTCCAGTGGAGGAGTTAAAGAGGATT
  GCACTGCCTCCATGGAACCTTCAGCAATTGAACTCCCAGATTATTTGATAAAATATATCGCTATCGTCTC
  CTATGAGCAACGCCAGAATTATAAGGATGACTTCAATGCAGAGTATGATGAGTACAGAGCTTTGCATGCC
  AGGATGGAGACTGTAGCTAGAAGATTTATCAAACTAGATGCACAAAGAAAGCGCCTTTCTCCAGGCTCAA
  AAGAGTATCAGAATGTTCATGAAGAAGTCTTACAAGAATATCAGAAGATAAAGCAGTCTAGTCCCAATTA
  CCATGAAGAAAAATACAGATGTGAATATCTTCATAACAAGCTGGCTCACATCAAAAGGCTAATAGGTGAA
  TTTGACCAACAGCAAGCAGAGTCATGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206204 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGGTGGLREEQRYGLSCGRLGQDNITVLHVKLTETAIRALETYQSHKNLIPFRPSIQFQGLHGLVKIP
  KNDPLNEVHNFNFYLSNVGKDNPQGSFDCIQQTFSSSGASQLNCLGFIQDKITVCATNDSYQMTRERMTQ
  AEEESRNRSTKVIKPGGPYVGKRVQIRKAPQAVSDTVPERKRSTPMNPANTIRKTHSSSTISQRPYRDRV
  IHLLALKAYKKPELLARLQKDGVNQKDKNSLGAILQQVANLNSKDLSYTLKDYVFKELQRDWPGYSEIDR
  RSLESVLSRKLNPSQNATGTSRSESPVCSSRDAVSSPQKRLLDSEFIDPLMNKKARISHLTNRVPPTLNG
  HLNPTSEKSAAGLPLPPAAAAIPTPPPLPSTYLPISHPPQIVNSNSNSPSTPEGRGTQDLPVDSFSQNDS
  IYEDQQDKYTSRTSLETLPPGSVLLKCPKPMEENHSMSHKKSKKKSKKHKEKDQIKKHDIETIEEKEEDL
  KREEEIAKLNNSSPNSSGGVKEDCTASMEPSAIELPDYLIKYIAIVSYEQRQNYKDDFNAEYDEYRALHA
  RMETVARRFIKLDAQRKRLSPGSKEYQNVHEEVLQEYQKIKQSSPNYHEEKYRCEYLHNKLAHIKRLIGE
  FDQQQAESWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012081
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012081.4
• RefSeq Size: 6063 bp
• RefSeq ORF: 1923 bp
• Locus ID: 22936
• UniProt ID: O00472
• Cytogenetics: 5q15
• Protein Families: Transcription Factors
• MW: 72.4 kDa
• Gene Summary: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968). Plays a role in immunoglobulin secretion in plasma cells: directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression.[UniProtKB/Swiss-Prot Function]