DNER (NM_139072) Human Tagged ORF Clone

CAT#: RC206164

DNER (Myc-DDK-tagged)-Human delta/notch-like EGF repeat containing (DNER)

Product Images

Circular map for RC206164

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNER
• Synonyms: bet; UNQ26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206164 representing NM_139072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCCGCCGCGCCCAGGCGCCCGGTGCGCAGCTGCTGCCCGCGCTGGCCCTGCTGCTGCTGCTGC
  TCGGAGCGGGGCCCCGAGGCAGCTCCCTGGCCAACCCGGTGCCCGCCGCGCCCCTGTCTGCGCCCGGGCC
  GTGCGCCGCGCAGCCCTGCCGGAATGGGGGTGTGTGCACCTCGCGCCCTGAGCCGGACCCGCAGCACCCG
  GCCCCCGCCGGCGAGCCTGGCTACAGCTGCACCTGCCCCGCCGGGATCTCCGGCGCCAACTGCCAGCTTG
  TTGCAGATCCTTGTGCCAGCAACCCTTGTCACCATGGCAACTGCAGCAGCAGCAGCAGCAGCAGCAGCGA
  TGGCTACCTCTGCATTTGCAATGAAGGCTATGAAGGTCCCAACTGTGAACAGGCACTTCCCAGTCTCCCA
  GCCACTGGCTGGACCGAATCCATGGCACCCCGACAGCTTCAGCCTGTTCCTGCTACTCAGGAGCCTGACA
  AAATCCTGCCTCGCTCTCAGGCAACGGTGACACTGCCTACCTGGCAGCCGAAAACAGGGCAGAAAGTTGT
  AGAAATGAAATGGGATCAAGTGGAGGTGATCCCAGATATTGCCTGTGGGAATGCCAGTTCTAACAGCTCT
  GCGGGTGGCCGCCTGGTATCCTTTGAAGTGCCACAGAACACCTCAGTCAAGATTCGGCAAGATGCCACTG
  CCTCACTGATTTTGCTCTGGAAGGTCACGGCCACAGGATTCCAACAGTGCTCCCTCATAGATGGACGAAG
  TGTGACCCCCCTTCAGGCTTCAGGGGGACTGGTCCTCCTGGAGGAGATGCTCGCCTTGGGGAATAATCAC
  TTTATTGGTTTTGTGAATGATTCTGTGACTAAGTCTATTGTGGCTTTGCGCTTAACTCTGGTGGTGAAGG
  TCAGCACCTGTGTGCCGGGGGAGAGTCACGCAAATGACTTGGAGTGTTCAGGAAAAGGAAAATGCACCAC
  GAAGCCGTCAGAGGCAACTTTTTCCTGTACCTGTGAGGAGCAGTACGTGGGTACTTTCTGTGAAGAATAC
  GATGCTTGCCAGAGGAAACCTTGCCAAAACAACGCGAGCTGTATTGATGCAAATGAAAAGCAAGATGGGA
  GCAATTTCACCTGTGTTTGCCTTCCTGGTTATACTGGAGAGCTTTGCCAGTCCAAGATTGATTACTGCAT
  CCTAGACCCATGCAGAAATGGAGCAACATGCATTTCCAGTCTCAGTGGATTCACCTGCCAGTGTCCAGAA
  GGATACTTCGGATCTGCTTGTGAAGAAAAGGTGGACCCCTGCGCCTCGTCTCCGTGCCAGAACAACGGCA
  CCTGCTATGTGGACGGGGTACACTTTACCTGCAACTGCAGCCCGGGCTTCACAGGGCCGACCTGTGCCCA
  GCTTATTGACTTCTGTGCCCTCAGCCCCTGTGCTCATGGCACGTGCCGCAGCGTGGGCACCAGCTACAAA
  TGCCTCTGTGATCCAGGTTACCATGGCCTCTACTGTGAGGAGGAATATAATGAGTGCCTCTCCGCTCCAT
  GCCTGAATGCAGCCACCTGCAGGGACCTCGTTAATGGCTATGAGTGTGTGTGCCTGGCAGAATACAAAGG
  AACACACTGTGAATTGTACAAGGATCCCTGCGCTAACGTCAGCTGTCTGAACGGAGCCACCTGTGACAGC
  GACGGCCTGAATGGCACGTGCATCTGTGCACCCGGGTTTACAGGTGAAGAGTGCGACATTGACATAAATG
  AATGTGACAGTAACCCCTGCCACCATGGTGGGAGCTGCCTGGACCAGCCCAATGGTTATAACTGCCACTG
  CCCGCATGGTTGGGTGGGAGCAAACTGTGAGATCCACCTCCAATGGAAGTCCGGGCACATGGCGGAGAGC
  CTCACCAACATGCCACGGCACTCCCTCTACATCATCATTGGAGCCCTCTGCGTGGCCTTCATCCTTATGC
  TGATCATCCTGATCGTGGGGATTTGCCGCATCAGCCGCATTGAATACCAGGGTTCTTCCAGGCCAGCCTA
  TGAGGAGTTCTACAACTGCCGCAGCATCGACAGCGAGTTCAGCAATGCCATTGCATCCATCCGGCATGCC
  AGGTTTGGAAAGAAATCCCGGCCTGCAATGTATGATGTGAGCCCCATCGCCTATGAAGATTACAGTCCTG
  ATGACAAACCCTTGGTCACACTGATTAAAACTAAAGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206164 representing NM_139072
  Red=Cloning site Green=Tags(s)
  
  MQPRRAQAPGAQLLPALALLLLLLGAGPRGSSLANPVPAAPLSAPGPCAAQPCRNGGVCTSRPEPDPQHP
  APAGEPGYSCTCPAGISGANCQLVADPCASNPCHHGNCSSSSSSSSDGYLCICNEGYEGPNCEQALPSLP
  ATGWTESMAPRQLQPVPATQEPDKILPRSQATVTLPTWQPKTGQKVVEMKWDQVEVIPDIACGNASSNSS
  AGGRLVSFEVPQNTSVKIRQDATASLILLWKVTATGFQQCSLIDGRSVTPLQASGGLVLLEEMLALGNNH
  FIGFVNDSVTKSIVALRLTLVVKVSTCVPGESHANDLECSGKGKCTTKPSEATFSCTCEEQYVGTFCEEY
  DACQRKPCQNNASCIDANEKQDGSNFTCVCLPGYTGELCQSKIDYCILDPCRNGATCISSLSGFTCQCPE
  GYFGSACEEKVDPCASSPCQNNGTCYVDGVHFTCNCSPGFTGPTCAQLIDFCALSPCAHGTCRSVGTSYK
  CLCDPGYHGLYCEEEYNECLSAPCLNAATCRDLVNGYECVCLAEYKGTHCELYKDPCANVSCLNGATCDS
  DGLNGTCICAPGFTGEECDIDINECDSNPCHHGGSCLDQPNGYNCHCPHGWVGANCEIHLQWKSGHMAES
  LTNMPRHSLYIIIGALCVAFILMLIILIVGICRISRIEYQGSSRPAYEEFYNCRSIDSEFSNAIASIRHA
  RFGKKSRPAMYDVSPIAYEDYSPDDKPLVTLIKTKDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139072
• ORF Size: 2211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139072.4
• RefSeq Size: 3272 bp
• RefSeq ORF: 2214 bp
• Locus ID: 92737
• UniProt ID: Q8NFT8
• Cytogenetics: 2q36.3
• Domains: EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Transmembrane
• MW: 78.9 kDa
• Gene Summary: Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis (By similarity).[UniProtKB/Swiss-Prot Function]